Osteoinductivity of polycaprolactone nanofibers grafted functionalized with carboxymethyl chitosan: Synergic effect of β-carotene and electromagnetic field

Shapourzadeh, Atena; Atyabi, Seyed-Mohammad; Irani, Shiva; Bakhshi, Hadi

doi:10.1016/j.ijbiomac.2020.02.036

Cited by 26 publications

(33 citation statements)

References 62 publications

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“…Osteoconductive organic or inorganic agents addition into the PCL substrates is an efficient manner to increase the bioactivity of scaffolds 4 . Carboxymethyl chitosan (CMC) with high aqueous solubility, controllable biodegradability, antibacterial activity, and osteogenesis‐inducing potency, is a promising biopolymer for bone healing 5,6 . Recently, synthetic silicates have exposed a high degree of functionality for promoting osteodifferentiation and bone healing 7 .…”

Section: Introductionmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

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Section: Introductionmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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“…Carboxymethyl chitosan (CMC) is a water‐soluble derivative of chitosan with hydrophilicity, antibacterial, and biomineralization properties. Despite the interesting biological and chemical properties, CMC lacks sufficient osteoinductive activity 7,8 …”

Section: Introductionmentioning

confidence: 99%

Immobilization of cobalt‐loaded laponite/carboxymethyl chitosan on polycaprolactone nanofiber for improving osteogenesis and angiogenesis activities

Arab‐Ahmadi

Irani

Bakhshi

et al. 2021

Polymers for Advanced Techs

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Designing scaffolds with therapeutic potential to stimulate angiogenesis and osteogenesis is considered a promising strategy in the regeneration of large skeletal defects. In this study, the cobalt ions (Co2+) were incorporated in laponite (LAP) nanodiscs (0.65, 1.3, and 2.6 g/L) and, in combination with carboxymethyl chitosan (CMC), were immobilized on the electrospun polycaprolactone (PCL) nanofiber to fabricate scaffolds for simultaneous enhancing osteogenesis and angiogenesis inductions to human bone marrow mesenchymal stem cells. The Fourier transform infrared, energy dispersive X‐ray, and inductively coupled plasma atomic emission spectrometries confirmed the Co2+‐loading on the LAP nanodiscs and the chemical immobilization on the surface of PCL nanofibers. The result of MTT assay, scanning electron microscopy, and fluorescence staining displayed that Co2+‐loaded scaffolds were biocompatible. Furthermore, the incorporation of Co2+ ions into LAP nanodiscs had significant effects on the calcium deposition and expression of osteogenic markers, alkaline phosphatase and osteonectin, as well as, the expression of angiogenic markers, hypoxia‐inducible factor‐1α and vascular endothelial growth factor of the cells seeded on the scaffolds (p ≤ 0.05). These results suggested that the combination of Co2+ ions with CMC/LAP could result in a synergetic effect on the osteogenesis and angiogenesis stimulations.

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“…Synthetic [i.e., poly(ε‐caprolactone)] and natural polymers (i.e., chitosan, collagen, alginate, agarose, hyaluronic acid, and fibrin) have been largely proposed to manufacture scaffolds for hard and soft tissue regeneration. [ 4–7 ]…”

Section: Introductionmentioning

confidence: 99%

“…A scaffold may be considered as an interconnected porous structure, which is able to support cell adhesion, proliferation, and differentiation, also promoting the extracellular matrix analogue deposition necessary for tissue regeneration. [ 4–7 ]…”

Section: Introductionmentioning

confidence: 99%

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

Santis

Russo

Fucile

et al. 2021

Macromolecular Symposia

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Light‐activated resins and composites are used in conjunction with a light curing unit and allow an on‐demand process of polymerization. These kinds of materials usually represent the most popular choice in the restorative dental practice. Some works have already highlighted contemporary tendencies in the use of nondegradable scaffolds and mesenchymal stem cells in regenerative medicine. Accordingly, the aim of the current research is to develop 3D porous and light‐activated composite structures with optimized functional properties. Preliminary mechanical and biological tests are carried out.

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Osteoinductivity of polycaprolactone nanofibers grafted functionalized with carboxymethyl chitosan: Synergic effect of β-carotene and electromagnetic field

Cited by 26 publications

References 62 publications

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Immobilization of cobalt‐loaded laponite/carboxymethyl chitosan on polycaprolactone nanofiber for improving osteogenesis and angiogenesis activities

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

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