Osteoinductive Activity of Composites of Bone Morphogenetic Protein and Pure Titanium

Kawai, Takeshi; Mieki, Akimichi; Ohno, Y.; Umemura, Masataka; Kataoka, Hiroto; Kurita, Shinya; Koie, Masato; Jinde, Toshikage; Hasegawa, Jiro; Urist, Marshall R.

doi:10.1097/00003086-199305000-00038

Cited by 52 publications

(27 citation statements)

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“…However, conventional micro-CT requires long scanning times, which leads to significant effects of radiation in the study animals. Furthermore, the impact that the prolonged and repetitive use of anesthesia has on bone formation cannot be ignored1 [5][6][7][8][9][10][11][12][13][14][15][16][17] . On the other hand,…”

Section: Continuous Micro-ct Scanning Of a Living Mousementioning

confidence: 99%

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Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Sato

Hayashi

Hamazima

et al. 2013

J. Hard Tissue Biology.

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Numerous disorders and types of injuries require bone tissue regeneration for the repair of bone defects. Previous attempts have been made to apply bone morphogenetic protein (BMP), a bioactive growth factor, to the defect site for bone reconstruction in various animal models. However, many of these studies evaluated the efficacy of BMP using texture and soft X-ray imaging performed after the laboratory animal was euthanized. As such, any attempts to observe the induction of new bone formation over a continuous time scale within the same laboratory animal has been difficult. Accordingly, a setup in which BMP-induced ectopic bone formation is assessed over the long term has become extremely important. To date, there are no reports to verify the long-term preservation of BMP-induced bone formation at sites of repair. In this study, we performed a long-term and continuous observation of new bone formation after the addition of BMP using a new threedimensional micro X-ray computed tomography technology, R_mCT. BMP was implanted into a gap in the fascia of the femur in a mouse, and scanning using this new technology was continued for 320 days. The following tests were performed: (1) observation using 3-dimensional construction imaging; (2) measurement of the mean ratio of new bone volume to total volume, and (3) measurement of the density of new bone formation. From these tests, we observed that BMP-induced ectopic bone persists for up to 320 days, with minimal losses in bone volume or density, unlike previously accepted notions. We also present details of the structural changes in this ectopic bone.

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Section: Continuous Micro-ct Scanning Of a Living Mousementioning

confidence: 99%

“…Bone morphogenetic protein1-3), which is a bioactive factor, has been traditionally used to induce bone reconstruction in conjunction with various biomaterials [4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Sato

Hayashi

Hamazima

et al. 2013

J. Hard Tissue Biology.

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“…Recently, the function of individual BMPs on various cell types, cultured with various biomaterials has been extensively tested44, 102,113,125,170,171,176,177). In vivo experiments surprisingly showed that BMP-2 (probably model-related) can inhibit176) or increase125) bone ingrowth.…”

Section: Genetic Potential Of Osteopromotive Growth Factors On Implanmentioning

confidence: 99%

Genetic Potential of Interfacial Guided Osteogenesis in Implant Devices.

Letic-Gavrilovic

Scandurra

Abe

2000

Dental Materials Journal

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“…17) Various materials as a BMP carrier have already been examined, such as -TCP, 18) hydroxyapatite, 19) biphasic calcium phosphate, 20) collagen, 21) polylactic acid-polyglycolic acid copolymer, 22) and titanium. 23) Several studies 18,[24][25][26] indicate that -TCP can be used as a slow-release delivery system for BMP and potentiates the activity of BMP. In this study, we examined cages filled with -TCP and rhBMP-2 to improve the success rate for bony fusion.…”

Section: Introductionmentioning

confidence: 99%

β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

et al. 2004

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The use of -tricalcium phosphate (-TCP) as osteoconductive and bone morphogenetic protein-2 (BMP-2) as osteoinductive bone graft substitutes has recently gained considerable research interest in spine surgery. However, whether the combination can be extrapolated to a successful interbody spinal arthrodesis remains uncertain. In this study, -TCP combined with recombinant human BMP-2 was examined in the canine lumbar spine model as a substitute for autograft used for packing interbody fusion cages. The discectomy and interbody cage fusion were performed at three disc spaces in 8 dogs. The examination of microradiographs and histological sections of the lumbar spine at 16 weeks postsurgery revealed three fusions in autograft group (A), three -TCP group (B), and five in -TCP-BMP-2 (C). The mean percentage of trabecular bone area in the cages was 51.9% in group A, 48.8% in group B and 65.6% in group C. Statistical analyses of the results could not give significance. The young animal model used in this study might suppress difference between the groups. As mentioned, however, actual data of mean percentage of trabecular bone formation and of mechanical stiffness were largest in the cages filled with -TCP and BMP-2. In clinical settings, we expect that a cage filled with -TCP and BMP gives beneficial effects especially for patients with poor fusion statuses.

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Osteoinductive Activity of Composites of Bone Morphogenetic Protein and Pure Titanium

Cited by 52 publications

References 0 publications

Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Genetic Potential of Interfacial Guided Osteogenesis in Implant Devices.

β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

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Osteoinductive Activity of Composites of Bone Morphogenetic Protein and Pure Titanium

Cited by 52 publications

References 0 publications

Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Long-Term Observation of Ectopic Bone Formation Using In Vivo Microcomputed Tomography

Genetic Potential of Interfacial Guided Osteogenesis in Implant Devices.

&beta;-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

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β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine