Osteogenic Potential of Bovine Bone Graft in Combination with Laser Photobiomodulation: An Ex Vivo Demonstrative Study in Wistar Rats by Cross-Linked Studies Based on Synchrotron Microtomography and Histology

Abstract

Background: Alveolar bone defects are usually the main concern when planning implant treatments for the appropriate oral rehabilitation of patients. To improve local conditions and achieve implant treatments, there are several methods used for increasing bone volume, among which one of the most successful, versatile, and effective is considered to be guided bone regeneration. The aim of this demonstrative study was to propose an innovative analysis protocol for the evaluation of the effect of photobiomodulation on the bone regeneration process, using rat calvarial defects of 5 mm in diameter, filled with xenograft, covered with collagen membrane, and then exposed to laser radiation. Methods: The animals were sacrificed at different points in time (i.e., after 14, 21, and 30 days). Samples of identical dimensions were harvested in order to compare the results obtained after different periods of healing. The analysis was performed by cross-linking the information obtained using histology and high-resolution synchrotron-based tomography on the same samples. A comparison was made with both the negative control (NC) group (with a bone defect which was left for spontaneous healing), and the positive control (PC) group (in which the bone defects were filled with xenografts and collagen membrane without receiving laser treatment). Results: We demonstrated that using photobiomodulation provides a better healing effect than when receiving only the support of the biomaterial. This effect has been evident for short times treatments, i.e., during the first 14 days after surgery. Conclusion: The proposed analysis protocol was effective in detecting the presence of higher quantities of bone volumes under remodeling after photobiomodulation with respect to the exclusive bone regeneration guided by the xenograft.

Biphasic Calcium Phosphate Biomaterials: Stem Cell-Derived Osteoinduction or In Vivo Osteoconduction? Novel Insights in Maxillary Sinus Augmentation by Advanced Imaging

“…Several authors agree that bone substitutes, in which thickness exceeds 400 μm, need to be vascularized in vitro before grafting them in vivo to achieve cellular survival [ 36 ]. Moreover, it was demonstrated both in vitro and in vivo that the healing process is normally delayed in the presence of biomaterials seeded with stem cells with respect to the cases of the same biomaterial but seeded with differentiated cells, most likely because stem cells growing onto the scaffold have not only to adhere and proliferate like differentiated cells but also to activate the differentiation process and all these processes need more time [ 27 , 28 , 36 , 37 , 38 , 39 ].…”

Complementarity of Photo-Biomodulation, Surgical Treatment, and Antibiotherapy for Medication-Related Osteonecrosis of the Jaws (MRONJ)

“…Photo-biomodulation (PBM) has been applied in many fields of medicine for its analgesic and anti-inflammatory effect [ 34 ]. It is also a tissue healing and repair accelerator [ 35 , 36 , 37 , 38 ]. The increased secretion of growth factors and the faster differentiation of stem cells promoted by PBM during bone regeneration have been already proven [ 39 , 40 , 41 ].…”

Micro-CT and Microscopy Study of Internal and Marginal Gap to Tooth Surface of Crenelated versus Conventional Dental Indirect Veneers

“…The latter must also be used for a porosity analysis, which is necessary to assess avoiding leakages, micro-infiltration, and fissures within the cement layer. Such micro-CT investigations come in the context of their use for a range of studies, including dental adhesives [ 28 ] or bone regeneration [ 29 , 30 ], coupled with other investigation methods, for example, microscopy [ 28 ] or optical coherence tomography (OCT) [ 31 ].…”