Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells

Wiens, Matthias; Wang, Xiaohong; Schloßmacher, Ute; Lieberwirth, Ingo; Glaßer, Gunnar; Ushijima, Hiroshi; Schröder, Heinz C.; Müller, Wernér E.G.

doi:10.1007/s00223-010-9408-6

Cited by 114 publications

(111 citation statements)

References 70 publications

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“…The technical details for the qRT-PCR have been given earlier (Wiens et al, 2010). The following two genes were selected to determine the activation state of the human chondrocytes; SOX9 SRYbox 9 (NM_000346) with the primer pair Fwd: 5′-TGCTGCTGGGAAACATTTGCAC-3′ (nt 2762 to nt 2783 ) and Rev: 5′-GGGCACTTATTGGCTGCTGAAAC-3′ (nt 2901 to nt 2879 ; 140 bp) and second COL3A1 (collagen type III alpha 1; NM_000090) with the primer pair Fwd: 5′-ATTCCTTCGACTTCTCTCCAGCC-3′ (nt 4182 to nt 4204 ) and Rev: 5′-GTGTTTCGTGCAACCATCCTCC-3′ (nt 4377 to nt 4356 ; 196 bp).…”

Section: Gene Expression Studiesmentioning

confidence: 99%

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Wang¹,

Ackermann²,

Tolba³

et al. 2016

eCM

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Here we show that inorganic polyphosphate (polyP), a polyanionic metabolic regulator consisting of multiple phosphate residues linked by energy-rich phosphoanhydride bonds, is present in the synovial fluid. In a biomimetic approach, to enhance cartilage synthesis and regeneration, we prepared amorphous polyP microparticles with Mg 2+ as counterions. The particles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) and Fourier transformed infrared spectroscopic (FTIR) analyses. Similar particles were obtained after addition of Mg 2+ ions to a solution containing hyaluronic acid, as a major component of the synovial fluid, and soluble NapolyP. The viscous paste-like material formed, composed of globular microparticles with diameter of 400 nm, strongly promoted the adhesion of chondrocytes and caused a significant upregulation of the expression of the genes encoding collagen type 3A1, as a marker for chondrocyte differentiation, and SOX9, a transcription factor that regulates chondrocyte differentiation and proliferation. The expression level of the collagen type 3A1 gene was also enhanced by exposure of chondrocytes to synovial fluid that was found to contain polyP with a size of about 80 phosphate residues. This stimulatory effect was abolished after pre-incubation of the synovial fluid with the polyP degrading alkaline phosphatase. We propose a strategy for treatment of joint dysfunctions caused by osteoarthritis based on the application of amorphous Mg 2+ -polyP microparticles that prevent calcium crystal formation in the synovial fluid using scavenging Ca -polyP to hyaluronic acid at the cartilage surface.

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Section: Gene Expression Studiesmentioning

confidence: 99%

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Wang¹,

Ackermann²,

Tolba³

et al. 2016

eCM

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“…The cultures were started with 3×10 4 cells/well in a total volume of 3 ml. After an initial incubation period of 3 days, the cultures were continued to be incubated for a total of 5 days in the absence or presence of the MAC, comprising 5 mM β-glycerophosphate, 50 mM ascorbic acid and 10 nM dexamethasone to induce biomineralization (Wiens et al, 2010b). The polyP sample, aCa-polyP-NP, was added to the cultures at concentrations between 0 and 30 µg/mL, as indicated.…”

Section: Cultivation Of Saos-2 Cellsmentioning

confidence: 99%

“…In addition, the existence of endopolyphosphatases has been analytically proven, but they have not yet been purified (Müller et al, 2015a). The released orthophosphate can act, similar to inorganic phosphate, as a source for the mineralization process of cells in culture (Wiens et al, 2010b;Müller et al, 2011); likewise, the orthophosphate can inhibit carbonic anhydrase, the seed-forming enzyme during bone mineral deposition in vitro (Müller et al, 2013). Inorganic phosphate can replace β-glycerophosphate in the mineralization-activation cocktail (MAC, usually comprising β-glycerophosphate, ascorbic acid and dexamethasone) in vitro (Müller et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Amorphous Ca2+ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells

Müller

Tolba

Feng

et al. 2015

Journal of Cell Science

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Polyphosphate ( polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca 2+ , that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units that are linked together through highenergy phosphoanhydride bonds, we questioned whether the observed morphogenetic effect, elicited by polyP, is correlated with the energy-generating machinery within the cells. We show that exposure of SaOS-2 osteoblast-like cells to polyP results in a strong accumulation of mitochondria and a parallel translocation of the polyP-degrading enzyme alkaline phosphatase to the cell surface. If SaOS-2 cells are activated by the mineralization activation cocktail (comprising β-glycerophosphate, ascorbic acid and dexamethasone) and additionally incubated with polyP, a tenfold intracellular increase of the ATP level occurs. Even more, in those cells, an intensified release of ATP into the extracellular space is also seen. We propose and conclude that polyP acts as metabolic fuel after the hydrolytic cleavage of the phosphoanhydride linkages, which contributes to hydroxyapatite formation on the plasma membranes of osteoblasts.

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“…6 Orthosilicic acid (Si[OH] 4 ), a soluble form of silicon, does not affect the synthesis of type I collagen for bone tissue regeneration, whereas an Silicon nitride is resistant to bacterial infection during bone regeneration, 9 and silicon increases the proliferation and osteogenic differentiation of osteoblast-like cells. 10 In addition, silica-based bioglass ceramics and biosilicates enhance bone-like structure formation.…”

Section: Introductionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

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Background Silicon dioxide composites have been found to enhance the mechanical properties of scaffolds and to support growth of human adipose tissue-derived stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) exists as differently sized particles when suspended in culture medium, but it is not clear whether particle size influences the beneficial effect of silicon dioxide on hADSCs. In this study, we examined the effect of different sized particles on growth and mitogen-activated protein kinase signaling in hADSCs. Methods Silica gel was prepared by a chemical reaction using hydrochloric acid and sodium silicate, washed, sterilized, and suspended in serum-free culture medium for 48 hours, and then sequentially filtered through a 0.22 μm filter (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were incubated with silica NPs or 3 μm silica microparticles (MPs), examined by transmission electron microscopy, and assayed for cell proliferation, apoptosis, and mitogen-activated protein kinase signaling. Results Eighty-nine percent of the silica NPs were around 50–120 nm in size. When hADSCs were treated with the study particles, silica NPs were observed in endocytosed vacuoles in the cytosol of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the proliferation of hADSCs, but silica MPs had no significant effect in this regard. Instead, silica MPs induced slight apoptosis. Silica NPs increased phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the ERK1/2 phosphorylation and proliferation induced by silica NPs. Conclusion Scaffolds containing silicon dioxide for tissue engineering may enhance cell growth through ERK1/2 activation only when NPs around 50–120 nm in size are included, and single component silica-derived NPs could be useful for bioscaffolds in stem cell therapy.

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Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells

Cited by 114 publications

References 70 publications

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Amorphous Ca2+ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

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