Osteopontin and integrin are involved in cholesterol gallstone formation

Yang, Lin; Chen, Jinhong; Cai, Duan; Wang, Liying; Zha, Xiliang

doi:10.12659/msm.882194

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…Our previous research revealed that OPN can inhibit cholesterol gallstone formation as an anti-nucleation factor in gallbladder bile, and OPN in gallbladder bile is probably from gallbladder tissues 16 17 . In the current study, we found that the expression of hepatic OPN was increased in GS, and OPN deficiency altered biliary homeostasis and protected against gallstone formation in mice.…”

Section: Discussionmentioning

confidence: 98%

“…Osteopontin (OPN) is a soluble cytokine and a matrix-associated protein expressed in the majority of tissues and body fluids 14 and is able to control tumour progression and metastasis 15 . Our previous studies demonstrated that OPN can inhibit cholesterol gallstone formation as an anti-nucleation factor in gallbladder bile 16 17 . Another study showed that OPN was highly expressed in the epithelium of stone-laden intrahepatic bile ducts, intramural, extramural glands and stones, indicating that OPN is involved in hepatolithiasis 18 .…”

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confidence: 99%

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Osteopontin Deficiency Alters Biliary Homeostasis and Protects against Gallstone Formation

Lin

Lü

Shao

et al. 2016

Sci Rep

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The precipitation of excess biliary cholesterol as solid crystals is a prerequisite for cholesterol gallstone formation, which occurs due to disturbed biliary homeostasis. Biliary homeostasis is regulated by an elaborate network of genes in hepatocytes. If unmanaged, the cholesterol crystals will aggregate, fuse and form gallstones. We have previously observed that the levels of osteopontin (OPN) in bile and gallbladder were reduced in gallstone patients. However, the role and mechanism for hepatic OPN in cholesterol gallstone formation is undetermined. In this study, we found that the expression of hepatic OPN was increased in gallstone patients compared with gallstone-free counterparts. Then, we observed that OPN-deficient mice were less vulnerable to cholesterol gallstone formation than wild type mice. Further mechanistic studies revealed that this protective effect was associated with alterations of bile composition and was caused by the increased hepatic CYP7A1 expression and the reduced expression of hepatic SHP, ATP8B1, SR-B1 and SREBP-2. Finally, the correlations between the expression of hepatic OPN and the expression of these hepatic genes were validated in gallstone patients. Taken together, our findings reveal that hepatic OPN contributes to cholesterol gallstone formation by regulating biliary metabolism and might be developed as a therapeutic target for gallstone treatments.

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Osteopontin Deficiency Alters Biliary Homeostasis and Protects against Gallstone Formation

Lin

Lü

Shao

et al. 2016

Sci Rep

Self Cite

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“…The cholesterol gallstone pathogenesis is considered multifactorial, and though OPN has been found in normal humans’ gallbladder bile and tissue, the role of OPN in forming GSs is still unknown [ 43 ]. In the current study, there is a relatively higher detection of mRNA OPN expression in hepatic tissue in the GIII when compared with the GII, GIV, and GI.…”

Section: Discussionmentioning

confidence: 99%

Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Abdal,

Naemi

2023

J Adv Vet Anim Res

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Objective: This research was designed to assess the influence of the administration of a lithogenic diet, hydrogen peroxide, and vitamin AD3E on rabbits’ gallstone formation and to envisage the expression of osteopontin (OPN) in their hepatic tissues. Materials and Methods: Twenty-four healthy local mature rabbits of both genders were divided into four equal groups. At the end of the feeding period, samples of blood were taken from all rab¬bits after they had fasted overnight to estimate the serum lipid profile. And some of the hepatic tissue has been preserved at −28°C for molecular analysis and gene expression. Results: The gallstones were formed 100% in the GIII and 50% in the GIV. The mRNA OPN expression showed a significant increase in the GIII when compared with other groups. In Groups III and II, the serum levels of total cholesterol, Triglyceride, L-C, low-density lipoprotein-choles, and VLDL-C were significantly increased when compared with GI, while in GIII, the serum level of high-density lipoprotein-cholesterol was significantly decreased when compared with GI. Conclusion: It was concluded that the expression of the mRNA OPN was increased in the hepatic tissue of gallstone-formed rabbits.

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Effect of magnesium ammonium phosphate on the expression of adhesion molecules in sheep renal tubular epithelial cells

Ruonan

Zou

Tian

et al. 2021

Research in Veterinary Science

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Osteopontin and integrin are involved in cholesterol gallstone formation

Cited by 7 publications

References 41 publications

Osteopontin Deficiency Alters Biliary Homeostasis and Protects against Gallstone Formation

Osteopontin Deficiency Alters Biliary Homeostasis and Protects against Gallstone Formation

Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Effect of magnesium ammonium phosphate on the expression of adhesion molecules in sheep renal tubular epithelial cells

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