Osteoinduction Using Bone Morphogenic Protein in Irradiated Tissue

Howard, Brian K.; Brown, Karla R.; Leach, Joseph L.; Chang, Cheng Hui; Rosenthal, David I.

doi:10.1001/archotol.124.9.985

Cited by 40 publications

(29 citation statements)

References 24 publications

(25 reference statements)

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“…Irradiation of up to 8 Gy did not affect the BMP-2-induced osteoblast differentiation of C2C12 cells in vitro (Ikeda et al, 2000). New bone was formed on BMP-2-treated hydroxyapatite disks implanted into a subperiosteal pocket in rabbits radiated pre-operatively with a fractionated dose of 20 Gy (Howard et al, 1998). Despite these encouraging findings, 3-mm rat calvarial defects preoperatively treated with a 12-Gy radiation dose, and subsequently with BMP-2, had successful bone regeneration, but incomplete healing of the defect (Wurzler et al, 1998).…”

Section: (53) Craniofacial Bone Regeneration After Ablative Cancer Smentioning

confidence: 99%

Craniofacial Tissue Engineering by Stem Cells

et al. 2006

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Craniofacial tissue engineering promises the regeneration or de novo formation of dental, oral, and craniofacial structures lost to congenital anomalies, trauma, and diseases. Virtually all craniofacial structures are derivatives of mesenchymal cells. Mesenchymal stem cells are the offspring of mesenchymal cells following asymmetrical division, and reside in various craniofacial structures in the adult. Cells with characteristics of adult stem cells have been isolated from the dental pulp, the deciduous tooth, and the periodontium. Several craniofacial structures-such as the mandibular condyle, calvarial bone, cranial suture, and subcutaneous adipose tissue-have been engineered from mesenchymal stem cells, growth factor, and/or gene therapy approaches. As a departure from the reliance of current clinical practice on durable materials such as amalgam, composites, and metallic alloys, biological therapies utilize mesenchymal stem cells, delivered or internally recruited, to generate craniofacial structures in temporary scaffolding biomaterials. Craniofacial tissue engineering is likely to be realized in the foreseeable future, and represents an opportunity that dentistry cannot afford to miss.

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Section: (53) Craniofacial Bone Regeneration After Ablative Cancer Smentioning

confidence: 99%

Craniofacial Tissue Engineering by Stem Cells

et al. 2006

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“…7 It is known that DBM endogenous biological activity is decreased or diminished by heating, 8 chemicals, 9 and radiation. 10 Heat, including autoclaving, not only inactivates the growth factors, but also denatures the supporting collagen structure, and activity can be lost completely after extended heating. 11 It is also known that ethylene oxide, a class I carcinogen, not only reduce bone graft osteoinductivity 12 but also may impose serious health hazards to workers, in addition to being a tedious and time-consuming process.…”

Section: Introductionmentioning

confidence: 99%

Effects of gamma irradiation on osteoinduction associated with demineralized bone matrix

Han

Yang

Nimni

2007

Journal Orthopaedic Research

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Gamma irradiation is frequently used to sterilize implanted devices but has limitations when used on biologically active materials and composites. In this study, we have evaluated the changes of biological activity of demineralized bone matrix (DBM) in the dry state and in the presence of aqueous and non-aqueous carriers while exposed to various levels of ionizing radiation. The activity of DBM in the dry state remains relatively stable with only a small loss of activity. Composites of DBM with a carrier such as lecithin, to which no water has been added, lose activity at approximately the same rate as DBM in the anhydrous form. In composites that contain water, the loss of activity occurs even at much lower levels of radiation exposure. Gamma irradiation does not change cell attachment to the DBM matrix but has an influence on both stem cell and osteoprecursor cell proliferation rates. Because of the limitations imposed by radiation, it seems most practical to handle DBM aseptically throughout the procedures of compositing pastes, putties, or suspensions, and only if necessary exposing the inert excipients to radiation sterilization prior to mixing. ß

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“…The specific mechanism behind the osteoinductivity and osteoconductivity of calcium phosphate biomaterials is a continued topic of investigation. Some have suggested that the osteoneogenic potential of such implants is the result of absorption of native bone morphogenic protein (BMP) [14][15][16][17][18]. This absorption is thought to be directly related to the pore size generated in the TCP implants.…”

Section: Discussionmentioning

confidence: 99%