Osteoinduction by a Collagen Mineral Composite Combined With Isologous Bone Marrow in a Subcutaneous Rat Model

Abstract

In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (n=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (<0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.

Effect of a β‐TCP collagen composite bone substitute on healing of drilled bone voids in the distal femoral condyle of rabbits

“…Simultaneous resorption and bone formation in the defect occurred without toxic, immunologic, and thermal interactions with the bone. In the main, an easy intraoperative handling and a good clinical application were observed in these studies as well …”

“…In the main, an easy intraoperative handling and a good clinical application were observed in these studies as well. 17,[23][24][25][26][27][28] In human patients, ceramic composites have been applied, for example, in long bone fractures 29 or dental socket ridge preservation. 30 Given its moldable but plastic consistency, the Cerasorb Ortho Foam strips could also be useful for spinal fusions.…”

Frontiers of Hydroxyapatite Composites in Bionic Bone Tissue Engineering

“…In addition, compared with OsteoSet ® (calcium sulfate), ProOsteon ® (ceramics), and DBX ® (demineralized bone matrix), Collagraft ® has a stronger ability to induce bone remodeling and bone tissue regeneration [ 195 , 196 ]. Especially when combined with bone marrow, osteogenesis is more obvious [ 197 ]. A prospective randomized controlled clinical study of 267 patients with long bone fractures found that Collagraft ® showed the same efficacy as autogenous iliac graft in the treatment of acute long bone fractures over 6–12 months [ 198 ].…”