Osteogenic potential of cultured human periosteum-derived cells – A pilot study of human cell transplantation into a rat calvarial defect model

Sakata, Yoshiro; Ueno, Takaaki; Kagawa, Toshimasa; Kanou, Miwa; Fujii, Takashi; Yamachika, Eiki; Sugahara, Toshio

doi:10.1016/j.jcms.2006.07.861

Cited by 50 publications

(48 citation statements)

References 16 publications

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“…Combination of both has shown spreading of human osteoblasts after eleven days in vitro (9) and new bone formation in femur (10) and rat skull (11) after 2-3 months. In a previous study, PDC-COl showed hundred times more bone in central area of rat calvaria critical-size defects (rcCsD) in comparison with the control group (5). PDC-hA implanted in subcutaneous tissue induced heterotopic ossification (4).…”

Section: Introductionmentioning

confidence: 91%

“…Disks (8 x 1.5 mm) of natural bovine xenograft (75% hA, 25% COl) were incubated in 24-well plates in the same conditions for 24 h, considering the crucial importance of protein adsorption in the initial cellular adhesion step, previously to seeding of 1.0x10 5 autogenous PDC in passage 2 onto xenograft. The medium was supplemented with 20 nM dexamethasone, 50 μg/mL ascorbic acid and 10 mM β-glycerophosphate (sigma-Aldrich Corp.), replaced every 2 days, and the set was maintained in cell culture for 6 days (2) until surgery.…”

Section: Cellular Seeding In Ha-col Scaffoldmentioning

confidence: 99%

“…Periosteum-derived cells (PDC) have osteogenic potential (2)(3)(4)(5). They are an alternative cell source in comparison with bone marrow in the tissue engineering field since they require less invasive and safer techniques by collagen (COl), biocompatible (6), biodegradable and allow adhesion, growing and cellular differentiation (7).…”

Section: Introductionmentioning

confidence: 99%

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Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Paulo

Castro-Silva²,

Oliveira

et al. 2011

Braz. Dent. J.

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The aim of this study was to evaluate the bone repair using autogenous periosteum-derived cells (PDC) and bovine anorganic apatite and collagen (hA-COl). PDC from Wistar rats (n=10) were seeded on hA-COl discs and subjected to osteoinduction during 6 days. Critical-size defects in rat calvarias were treated with blood clot (G1), autogenous bone (G2), hA-COl (G3) and hA-COl combined with PDC (G4) (n=40), and then analyzed 1 and 3 months after surgeries. radiographic analysis exhibited no significant temporal change. G1 and G2 had discrete new marginal bone, but the radiopacity of graft materials in G2, G3 and G4 impaired the detection of osteogenesis. At 3 months, histopathological analysis showed the presence of ossification islets in G1, which was more evident in G2, homogeneous new bone around hA-COl in G3 and heterogeneous new bone around hA-COl in G4 in addition to moderate presence of foreign body cells in G3 and G4. histomorphometric analysis showed no change in the volume density of xenograft (p>0.05) and bone volume density in G2 was twice greater than in G1 and G4 after 3 months (p<0.05), but similar to G3. The PDC did not increase bone formation in vivo, although the biomaterial alone showed biocompatibility and osteoconduction capacity.Key Words: critical size defects, periosteum-derived cells, xenograft, hydroxyapatite, collagen.to obtain the osteogenic cells. Also, PDC show greater proliferation, which decreases the time of cell culture, contamination risks and experiment costs. It could be a favorable alternative for dentists as a regenerative therapy for alveolar bone to treat periodontitis and dental implant dehicenses, due to the easy handling, expansion and re-implantation of cells (2).Cell culture is done upon scaffolds to simulate the 3D structure of bone tissue. Demineralized and acelullar bovine xenograft matrices are predominantly composed

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Section: Introductionmentioning

confidence: 91%

Section: Cellular Seeding In Ha-col Scaffoldmentioning

confidence: 99%

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Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Paulo

Castro-Silva²,

Oliveira

et al. 2011

Braz. Dent. J.

