Osteogenic Differentiation of Bone-Marrow-Derived Stem Cells Cultured with Mixed Gelatin and Chitooligosaccharide Scaffolds

Ratanavaraporn, Juthamas; Damrongsakkul, Siriporn; Kanokpanont, Sorada; Yamamoto, Masaya; Tabata, Yasuhiko

doi:10.1163/092050610x499050

Cited by 20 publications

(13 citation statements)

References 45 publications

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“…Another study examines the effect of varying gelatin (G) and chitoolisaccharide (COS) ratio on scaffold pore size, and the effect of pore size on osteogenic differentiation. 52 Scaffolds at G/COS mixing ratios of 100:0, 70:30 and 50:50 were fabricated by freeze-drying and glutaraldehyde cross-linking. Gelatin (100:0) scaffolds had the largest pore size and most homogenous distributions and higher compressive moduli than scaffolds prepared at 70:30 and 50:50 ratios.…”

Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

766

502

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The use of bone grafts is the standard to treat skeletal fractures, or to replace and regenerate lost bone, as demonstrated by the large number of bone graft procedures performed worldwide. The most common of these is the autograft, however, its use can lead to complications such as pain, infection, scarring, blood loss, and donor-site morbidity. The alternative is allografts, but they lack the osteoactive capacity of autografts and carry the risk of carrying infectious agents or immune rejection. Other approaches, such as the bone graft substitutes, have focused on improving the efficacy of bone grafts or other scaffolds by incorporating bone progenitor cells and growth factors to stimulate cells. An ideal bone graft or scaffold should be made of biomaterials that imitate the structure and properties of natural bone ECM, include osteoprogenitor cells and provide all the necessary environmental cues found in natural bone. However, creating living tissue constructs that are structurally, functionally and mechanically comparable to the natural bone has been a challenge so far. This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.

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Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

766

502

View full text Add to dashboard Cite

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“…Silk fibroin (SF) from the silkworm Bombyx mori has been used in medical application as suture materials for decades due to its biocompatibility, biodegradability, and excellent mechanical properties . These favorable properties allow researchers to explore SF application in biomedical fields such as a scaffold for bone tissue engineering, a carrier for controlled drug release, and wound healing mat . SF properties could be enhanced by blending with other material such as gelatin, chitosan/chito‐oliogosacharide, hydroxyapatite, human bone, or bee/shellac/carnauba waxes .…”

Section: Introductionmentioning

confidence: 99%

“…These favorable properties allow researchers to explore SF application in biomedical fields such as a scaffold for bone tissue engineering, a carrier for controlled drug release, and wound healing mat . SF properties could be enhanced by blending with other material such as gelatin, chitosan/chito‐oliogosacharide, hydroxyapatite, human bone, or bee/shellac/carnauba waxes . These material systems could be achieved with EDC/NHS, glutaraldehyde, electrostatic blending, or simple coating .…”

Section: Introductionmentioning

confidence: 99%

“…SF properties could be enhanced by blending with other material such as gelatin, chitosan/chito‐oliogosacharide, hydroxyapatite, human bone, or bee/shellac/carnauba waxes . These material systems could be achieved with EDC/NHS, glutaraldehyde, electrostatic blending, or simple coating . SF also could be modified with nitrogen glow discharge plasma …”

Section: Introductionmentioning

confidence: 99%

“…Most SF studies focus on improving its biocompatibility by incorporating other bioactive molecules with SF or treating SF surface with plasma . However, there is a lack of reports to investigate the modification of SF surface stiffness and its effects on cell‐substrate interaction.…”

Section: Introductionmentioning

confidence: 99%

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Oxygen Plasma Etching of Silk Fibroin Alters Surface Stiffness: A Cell-Substrate Interaction Study

Amornsudthiwat

Damrongsakkul

2014

Plasma Process. Polym.

Self Cite

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Oxygen plasma etching was used to treat silk fibroin (SF). The plasma exposure removed a significant amount of SF but left behind most surface properties close to their original state. However, nano-indenter measurements revealed a substantial increase in surface modulus of swollen SF from 62 to 500 kPa. This allowed oxygen plasma etching as a potential tool to investigate the impact of surface stiffness on cell-substrate interaction. Mouse fibroblasts (L929) and human mesenchymal stem cells (hMSC) were employed as distinct model cases. In vitro results revealed that the increased stiffness of plasma-treated SF affected only L929 adhesion, not hMSC. L929 cell attachment and spreading were better on the stiffer surface than the untreated surface, while hMSC could spread well on all SF surfaces.

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Surface modification of Thai silk fibroin scaffolds with gelatin and chitooligosaccharide for enhanced osteogenic differentiation of bone marrow‐derived mesenchymal stem cells

Wongputtaraksa¹,

Ratanavaraporn²,

Pichyangkura³

et al. 2012

J Biomed Mater Res

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In this study, the surface modification of silk fibroin (SF) scaffolds with gelatin/chitooligosaccharide (G/COS) blends using the reaction of glutaraldehyde (GA) was established. The effects of G/COS mixing ratio (100/0, 90/10, 80/20, and 70/30) and GA crosslinking concentration (0.05, 0.10, 0.15, and 0.20 vol %) on the properties of scaffolds were investigated. At 0.10-0.20 vol % GA, all G/COS blends could be successfully conjugated on the SF scaffolds, as confirmed by the percentage of weight increased and the presence of functional groups indicating SF, G, and COS from FTIR spectra. Pore size of SF scaffolds was around 570 μm with 92% porosity, however, the G/COS-conjugated SF scaffolds showed thickened pore's wall, smaller pore size (∼184-275 μm) and less porosity (∼81%), but increased density. This modified structure subsequently improved the compressive modulus of the G/COS-conjugated SF scaffolds. In terms of biological properties, the gelatin-conjugated SF scaffolds promoted the attachment and proliferation of bone marrow-derived mesenchymal stem cells (MSC) rather than the other scaffolds. However, the G/COS-conjugated SF scaffolds, particularly at the ratio of 70/30, promoted the osteogenic differentiation of MSC comparing to the SF scaffold, as confirmed by the production of alkaline phosphatase (ALP) activity and calcium (Ca), and the deposition of calcium phosphate (CaP). It was concluded that the G/COS-conjugated SF scaffolds showed great mechanical properties due to the β-structure of silk fibroin, as well as the enhanced biological properties due to the G/COS blends.

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Osteogenic Differentiation of Bone-Marrow-Derived Stem Cells Cultured with Mixed Gelatin and Chitooligosaccharide Scaffolds

Cited by 20 publications

References 45 publications

Scaffold Design for Bone Regeneration

Scaffold Design for Bone Regeneration

Oxygen Plasma Etching of Silk Fibroin Alters Surface Stiffness: A Cell-Substrate Interaction Study

Surface modification of Thai silk fibroin scaffolds with gelatin and chitooligosaccharide for enhanced osteogenic differentiation of bone marrow‐derived mesenchymal stem cells

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