Osteoinductivity depends on the ratio of demineralized bone matrix to acellular dermal matrix in defects in rat skulls

Kim, Jin; Kwang-Won, Lee; Ahn, Jae-Hyoung; Kim, Jin‐Young; Lee, Tae-Yeon; Choi, Bohm

doi:10.1007/s13770-012-1083-4

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…ADM is biocompatible, and biodegradable, and does not inhibit the bone formation capacity of DBM, which allows for tissue regeneration. 26,27 The ADM scaffold possesses strong thermal stability with a T onset of 47 C (much higher than human body temperature), which indicates that the ADM would not be biodegraded rapidly upon implantation. Histological analysis of ADM has demonstrated that it preserves intact collagen bundles and extracellular structure (Figure 5B).…”

Section: Discussionmentioning

confidence: 99%

“…ADM microfibers are derived from the human dermis by decellularization while preserving the structural integrity and biochemical composition. ADM is biocompatible, and biodegradable, and does not inhibit the bone formation capacity of DBM, which allows for tissue regeneration 26,27 . The ADM scaffold possesses strong thermal stability with a T onset of 47°C (much higher than human body temperature), which indicates that the ADM would not be biodegraded rapidly upon implantation.…”

Section: Discussionmentioning

confidence: 99%

“…The most commonly used collagenous carrier is the purified collagen 24,25 . Particulate human acellular dermal matrix (ADM) improved the handling and showed its superiority as DBM carriers 26,27 . Nevertheless, the mixing process of granular ADM and DBM particles before implantation increases surgical time and introduces the risks of non‐uniform mixing and contamination.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Ma,

Ren,

Wang

et al. 2023

J Biomed Mater Res

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Allogenic demineralized bone matrix (DBM), processed to expose bioactive proteins imbedded by calcium salts, is widely used for bone repair and regeneration as an alternative to the autologous bone graft. However, demineralized bone matrices from tissue banks vary significantly in residual calcium content and osteogenicity for clinical bone regeneration. The present study produced DBM with various residual calcium contents by partial demineralization using ethylenediaminetetraacetic acid disodium (EDTA) and hydrochloric acid. Compositional analysis reveals that, as the percent weight loss of bone materials increases from 0% to 74.9% during demineralization, the residual calcium content of DBM decreases from 24.8% to 0.2% and collagen content increases from 29.7% to 92.6%. Calorimetrical analysis and Fourier transform infrared (FTIR) analysis demonstrated that demineralization to the residual calcium content of <4% enables the complete exposure and/or release of bone collagen fibers and other bioactive molecules. In order to evaluate the relationship between the extent of demineralization and the osteogenicity of DBM, DBM particles were fabricated with the aid of acellular dermal matrix (ADM) microfibers to form flexible foam‐like DBM/ADM composites. Proteomic analysis identified various type collagens and bone formation‐related bioactive molecules in both ADM and DBM. Using the rat bilateral Φ = 5 mm calvarium defect repair model, the study had shown that the DBM/ADM composite with ~20% DBM residual calcium (e.g., ~40% calcium being removed) maximized the osteogenicity for bone defect repair after 4 and 8 weeks. DBM with ~40% calcium removal had the maximal osteogenicity presumably through the sustained release of bioactive molecules during the process of bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Ma,

Ren,

Wang

et al. 2023

J Biomed Mater Res

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“…On the other hand, successful healing of bone defects reported after BMG implantation has encouraged researchers to use this material as a synthetic alternative to autogenous bone grafts [19,20]. Bone matrix gelatin contains type I collagen as well as a high protein content including Bone Morphogenetic Proteins (BMPs), which induce the differentiation of mesenchymal cells to osteoblasts at the site of bone defects [21].…”

Section: Introductionmentioning

confidence: 99%

Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

Aval¹,

Saberi²,

Sargolzaei³

et al. 2018

Preprint

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Objective: Regeneration of bone defects remains a challenge for maxillofacial and reparative surgeons. The purpose of this histological study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination in artificially created mandibular bone defects in rats. The quality of the newly formed bone was also evaluated.Methods: Thirty-six male Sprague Dawley rats (6-8 weeks old with 120-150 g weight) were randomly divided into four groups. Defects (3 mm in diameter and 2 mm in depth) were created in the mandible of rats and filled with 6 mg of OCP, BMG or a combination of both (1/4 ratio), respectively. Defects were left unfilled in the control group. To assess osteoinduction and bone regeneration and determine the quality of the newly formed bone, tissue specimens were harvested at seven, 14, and 21 days post-implantation. The specimens were processed, stained with hematoxylin and eosin (H&E) and histologically analyzed under light microscopy. Results:In the experimental groups, new bone formation was initiated at the margins of defects from seventh day after implantation. At the end of the study period, the amount of the newly formed bone increased and the bone was relatively mature. Osteoinduction and new bone formation were greater in OCP/BMG group. In the control group, slight amount of new bone had been formed at the defect margins (next to host bone) on day 21. Conclusion:Combination of OCP/BMG may serve as an optimal biomaterial for treatment of mandibular bone defects.

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5. Demineralized Bone Matrix (DBM) and Bone Grafts

Kim¹,

Oh²

2016

Translating Biomaterials for Bone Graft

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Osteoinductivity depends on the ratio of demineralized bone matrix to acellular dermal matrix in defects in rat skulls

Cited by 3 publications

References 14 publications

Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

5. Demineralized Bone Matrix (DBM) and Bone Grafts

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