Osteogenin, a Bone Morphogenetic Protein, Adsorbed on Porous Hydroxyapatite Substrata, Induces Rapid Bone Differentiation in Calvarial Defects of Adult Primates

Ripamonti, Ugo; Ss, Ma; B, van den Heever; Ah, Reddi

doi:10.1097/00006534-199209000-00004

Cited by 145 publications

(51 citation statements)

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“…Bone morphogenetic proteins BMP-2, BMP-3 (osteogenin), BMP-4, BMP-5, BMP-6 (Vg-1). osteogenic protein-1 (OP-1; BMP-7), and osteogenic protein-2 (BMP-8) have been implicated in cartilage and bone formation both in vitro and in vivo (3, 15,22,23,[27][28][29][30]34,36,38,42). There is accumulating evidence that morphogenetic proteins are important regulators of many morphogenetic events during embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

Helder¹,

Özkaynak²,

Sampath³

et al. 1995

J Histochem Cytochem.

175

114

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Osteogenic protein-1 (OM; BMP7) is a member of the bone morphogenetic protein subfamily. Because members of the TGF-p superfamily have a role in tissue development, the distribution of OP1 expression in developing human embryos (5-8 gestational weeks) and fetuses (8-14 gestational weeks) and mouse (9.5-17.5 gestational days) fetuses was examined. Northern hybridization with specific OB1 probes revealed two mRNA species of 4 and 2.2 KFI. Highest levels of OP1 mRNA were found in human fetal kidney and heart between 12-14 weeks of gestation. By in situ hybridization, the OP-1 transcripts were found in various tissues, i.e., the ectodermal epithelium of the mouse fore-and hindlimbs, heart, teeth, intestinal epithelium, perichondrium, hyper-

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Section: Introductionmentioning

confidence: 99%

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

Helder¹,

Özkaynak²,

Sampath³

et al. 1995

J Histochem Cytochem.

175

114

View full text Add to dashboard Cite

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“…23 Most delivery systems are typically fabricated with porous particles, granules, or scaffolds in which the bioactive protein is either adsorbed or affixed to the surfaces of the porous material, or encapsulated within the pores. [24][25][26] A disadvantage of these systems is the rapid release rate of bioactive proteins due to a limited surface area to entrap enough proteins. This, of course, would significantly impact the osteoinductive ability of BMPs.…”

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confidence: 99%

BMP2-loaded hollow hydroxyapatite microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects

Xiong¹,

Zeng²,

Yao³

et al. 2015

IJN

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The regeneration of large bone defects is an osteoinductive, osteoconductive, and osteogenic process that often requires a bone graft for support. Limitations associated with naturally autogenic or allogenic bone grafts have demonstrated the need for synthetic substitutes. The present study investigates the feasibility of using novel hollow hydroxyapatite microspheres as an osteoconductive matrix and a carrier for controlled local delivery of bone morphogenetic protein 2 (BMP2), a potent osteogenic inducer of bone regeneration. Hollow hydroxyapatite microspheres (100±25 μm) with a core (60±18 μm) and a mesoporous shell (180±42 m 2 /g surface area) were prepared by a glass conversion technique and loaded with recombinant human BMP2 (1 μg/mg). There was a gentle burst release of BMP2 from microspheres into the surrounding phosphate-buffered saline in vitro within the initial 48 hours, and continued at a low rate for over 40 days. In comparison with hollow hydroxyapatite microspheres without BMP2 or soluble BMP2 without a carrier, BMP2-loaded hollow hydroxyapatite microspheres had a significantly enhanced capacity to reconstitute radial bone defects in rabbit, as shown by increased serum alkaline phosphatase; quick and complete new bone formation within 12 weeks; and great biomechanical flexural strength. These results indicate that BMP2-loaded hollow hydroxyapatite microspheres could be a potential new option for bone graft substitutes in bone regeneration.

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“…Such porous shells of calcium carbonate have been targeted by materials scientists as having potential significance as lightweight ceramics, catalyst supports, and robust membranes for high-temperature separation technology (34). Biomedical applications include the use of these biomineralized materials for construction of artificial bone in humans (8,30,32), scaffolding supports in tissue engineering (2, 7), complement activation enhancement (29), artificial dental root construction (6,26), and biomedical implants (34). While a general understanding of some of the ecophysiological aspects of calcification and coccolithogenesis in E. huxleyi has been obtained, little information on the life cycle of this organism is available.…”

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confidence: 99%

Induction of Phase Variation Events in the Life Cycle of the Marine Coccolithophorid Emiliania huxleyi

Laguna

Romo

Read

et al. 2001

Appl Environ Microbiol

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Emiliania huxleyi is a unicellular marine alga that is considered to be the world's major producer of calcite. The life cycle of this alga is complex and is distinguished by its ability to synthesize exquisitely sculptured calcium carbonate cell coverings known as coccoliths. These structures have been targeted by materials scientists for applications relating to the chemistry of biomedical materials, robust membranes for hightemperature separation technology, lightweight ceramics, and semiconductor design. To date, however, the molecular and biochemical events controlling coccolith production have not been determined. In addition, little is known about the life cycle of E. huxleyi and the environmental and physiological signals triggering phase switching between the diploid and haploid life cycle stages. We have developed laboratory methods for inducing phase variation between the haploid (S-cell) and diploid (C-cell) life cycle stages of E. huxleyi. Plating E. huxleyi C cells on solid media was shown to induce phase switching from the C-cell to the S-cell life cycle stage, the latter of which has been maintained for over 2 years under these conditions. Pure cultures of S cells were obtained for the first time. Laboratory conditions for inducing phase switching from the haploid stage to the diploid stage were also established. Regeneration of the C-cell stage from pure cultures of S cells followed a predictable pattern involving formation of large aggregations of S cells and the subsequent production of cultures consisting predominantly of diploid C cells. These results demonstrate the ability to manipulate the life cycle of E. huxleyi under controlled laboratory conditions, providing us with powerful tools for the development of genetic techniques for analysis of coccolithogenesis and for investigating the complex life cycle of this important marine alga.

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Osteogenin, a Bone Morphogenetic Protein, Adsorbed on Porous Hydroxyapatite Substrata, Induces Rapid Bone Differentiation in Calvarial Defects of Adult Primates

Cited by 145 publications

References 0 publications

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

BMP2-loaded hollow hydroxyapatite microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects

Induction of Phase Variation Events in the Life Cycle of the Marine Coccolithophorid Emiliania huxleyi

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Osteogenin, a Bone Morphogenetic Protein, Adsorbed on Porous Hydroxyapatite Substrata, Induces Rapid Bone Differentiation in Calvarial Defects of Adult Primates

Cited by 145 publications

References 0 publications

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.

BMP2-loaded hollow hydroxyapatite&nbsp;microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects

Induction of Phase Variation Events in the Life Cycle of the Marine Coccolithophorid Emiliania huxleyi

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BMP2-loaded hollow hydroxyapatite microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects