Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Zhivkova-Galunska, Maria; Adwan, Hassan; Eyol, Ergül; Kleeff, Jörg; Kolb, Armin; Bergmann, Frank; Berger, Martin R.

doi:10.4161/cbt.10.1.12161

Cited by 35 publications

(34 citation statements)

References 63 publications

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“…Since previous studies showed that treatment of pancreatic cancer cell lines with exogenous osteopontin did not affect proliferation, these data suggest that endogenous levels of osteopontin in this cell line are necessary and sufficient for the support of tumor cell growth. 7,8 Since osteopontin can also increase the invasiveness of pancreatic carcinoma cells, these data reinforce the concept that osteopontin may be a target for therapeutic intervention. 8 The authors also demonstrate that knock down of osteonectin mRNA, using an antisense oligonucleotide, resulted in increased cell growth, whereas growth was suppressed by exogenously added osteonectin.…”

supporting

confidence: 66%

“…6 The matricellular proteins osteopontin [ In this issue of Cancer Biology & Therapy, Zhivkova-Galunska and coworkers examine the relative expression of osteopontin and osteonectin transcripts in a panel of 14 human pancreatic cancer cell lines, and determine whether there is any correlation between these RNA levels and characteristics associated with aggressive cancer phenotype. 7 Briefly, the authors find a correlation between higher levels of osteopontin mRNA and the ability of the cell lines to form tumors in the liver of nude rats. In a human pancreatic adenocarcinoma cell line, knock down of osteopontin mRNA, via transient transfection of a specific antisense oligonucleotide, suppressed cell growth.…”

mentioning

confidence: 99%

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Matricellular proteins osteopontin and osteonectin/SPARC in pancreatic carcinoma

Delany

2010

Cancer Biology & Therapy

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supporting

confidence: 66%

mentioning

confidence: 99%

Matricellular proteins osteopontin and osteonectin/SPARC in pancreatic carcinoma

Delany

2010

Cancer Biology & Therapy

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“…Osteopontin (OPN) is a matricellular protein that participates in a wide range of physiological and pathologic processes, including tumorigenesis and metastasis [13,14,15,16]. Recent studies showed that OPN is involved in the tumorigenesis of breast cancer [17,18,19], hepatic carcinoma [20], gastric cancer [21].…”

Section: Introductionmentioning

confidence: 99%

OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort

Wang

Cai

et al. 2013

Cell Physiol Biochem

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Aim: to test the possible association between the polymorphism of Osteopontin (OPN) gene with the risk and the clinical features of papillary thyroid cancer (PTC). Methods: A total of 363 PTC patients and 413 healthy controls were enrolled. OPN expressions in tumor tissue were detected by immunohistochemistry. OPN gene polymorphisms, namely, -66T>G (rs28357094), -156G>GG (rs17524488), and -443C>T (rs11730582), were determined. Results: We observed that the PTC patients had significantly higher rates of -443TT genotypes than controls (P<0.001). The multivariate logistic regression analysis showed a significantly increased risk for PTC for the -443CC genotype compared with the -443TT genotype (adjusted OR= 4.312, 95%CI: 2.747 -6.987, adjusted P < 0.001). OPN protein was not expressed in normal thyroid tissues while tumor samples from PTC patients were shown to have high expressions of OPN. Also, we found that the high OPN expressions were significantly more prevalent in -443CC carriers than TT carriers (P <0.001). Both the CC carriers and OPN expression were closely associated with the cervical lymph node metastasis and angiolymphatic invasion of PTC. Conclusion: This study provides evidence to support the connection between the OPN genetic polymorphism and tissue expression with risk as well as the invasiveness of PTC.

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“…Increased gene expression in response to LF has been observed by other authors as well. 30 An even more intensive induction of Opn expression was caused upon implantation of CC531 lacZ cells into the rat liver (Figure 3c, lane 3). We therefore hypothesized that silencing of Opn would result in an anti-metastatic effect.…”

Section: Discussionmentioning

confidence: 95%

Influence of osteopontin expression on the metastatic growth of CC531 rat colorectal carcinoma cells in rat liver

et al. 2011

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Development of hepatic metastasis is responsible for most of colorectal cancer-related deaths. Osteopontin (OPN) is a small integrin-binding N-linked glycoprotein, which plays a crucial role in the formation of hepatic metastasis. This study aimed to suppress Opn expression by an antisense-oligonucleotide (ASO Opn ) to decrease liver metastasis in vivo. The effect of ASO Opn was investigated in vitro in CC531 lacZ colorectal cancer cells in comparison to sense (SO) or nonsense (NSO) oligomers, by determining mRNA and protein expression levels, as well as cell survival. For in vivo treatment, CC531 lacZ cells were intraportally inoculated into rats to compare the effects of ASO, SO and NSO oligomers, following prolonged subcutaneous administration by osmotic minipumps. The resulting CC531 lacZ tumor cell load in the liver was measured by a b-galactosidase assay. Proliferation of CC531 lacZ cells in vitro was significantly decreased after ASO Opn and SO treatment (Po0.001). Liver metastasis development was reduced as long as ASO Opn was administered, but this effect was rapidly blunted following the end of the ASO Opn administration. In contrast, administration of the SO resulted in a tumor load reduction, which surprisingly surpassed the ASO Opn effect in vivo in terms of a long-lasting metastasis suppression, which was accompanied with increased survival of the animals. Administration of the ASO Opn in rats was effective in decreasing their liver metastasis. The short-lived effect might be extended by modifications suited to increase the ASOs' half-life. In addition, there was a superior anti-metastatic effect caused by the SO, which has not been reported previously.

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Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Cited by 35 publications

References 63 publications

Matricellular proteins osteopontin and osteonectin/SPARC in pancreatic carcinoma

Matricellular proteins osteopontin and osteonectin/SPARC in pancreatic carcinoma

OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort

Influence of osteopontin expression on the metastatic growth of CC531 rat colorectal carcinoma cells in rat liver

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