Osteogenic differentiation of pre-conditioned bone marrow mesenchymal stem cells with Nisin on modified poly-L-lactic-acid nanofibers

Sadraei, Fariba; Ghollasi, Marzieh; Khakpai, Fatemeh; Halabian, Raheleh; Tehrani, Hora Jalali

doi:10.1016/j.reth.2022.07.015

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…In addition, nisin contributes to the identical protein leakage mechanism [ 6 ]. Notably, the nisin and nisin-GO coatings not only improved antibacterial performance, but also maintained good cell viability [ 6 , 47 , 48 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Peptide Conjugated on Graphene Oxide-Containing Sulfonated Polyetheretherketone Substrate for Effective Antibacterial Activities against Staphylococcus aureus

Kumar,

Hu,

et al. 2023

Antibiotics

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In the present study, the antimicrobial peptide nisin was successfully conjugated onto the surface of sulfonated polyetheretherketone (SPEEK), which was decorated with graphene oxide (GO) to investigate its biofilm resistance and antibacterial properties. The PEEK was activated with sulfuric acid, resulting in a porous structure. The GO deposition fully covered the porous SPEEK specimen. The nisin conjugation was accomplished using the crosslinker 1–ethyl–3–(3–dimethylaminopropyl)carbodiimide (EDC) through a dip-coating method. The surface micrographs of the SPEEK-GO-nisin sample indicated that nisin formed discrete islets on the flat GO surface, allowing both the GO and nisin to perform a bactericidal effect. The developed materials were tested for bactericidal efficacy against Staphylococcus aureus (S. aureus). The SPEEK-GO-nisin sample had the highest antibacterial activity with an inhibition zone diameter of 27 mm, which was larger than those of the SPEEK-nisin (19 mm) and SPEEK-GO (10 mm) samples. Conversely, no inhibitory zone was observed for the PEEK and SPEEK samples. The surface micrographs of the bacteria-loaded SPEEK-GO-nisin sample demonstrated no bacterial adhesion and no biofilm formation. The SPEEK-nisin and SPEEK-GO samples showed some bacterial attachment, whereas the pure PEEK and SPEEK samples had abundant bacterial colonies and thick biofilm formation. These results confirmed the good biofilm resistance and antibacterial efficacy of the SPEEK-GO-nisin sample, which is promising for implantable orthopedic applications.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Peptide Conjugated on Graphene Oxide-Containing Sulfonated Polyetheretherketone Substrate for Effective Antibacterial Activities against Staphylococcus aureus

Kumar,

Hu,

et al. 2023

Antibiotics

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“…Pourhajibagher et al (2022) found that curcumin poly (Llactic acid) nanoparticles containing nisin were non-toxic to human skin cells and could accelerate wound healing. Sadraei et al (2022) found that the cell proliferation and differentiation increased in bone marrow mesenchymal stem cells pretreated with nisin on a poly-L-lactic-acid scaffold. Moreover, a great number of studies have shown that nisin has immune regulation function (Contente et al, 2023;Jia et al, 2019;Malaczewska & Kaczorek-Lukowska, 2021;Malaczewska et al, 2019;Singh et al, 2014).…”

Section: Application In Medicine Fieldmentioning

confidence: 96%

“…Sadraei et al. (2022) found that the cell proliferation and differentiation increased in bone marrow mesenchymal stem cells pretreated with nisin on a poly‐L‐lactic‐acid scaffold. Moreover, a great number of studies have shown that nisin has immune regulation function (Contente et al., 2023; Jia et al., 2019; Malaczewska & Kaczorek‐Lukowska, 2021; Malaczewska et al., 2019; Singh et al., 2014).…”

Section: Application Of Nisinmentioning

confidence: 99%

Bacteriostasis of nisin against planktonic and biofilm bacteria: Its mechanism and application

Shi,

Wen,

Zhao

et al. 2024

Journal of Food Science

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Food safety incidents caused by bacterial contamination have always been one of the public safety issues of social concern. Planktonic cells, viable but non‐culturable (VBNC) cells, and biofilm cells of bacteria can coexist in food or food processing, posing more serious challenges to public health and safety by increasing bacterial survival and difficulty in detection. As a non‐toxic, no side effect, and highly effective bacteriostatic substance, nisin has received wide attention from researchers. In this review, we summarized the species and biosynthesis of nisin, the effects of nisin alone or in combination with other treatments on planktonic and biofilm cells, and its applications in the fields of food, feed, and medicine by consulting numerous studies. Meanwhile, the mechanism of nisin on planktonic and biofilm cells was proposed based on existing researches. Nisin not only has antibacterial activity against most G+ bacteria but also exhibits a bacteriostatic effect on G− bacteria when combined with other antibacterial treatments. In addition to planktonic cells, nisin also has significant effects on bacterial cells in biofilms by changing the thickness, density, and composition of biofilms. Based on the three action processes of nisin on biofilms, we summarized the changes of bacteria in biofilms, including the causes of bacterial death and the formation of the VBNC state. We consider that research on the relationship between nisin and VBNC state should be strengthened.

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Accurate expression of neck motion signal by piezoelectric sensor data analysis

Shi,

Jia,

Zhang

et al. 2024

Chinese Chemical Letters

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Osteogenic differentiation of pre-conditioned bone marrow mesenchymal stem cells with Nisin on modified poly-L-lactic-acid nanofibers

Cited by 7 publications

References 31 publications

Antimicrobial Peptide Conjugated on Graphene Oxide-Containing Sulfonated Polyetheretherketone Substrate for Effective Antibacterial Activities against Staphylococcus aureus

Antimicrobial Peptide Conjugated on Graphene Oxide-Containing Sulfonated Polyetheretherketone Substrate for Effective Antibacterial Activities against Staphylococcus aureus

Bacteriostasis of nisin against planktonic and biofilm bacteria: Its mechanism and application

Accurate expression of neck motion signal by piezoelectric sensor data analysis

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