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“…1-3). Because of this feature, periosteal cells have been used for tissue engineering in the craniofacial region (Schmelzeisen et al, 2003;Miyamoto et al, 2004;Sakata et al, 2006). The anterior ramal surface is easy to access and has been used as a location for periosteal cell harvesting (Miyamoto et al, 2004;Sakata et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Mandibular periosteal cells have been recently used to augment the maxillary sinus floor before dental implant placement (Schmelzeisen et al, 2003) and to enhance bone repair at calvarial defects (Miyamoto et al, 2004;Sakata et al, 2006). In these studies, periosteal cells were collected either from the lateral ramal surface (Schmelzeisen et al, 2003) or from the anterior ramal surface (Miyamoto et al, 2004;Sakata et al, 2006).…”

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confidence: 99%

Molecular Variations Related to the Regional Differences in Periosteal Growth at the Mandibular Ramus

Sun

Tee

2010

The Anatomical Record

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Periosteal growth at human mandibular ramus is characterized by bone apposition at the posterior border and resorption at the anterior border. Molecular control of this regional variation is unclear. This study examined the expression of several molecules involved in bone apposition/ resorption at these regions in vivo and in vitro. By using growing pigs as a model, the periosteal growth was assessed at the mandibular ramus by vital staining and histological observations. In parallel, periosteal tissues were harvested and pulverized for RNA and protein extraction. Periosteal cells were also isolated, expanded in osteogenic media, and subjected to a single dose of dynamic tensile strain (0, 5, or 10% magnitude at 0.5 Hz) to examine their responses to mechanical loading. Real-time RT-PCR and Western blot analyses were used to examine mRNA and protein expression from periosteal tissues and cultured cells. Histological observation confirmed an anterior-resorption/posterior-apposition pattern in the pig mandibular ramus. Both in vivo tissue and in vitro cells demonstrated greater mRNA expression of receptor activator of NF-jB ligand (RANKL)/osteoprotegerin (OPG) ratio and bone morphogenetic protein 2 (BMP2) at the anterior region, while OPG expression at the anterior region was lower than the posterior region. In response to the application of a single dose of dynamic tensile strain, cultured periosteal cells appeared to change the expression profile of osteogenic markers but not that of RANKL/OPG and BMP2. These findings suggest that the unique regional variation of periosteal activity at the mandibular ramus is regulated by a differential expression of RANKL/OPG ratio (likely through differential induction of OPG) and BMP2. Anat Rec, 294:79-87, 2011. V V C 2010 Wiley-Liss, Inc.Key words: periosteum; mandibular growth; RANKL/OPG; tissue engineering; cell loadingHuman mandibular growth is mainly through endochondral bone formation at the condyles and intramembranous bone formation at the periosteum (Enlow and Hans, 1996). While condylar growth provides the overall elongation and rotation of the mandible (Bjork and Skieller, 1983), periosteal activity causes extensive surface modeling/remodeling or reshaping of the mandible (Enlow and Hans, 1996). One striking feature of mandibular periosteal activity is at the ramus, where bone resorption takes place at the anterior (rostral) border whereas bone apposition at the posterior (caudal) border (Robinson and Sarnat, 1955;Seiton and Engel, 1969;Hans et al., 1995). At the cell level, osteogenic proliferation is concentrated at the appositional posterior ramal region, whereas osteoclasts are mainly present at the resorptive anterior region (Ochareon and Herring, 2007). However, the molecular mechanisms related to these differential periosteal activities are mostly unknown.

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Engineering Vascularized Bone: Osteogenic and Proangiogenic Potential of Murine Periosteal Cells

et al. 2012

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One of the key challenges in bone tissue engineering is the timely formation of blood vessels that promote the survival of the implanted cells in the construct. Fracture healing largely depends on the presence of an intact periosteum but it is still unknown whether periosteum-derived cells (PDC) are critical for bone repair only by promoting bone formation or also by inducing neovascularization. We first established a protocol to specifically isolate murine PDC (mPDC) from long bones of adult mice. Mesenchymal stem cells were abundantly present in this cell population as more than 50% of the mPDC expressed mesenchymal markers (CD73, CD90, CD105, and stem cell antigen-1) and the cells exhibited trilineage differentiation potential (chondrogenic, osteogenic, and adipogenic). When transplanted on a collagen-calcium phosphate scaffold in vivo, mPDC attracted numerous blood vessels and formed mature bone which comprises a hematopoiesis-supportive stroma. We explored the proangiogenic properties of mPDC using in vitro culture systems and showed that mPDC promote the survival and proliferation of endothelial cells through the production of vascular endothelial growth factor. Coimplantation with endothelial cells demonstrated that mPDC can enhance vasculogenesis by adapting a pericyte-like phenotype, in addition to their ability to stimulate blood vessel ingrowth from the host. In conclusion, these findings demonstrate that periosteal cells contribute to fracture repair, not only through their strong osteogenic potential but also through their proangiogenic features and thus provide an ideal cell source for bone regeneration therapies.

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Osteogenic potential of cultured human periosteum-derived cells – A pilot study of human cell transplantation into a rat calvarial defect model

Cited by 50 publications

References 16 publications

Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Molecular Variations Related to the Regional Differences in Periosteal Growth at the Mandibular Ramus

Engineering Vascularized Bone: Osteogenic and Proangiogenic Potential of Murine Periosteal Cells

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