Osteogenic effect of polymethyl methacrylate bone cement with surface modification of lactoferrin

Xu, Derui; Song, Wenlong; Zhang, Jun; Liu, Yanting; Lü, Yanyan; Zhang, Xuewei; Liu, Qinyi; Yuan, Tianyang; Li, Rui

doi:10.1016/j.jbiosc.2021.04.006

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…The incorporation of multi-walled carbon nanotubes (MWCNTs) into PMMA bone cement improved cytocompatibility and osseointegration [ 17 ]. The addition of lactoferrin (LF) on the surface of solidified PMMA bone cement resulted in improving the adhesion, viability, proliferation, and differentiation of preosteoblasts [ 18 ]. The cell viability, growth, and cell adhesion increased for PMMA bone cement with 25% of chitosan/GO composite bone cement [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Different Nanometals Implemented in PMMA Bone Cement on Biological and Mechanical Properties

et al. 2022

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Cemented arthroplasty is a common process to fix prostheses when a patient becomes older and his/her bone quality deteriorates. The applied cements are biocompatible, can transfer loads, and dampen vibrations, but do not provide antibacterial protection. The present work is aimed at the development of cement with antibacterial effectivity achieved with the implementation of nanoparticles of different metals. The powders of Ag, Cu with particles size in a range of 10–30 nm (Cu10) and 70–100 nm (Cu70), AgCu, and Ni were added to PMMA cement. Their influence on compression strength, wettability, and antibacterial properties of cement was assessed. The surface topography of samples was examined with biological and scanning electron microscopy. The mechanical properties were determined by compression tests. A contact angle was observed with a goniometer. The biological tests included an assessment of cytotoxicity (XTT test on human cells Saos-2 line) and bacteria viability exposure (6 months). The cements with Ag and Cu nanopowders were free of bacteria. For AgCu and Ni nanoparticles, the bacterial solution became denser over time and, after 6 months, the bacteria clustered into conglomerates, creating a biofilm. All metal powders in their native form in direct contact reduce the number of eukaryotic cells. Cell viability is the least limited by Ag and Cu particles of smaller size. All samples demonstrated hydrophobic nature in the wettability test. The mechanical strength was not significantly affected by the additions of metal powders. The nanometal particles incorporated in PMMA-based bone cement can introduce long-term resistance against bacteria, not resulting in any serious deterioration of compression strength.

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Section: Introductionmentioning

confidence: 99%

Influence of Different Nanometals Implemented in PMMA Bone Cement on Biological and Mechanical Properties

et al. 2022

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“…PMMA-based acrylic bone cement is commonly used to fix bone and metallic implants in orthopedic procedures [ 52 , 53 ], but the underlying mechanistic basis of osteogenesis remains largely unknown. This study reported a possible mechanism that PMMA@ES-induced ATF2 overexpression activates miR-335-5p to target ERK1/2, thereby promoting the osteogenic differentiation and femoral defect regeneration in BMSCs and ultimately enhancing the repair of femoral defects in rats.…”

Section: Discussionmentioning

confidence: 99%

ATF2-driven osteogenic activity of enoxaparin sodium-loaded polymethylmethacrylate bone cement in femoral defect regeneration

Ding,

Hao,

Sang

et al. 2023

J Orthop Surg Res

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Background Polymethylmethacrylate (PMMA) bone cement loaded with enoxaparin sodium (PMMA@ES) has been increasingly highlighted to affect the bone repair of bone defects, but the molecular mechanisms remain unclear. We addressed this issue by identifying possible molecular mechanisms of PMMA@ES involved in femoral defect regeneration based on bioinformatics analysis and network pharmacology analysis. Methods The upregulated genes affecting the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) were selected through bioinformatics analysis, followed by intersection with the genes of ES-induced differentiation of BMSCs identified by network pharmacology analysis. PMMA@ES was constructed. Rat primary BMSCs were isolated and cultured in vitro in the proliferation medium (PM) and osteogenic medium (OM) to measure alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and the expression of RUNX2 and OCN using gain- or loss-of-function experiments. A rat femoral bone defect model was constructed to detect the new bone formation in rats. Results ATF2 may be a key gene in differentiating BMSCs into osteoblasts. In vitro cell assays showed that PMMA@ES promoted the osteogenic differentiation of BMSCs by increasing ALP activity, extracellular matrix mineralization, and RUNX2 and OCN expression in PM and OM. In addition, ATF2 activated the transcription of miR-335-5p to target ERK1/2 and downregulate the expression of ERK1/2. PMMA@ES induced femoral defect regeneration and the repair of femoral defects in rats by regulating the ATF2/miR-335-5p/ERK1/2 axis. Conclusion The evidence provided by our study highlighted the ATF2-mediated mechanism of PMMA@ES in the facilitation of the osteogenic differentiation of BMSCs and femoral defect regeneration.

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“…The results of the in vitro studies shown in Figure 5 revealed that the LF-modified PMMA bone cement improved the cell adhesion, extension, and proliferation of mouse preosteoblast (MC3T3-E1) cells. Furthermore, ALP activity and extracellular matrix (ECM) mineralization were significantly enhanced after the modification of the surface of PMMA bone cement with LF [94]. The treatment of bone defects requires the use of materials or bone cements that possess superior mechanical properties and favorable biological characteristics.…”

Section: Pmma Bone Cementsmentioning

confidence: 99%

Poly(methyl methacrylate) in Orthopedics: Strategies, Challenges, and Prospects in Bone Tissue Engineering

Ramanathan,

Lin,

Thirumurugan

et al. 2024

Polymers

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Poly(methyl methacrylate) (PMMA) is widely used in orthopedic applications, including bone cement in total joint replacement surgery, bone fillers, and bone substitutes due to its affordability, biocompatibility, and processability. However, the bone regeneration efficiency of PMMA is limited because of its lack of bioactivity, poor osseointegration, and non-degradability. The use of bone cement also has disadvantages such as methyl methacrylate (MMA) release and high exothermic temperature during the polymerization of PMMA, which can cause thermal necrosis. To address these problems, various strategies have been adopted, such as surface modification techniques and the incorporation of various bioactive agents and biopolymers into PMMA. In this review, the physicochemical properties and synthesis methods of PMMA are discussed, with a special focus on the utilization of various PMMA composites in bone tissue engineering. Additionally, the challenges involved in incorporating PMMA into regenerative medicine are discussed with suitable research findings with the intention of providing insightful advice to support its successful clinical applications.

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Osteogenic effect of polymethyl methacrylate bone cement with surface modification of lactoferrin

Cited by 10 publications

References 37 publications

Influence of Different Nanometals Implemented in PMMA Bone Cement on Biological and Mechanical Properties

Influence of Different Nanometals Implemented in PMMA Bone Cement on Biological and Mechanical Properties

ATF2-driven osteogenic activity of enoxaparin sodium-loaded polymethylmethacrylate bone cement in femoral defect regeneration

Poly(methyl methacrylate) in Orthopedics: Strategies, Challenges, and Prospects in Bone Tissue Engineering

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