Osteogenic potential of rat mesenchymal stem cells after several passages

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells—Implications in Tissue Engineering

“…The progressive loss of multilineage differentiation potential with in vitro passaging was also reported in previous studies [10,[13][14][15]21,[27][28][29][30]. The alkaline phosphatase activity of rat BMSCs at P1 was almost 5 times that of rat BMSCs at P3 under the osteogenic differentiation condition [28]. Banfi et al [15] reported that hBMSCs had a markedly diminished proliferation rate up to P5 and gradually lost their multiple differentiation potential up to P4.…”

Changes in chondrogenic phenotype and gene expression profiles associated with the in vitro expansion of human synovium‐derived cells

“…However, BM-MSCs lose their proliferative and differential potentials when subjected to repeated in vitro culture, a concern for their clinical applications. [15][16][17][18][19] The phenotypic changes in BM-MSCs in culture are differentiation, dedifferentiation, replicative senescence, and spontaneous transformation, and studies designed to overcome these changes have been pursued. [20][21][22][23][24] However, few studies have been undertaken on synoviumderived MSCs.…”

Enhanced bone formation in large segmental radial defects by combining adipose-derived stem cells expressing bone morphogenetic protein 2 with nHA/RHLC/PLA scaffold

“…To reduce those latent uncertainties, methods to increase cell number in scaffolds, such as retainment of seeding cells by semi-solid hydrogels [23,24] or surface modification of the scaffolds to increase cell affinity, have been attempted [25,26]. Although the reinforced ossification effects were manifest and confirmative, preparation procedures are time-consuming, accompanied by increased potential risks such as infection, loss of pluripotency, and decrease of osteogenic potential for MSCs after multiple passages [27,28].…”