Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro

Wang, Baixiang; Yang, Jiakang; Fan, Lijie; Wang, Yu; Zhang, Chenqiu; Wang, Huiming

doi:10.1631/jzus.b2000628

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…Alizarin red combines with calcium ions to form an orange-red substance, and the calcium ion will be separated from the orange-red substance by 10% cetylpyridine, the concentration of which can be detected by determining the absorbance at 562 nm. [24] In the present study, osteoblastic differentiation was induced in MC3T3-E1 cells under the incubation with OM and was identified by the elevated production of ALP, OCN, OPN, and Col1a1 and the increased intensity of Alizarin Red S staining, in accordance with the observation in OM-treated MC3T3-E1 cells in Wang's et al [25] report. In the presence of OM, DHC was found to drastically further facilitate the secretion of osteoblastic differentiation-related proteins and enhance the intensity of Alizarin Red S staining, implying an accelerative effect of DHC on osteogenic differentiation in osteoblast precursor cells.…”

Section: Discussionsupporting

confidence: 90%

Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX‐2 signaling

Wu,

Bai,

Cai

et al. 2023

J Biochem & Molecular Tox

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Dysregulation of osteoblastic differentiation is an important risk factor of osteoporosis, the therapy of which is challenging. Dehydrocostus lactone (DHC), a sesquiterpene isolated from medicinal plants, has displayed anti‐inflammatory and antitumor properties. In this study, we investigated the effects of DHC on osteoblastic differentiation and mineralization of MC3T3‐E1 cells. Interestingly, we found that DHC increased the expression of marker genes of osteoblastic differentiation, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). Additionally, DHC increased the expressions of collagen type I alpha 1 (Col1a1) and collagen type I alpha 2 (Col1a2). We also demonstrate that DHC increased ALP activity. Importantly, the Alizarin Red S staining assay revealed that DHC enhanced osteoblastic differentiation of MC3T3‐E1 cells. Mechanistically, it is shown that DHC increased the expression of Runx‐2, a central regulator of osteoblastic differentiation. Treatment with DHC also increased the levels of phosphorylated p38, and its blockage using its specific inhibitor SB203580 abolished the effects of DHC on runt‐related transcription factor 2 (Runx‐2) expression and osteoblastic differentiation, suggesting the involvement of p38. Based on these findings, we concluded that DHC might possess a capacity for the treatment of osteoporosis by promoting osteoblastic differentiation.

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Section: Discussionsupporting

confidence: 90%

Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX‐2 signaling

Wu,

Bai,

Cai

et al. 2023

J Biochem & Molecular Tox

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“…We performed MTT assay to select the optimal serum concentration and detect cell viability. As described previously, MTT solution (5 mg/mL) was added into each well and incubated for 4 h at 37°C [ 19 ]. The supernatant was removed, and DMSO was added to dissolve the intracellular crystalline formazan product.…”

Section: Methodsmentioning

confidence: 99%

Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication

Tang

Chen

et al. 2023

Oxidative Medicine and Cellular Longevity

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Osteoblast (OB) and osteoclast (OC) play important roles in bone formation and bone resorption, which can communicate with each other through cytokine paracrine. Previous studies have confirmed that Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) used alone or in combination can treat osteoporosis (OP) through regulating bone remodeling, but the effects of EF and LLF on osteoblastogenesis, osteoclastogenesis, and OB-OC communication are unclear. In this study, we investigated the direct and indirect effects of EF and LLF on OBs and OCs via monoculture and coculture (transwell) models of OBs and OCs. We found that the combination of EF and LLF (EF&LLF) could promote osteoblastogenesis and inhibit osteoclastogenesis directly and indirectly. In order to study the mechanisms of EF&LLF on indirectly regulating osteoblastogenesis and osteoclastogenesis, we detected the expression of cytokines by which OBs and OCs could communicate with each other. We found that EF&LLF could downregulate the expression of RANKL and M-CSF and the protein ratio of RANKL/OPG of OBs and Atp6v0d2 expression of OCs and upregulate the expression of OPG and TGF-β1 of OBs and the expression of TGF-β1, BMP-2, and IGF-1 of OCs, indicating that EF&LLF could regulate cytokine expressions of OBs/OCs to affect OB-OC communication. In addition, EF&LLF had a better effect on regulating cytokines of OBs and OCs than EF or LLF in single use. This study suggested that EF&LLF exhibited the effects of promoting osteoblastogenesis and inhibiting osteoclastogenesis via acting on OB-OC communication and provided some scientific evidences for EF&LLF against OP.

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“…Due to the hollow mesoporous structure and high adsorption capacity, MSNs could load multiple molecular drugs together with miR-146a to create multifunctional nanomaterial systems, which included benidipine (an antihypertensive drug) that was demonstrated to promote osteogenesis and angiogenesis. 18 , 39 …”

Section: Discussionmentioning

confidence: 99%

MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment

Yang,

Shuai,

Siow

et al. 2024

Bone Res

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Reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a challenge, as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial, MSN+miR-146a, which showed synergistic promoting effects on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). In addition, miR-146a exhibited an anti-inflammatory effect on mouse bone marrow-derived macrophages (BMMs) under lipopolysaccharide (LPS) stimulation by inhibiting the NF-κB pathway via targeting tumor necrosis factor receptor-associated factor 6 (TRAF6), and MSNs could simultaneously promote M2 polarization of BMMs. MiR-146a was also found to inhibit osteoclast formation. Finally, the dual osteogenic-promoting and immunoregulatory effects of MSN+miR-146a were further validated in a stimulated infected mouse mandibular bone defect model via delivery by a photocuring hydrogel. Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects.

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Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro

Cited by 8 publications

References 41 publications

Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX‐2 signaling

Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX‐2 signaling

Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication

MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment

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