Osteogenic Potential of a Polyethylene Glycol Hydrogel Functionalized with Poly-Lysine Dendrigrafts (DGL) for Bone Regeneration

Roumani, Sandra; Jeanneau, Charlotte; Giraud, Thomas; Cotten, Aurélie; Laucournet, Marc; Sohier, Jérôme; Pithioux, Martine; About, Imad

doi:10.3390/ma16020862

Cited by 3 publications

(3 citation statements)

References 53 publications

(61 reference statements)

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“…Roumani et al conducted a study in which they developed hydrogels based on L-lysine dendrimer graft (DGL) and PEG with adjustable polymerization of reverse porosity. This modified hydrogel demonstrated the ability to enhance the interaction between endothelial cells and hMSCs, thereby increasing osteogenic potential [ 59 ]. Synthetic polymer hydrogels show great promise as carriers for delivering active proteins, growth factors, and drugs to bone tissue.…”

Section: Hydrogel Materialsmentioning

confidence: 99%

Repair of Infected Bone Defects with Hydrogel Materials

Cao,

Qin,

Duns

et al. 2024

Polymers

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Infected bone defects represent a common clinical condition involving bone tissue, often necessitating surgical intervention and antibiotic therapy. However, conventional treatment methods face obstacles such as antibiotic resistance and susceptibility to postoperative infections. Hydrogels show great potential for application in the field of tissue engineering due to their advantageous biocompatibility, unique mechanical properties, exceptional processability, and degradability. Recent interest has surged in employing hydrogels as a novel therapeutic intervention for infected bone repair. This article aims to comprehensively review the existing literature on the anti-microbial and osteogenic approaches utilized by hydrogels in repairing infected bones, encompassing their fabrication techniques, biocompatibility, antimicrobial efficacy, and biological activities. Additionally, the potential opportunities and obstacles in their practical implementation will be explored. Lastly, the limitations presently encountered and the prospective avenues for further investigation in the realm of hydrogel materials for the management of infected bone defects will be deliberated. This review provides a theoretical foundation and advanced design strategies for the application of hydrogel materials in the treatment of infected bone defects.

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Section: Hydrogel Materialsmentioning

confidence: 99%

Repair of Infected Bone Defects with Hydrogel Materials

Cao,

Qin,

Duns

et al. 2024

Polymers

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“…Testing by murine line L929 fibroblast cells revealed that they may be readily encapsulated in the gel matrix [ 142 ]. A very recent study published in 2023 also describes microspheres of hydrogels from Poly(L-lysine) dendrigrafts and PEG-bis(N-succinimidyl succinate) that can enhance osteogenic differentiation of Bone Marrow Mesenchymal Stem Cells [ 143 ].…”

Section: Dendritic Polymers Mediating To Cell Adhesion Proliferation ...mentioning

confidence: 99%

Dendritic Polymers in Tissue Engineering: Contributions of PAMAM, PPI PEG and PEI to Injury Restoration and Bioactive Scaffold Evolution

et al. 2023

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The capability of radially polymerized bio-dendrimers and hyperbranched polymers for medical applications is well established. Perhaps the most important implementations are those that involve interactions with the regenerative mechanisms of cells. In general, they are non-toxic or exhibit very low toxicity. Thus, they allow unhindered and, in many cases, faster cell proliferation, a property that renders them ideal materials for tissue engineering scaffolds. Their resemblance to proteins permits the synthesis of derivatives that mimic collagen and elastin or are capable of biomimetic hydroxy apatite production. Due to their distinctive architecture (core, internal branches, terminal groups), dendritic polymers may play many roles. The internal cavities may host cell differentiation genes and antimicrobial protection drugs. Suitable terminal groups may modify the surface chemistry of cells and modulate the external membrane charge promoting cell adhesion and tissue assembly. They may also induce polymer cross-linking for healing implementation in the eyes, skin, and internal organ wounds. The review highlights all the different categories of hard and soft tissues that may be remediated with their contribution. The reader will also be exposed to the incorporation of methods for establishment of biomaterials, functionalization strategies, and the synthetic paths for organizing assemblies from biocompatible building blocks and natural metabolites.

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“…To further promote bone regeneration, poly-L-lysine (PLL) and fibronectin (FN), which are constituents of the extracellular matrix (ECM), have been shown to improve osteoblast adhesion within the scaffold, showing osteoconductive properties [20][21][22][23], and to promote cell differentiation and the interaction of scaffolds with mesenchymal stem cells, resulting in an enhanced osteogenic potential [22,24]. PLL and FN represent promising molecules for tissue engineering [25,26], to be better explored in composite scaffolds intended for the bone regeneration of critical-size defects.…”

Section: Introductionmentioning

confidence: 99%

Polylevolysine and Fibronectin-Loaded Nano-Hydroxyapatite/PGLA/Dextran-Based Scaffolds for Improving Bone Regeneration: A Histomorphometric in Animal Study

Canciani

Straticò

Varasano

et al. 2023

IJMS

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The regeneration of large bone defects is still demanding, requiring biocompatible scaffolds, with osteoconductive and osteoinductive properties. This study aimed to assess the pre-clinical efficacy of a nano-hydroxyapatite (nano-HA)/PGLA/dextran-based scaffold loaded with Polylevolysine (PLL) and fibronectin (FN), intended for bone regeneration of a critical-size tibial defect, using an ovine model. After physicochemical characterization, the scaffolds were implanted in vivo, producing two monocortical defects on both tibiae of ten adult sheep, randomly divided into two groups to be euthanized at three and six months after surgery. The proximal left and right defects were filled, respectively, with the test scaffold (nano-HA/PGLA/dextran-based scaffold loaded with PLL and FN) and the control scaffold (nano-HA/PGLA/dextran-based scaffold not loaded with PLL and FN); the distal defects were considered negative control sites, not receiving any scaffold. Histological and histomorphometric analyses were performed to quantify the bone ingrowth and residual material 3 and 6 months after surgery. In both scaffolds, the morphological analyses, at the SEM, revealed the presence of submicrometric crystals on the surfaces and within the scaffolds, while optical microscopy showed a macroscopic 3D porous architecture. XRD confirmed the presence of nano-HA with a high level of crystallinity degree. At the histological and histomorphometric evaluation, new bone formation and residual biomaterial were detectable inside the defects 3 months after intervention, without differences between the scaffolds. At 6 months, the regenerated bone was significantly higher in the defects filled with the test scaffold (loaded with PLL and FN) than in those filled with the control scaffold, while the residual material was higher in correspondence to the control scaffold. Nano-HA/PGLA/dextran-based scaffolds loaded with PLL and FN appear promising in promoting bone regeneration in critical-size defects, showing balanced regenerative and resorbable properties to support new bone deposition.

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Osteogenic Potential of a Polyethylene Glycol Hydrogel Functionalized with Poly-Lysine Dendrigrafts (DGL) for Bone Regeneration

Cited by 3 publications

References 53 publications

Repair of Infected Bone Defects with Hydrogel Materials

Repair of Infected Bone Defects with Hydrogel Materials

Dendritic Polymers in Tissue Engineering: Contributions of PAMAM, PPI PEG and PEI to Injury Restoration and Bioactive Scaffold Evolution

Polylevolysine and Fibronectin-Loaded Nano-Hydroxyapatite/PGLA/Dextran-Based Scaffolds for Improving Bone Regeneration: A Histomorphometric in Animal Study

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