Osteomimetic matrix components alter cell migration and drug response in a 3D tumour-engineered osteosarcoma model

Pavlou, Marina; Shah, Mittal; Gikas, Panagiotis D.; Briggs, Timothy; Roberts, Scott J.; Cheema, Umber

doi:10.1016/j.actbio.2019.07.011

Cited by 27 publications

(47 citation statements)

References 56 publications

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“…Laminins have demonstrated to enhance cell adhesion in OS cell lines [ 40 ], with high laminin-adherent OS cells showing notably more invasiveness than their low laminin-adherent counterparts [ 41 ]. In a work which implemented a 3D OS cell line model, a matrix supplemented with laminin led to an increased invasion of OS cells into the surrounding acellular bone marrow environment [ 42 ].…”

Section: Ecm Components and Their Function In Osmentioning

confidence: 99%

The role of extracelluar matrix in osteosarcoma progression and metastasis

Cui

Dean

Hornicek

et al. 2020

J Exp Clin Cancer Res

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Osteosarcoma (OS) is the most common primary bone malignancy and responsible for considerable morbidity and mortality due to its high rates of pulmonary metastasis. Although neoadjuvant chemotherapy has improved 5-year survival rates for patients with localized OS from 20% to over 65%, outcomes for those with metastasis remain dismal. In addition, therapeutic regimens have not significantly improved patient outcomes over the past four decades, and metastases remains a primary cause of death and obstacle in curative therapy. These limitations in care have given rise to numerous works focused on mechanisms and novel targets of OS pathogenesis, including tumor niche factors. OS is notable for its hallmark production of rich extracellular matrix (ECM) of osteoid that goes beyond simple physiological growth support. The aberrant signaling and structural components of the ECM are rich promoters of OS development, and very recent works have shown the specific pathogenic phenotypes induced by these macromolecules. Here we summarize the current developments outlining how the ECM contributes to OS progression and metastasis with supporting mechanisms. We also illustrate the potential of tumorigenic ECM elements as prognostic biomarkers and therapeutic targets in the evolving clinical management of OS.

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Section: Ecm Components and Their Function In Osmentioning

confidence: 99%

The role of extracelluar matrix in osteosarcoma progression and metastasis

Cui

Dean

Hornicek

et al. 2020

J Exp Clin Cancer Res

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“…Fibronectin and laminin are glycoproteins that integrate with adhesion molecules or cell membrane receptors to mediate cell adhesion, proliferation, differentiation, apoptosis, and migration by inducing a number of signalling pathways [22] , [23] . Upregulated fibronectin-1 and laminin contribute to the doxorubicin resistance of OS cells, and high fibronectin-1 expression is correlated with poor overall survival after surgical resection in OS patients [24] , [25] . High-grade OS tissues express high levels of fibronectin and its receptor syndecan 4, which are positively correlated with lung metastasis and short overall survival [26] .…”

Section: The Tme Landscape Of Osmentioning

confidence: 99%

Role of microRNAs in the crosstalk between osteosarcoma cells and the tumour microenvironment

Lei

Chen

Yongcan

et al. 2020

Journal of Bone Oncology

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Section: In Vitro 3d Modelsmentioning

confidence: 99%

“…ECM is a major component of the tumour microenvironment, with studies showing that it acts in a pro-tumorigenic capacity, enhancing OS cell invasion, with some evidence suggesting it provides a protective niche for drug resistance [ 56 ]. This has been studied mainly in 2D in vitro assays using multiple components of ECM, including fibroblasts [ 57 ] and acellular proteins [ 56 ]. There have also been few 3D models incorporating components of the ECM, including the use of collagen sponges [ 49 ] and growth factors [ 58 ] to simulate aspects of the tumour microenvironment.…”

Section: In Vitro 3d Modelsmentioning

confidence: 99%

The Role of Pre-Clinical 3-Dimensional Models of Osteosarcoma

Smith

Beers

Gray

et al. 2020

IJMS

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Treatment for osteosarcoma (OS) has been largely unchanged for several decades, with typical therapies being a mixture of chemotherapy and surgery. Although therapeutic targets and products against cancer are being continually developed, only a limited number have proved therapeutically active in OS. Thus, the understanding of the OS microenvironment and its interactions are becoming more important in developing new therapies. Three-dimensional (3D) models are important tools in increasing our understanding of complex mechanisms and interactions, such as in OS. In this review, in vivo animal models, in vitro 3D models and in ovo chorioallantoic membrane (CAM) models, are evaluated and discussed as to their contribution in understanding the progressive nature of OS, and cancer research. We aim to provide insight and prospective future directions into the potential translation of 3D models in OS.

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Osteomimetic matrix components alter cell migration and drug response in a 3D tumour-engineered osteosarcoma model

Cited by 27 publications

References 56 publications

The role of extracelluar matrix in osteosarcoma progression and metastasis

The role of extracelluar matrix in osteosarcoma progression and metastasis

Role of microRNAs in the crosstalk between osteosarcoma cells and the tumour microenvironment

The Role of Pre-Clinical 3-Dimensional Models of Osteosarcoma

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