Osteogenic differentiation of bone marrow-derived mesenchymal stem cells on anodized niobium surface

Antonini, Leonardo Marasca; Menezes, Tiago Lemos; Santos, Adilar Gonçalves dos; Takimi, Antônio Shigueaki; Villarinho, Denis Jardim; Santos, Bruno Paiva dos; Camassola, Melissa; Marcuzzo, Jossano Saldanha; Malfatti, Célia de Fraga

doi:10.1007/s10856-019-6305-z

Cited by 7 publications

(5 citation statements)

References 71 publications

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“…Specifically, surface energy and hydrophilicity of the NT samples increased after the anodic oxidation process, which plays a critical role in protein adsorption . It is known that changes in surface hydrophobicity and energy can alter the adsorption of proteins and their conformation, which in turn dictate stem cell adhesion and differentiation . For instance, anodized titanium surfaces were shown to be more hydrophilic compared to their NA counterparts, which induced higher fibronectin adsorption and thus allowed higher stem cell adhesion and osteogenic differentiation. , …”

Section: Results and Discussionmentioning

confidence: 99%

“…51 It is known that changes in surface hydrophobicity and energy can alter the adsorption of proteins 57 and their conformation, 58 which in turn dictate stem cell adhesion and differentiation. 59 For instance, anodized titanium surfaces were shown to be more hydrophilic compared to their NA counterparts, which induced higher fibronectin adsorption and thus allowed higher stem cell adhesion and osteogenic differentiation. 60,61 Aside from gene expression, calcium mineralization is also considered as a significant marker for osteogenic differentiation and bone regeneration.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

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Morphology of Nanostructured Tantalum Oxide Controls Stem Cell Differentiation and Improves Corrosion Behavior

Erdogan,

Uslu,

Aydınol

et al. 2023

ACS Biomater. Sci. Eng.

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Tantalum is receiving increasing attention in the biomedical field due to its biocompatible nature and superior mechanical properties. However, the bioinert nature of tantalum still poses a challenge and limits its integration into the bone tissue. To address these issues, we fabricated nanotubular (NT), nanocoral (NC), and nanodimple morphologies on tantalum surfaces via anodization. The size of these nanofeatures was engineered to be approximately 30 nm for all anodized samples. Thus, the influence of the anodized nanostructured morphology on the chemical and biological properties of tantalum was evaluated. The NT and NC samples exhibited higher surface roughness, surface energy, and hydrophilicity compared to the nonanodized samples. In addition, the NT samples exhibited the highest corrosion resistance among all of the investigated samples. Biological experiments indicated that NT and NC samples promoted human adipose tissue-derived mesenchymal stem cell (hADMSC) spreading and proliferation up to 5 days in vitro. ALP, COL1A1, and OSC gene expressions as well as calcium mineral synthesis were upregulated on the NT and NC samples in the second and third weeks in vitro. These findings highlight the significance of nanostructured feature morphology for anodized tantalum, where the NT morphology was shown to be a potential candidate for orthopedic applications.

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Section: Results and Discussionmentioning

confidence: 99%

Section: In Vitro Experimentsmentioning

confidence: 99%

Morphology of Nanostructured Tantalum Oxide Controls Stem Cell Differentiation and Improves Corrosion Behavior

Erdogan,

Uslu,

Aydınol

et al. 2023

ACS Biomater. Sci. Eng.

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“…The Zr-14Nb-5Ta-1Mo alloy showed an improved balance of mechanical properties with low magnetic susceptibility (16.96×10 −9 m 3 kg −1 ) and Young's modulus (61 GPa) 27) , which strongly implies its potential application as implants. Although the safety or promotion of osteogenic differentiation in vitro for each additional element, niobium 36,37) , tantalum 38) , and molybdenum 39,40) , respectively, has been reported, the lack of detailed information about the cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy limits its dental and medical applications.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy with MC3T3-E1 cells

et al. 2022

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The cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy were investigated using a mouse osteoblastic cell line (MC3T3-E1) to promote the application of this newly developed alloy in dental/medical treatment. The initial cell-attached morphology was visualized by fluorescent staining, and cells cultured on the Zr alloy showed similar cell adhesion behavior to cells cultured on titanium (Ti). In our 5-day proliferation investigation, similar cell numbers were obtained with both Zr alloy and Ti. These results indicate that the cytocompatibility of Zr alloy is similar to that of Ti. In addition, the similar results in the evaluation of alkaline phosphatase (ALP) activity and staining of deposited calcium using alizarin red S with both Zr alloy and Ti indicated that the osteoconductivity of the Zr alloy is similar to that of Ti. Our results prove the good cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy, enabling its promotion for use in dental/medical applications.

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“…Thereafter, a secondary mechanism is evoked-the phosphate (i.e., (PO3) 3 ⁻, (HPO3) 2 ⁻, (H2PO3)⁻) anions in body fluid with the titanate surface Ca 2+ -forming hydrated calcium phosphate, a biomineral called hydroxy appetite as a major building block of natural bone, which is critical for an osteogenic/osteoconductive environment [18] . Additionally, doping TiO2 has provided an alternative strategy to hybridize other pro-bone elements (such as other valve metals: Zr, Nb, or Ta) into heterogeneous nanostructures rather than try and develop difficult, or costly, approaches to obtain pure species such as zirconium dioxide (ZrO2, called zirconia) [19][20][21][22][23][24] . Zirconia-based nanomaterials have been demonstrated to be osteoconductive; however, their syntheses typically require a yttrium stabilization agent and/or have not become commercially viable for large-scale production [25][26][27][28][29][30] .…”

Section: Article Infomentioning

confidence: 99%

Hydrothermal synthesis of valve metal Zr-doped titanate nanofibers for bone tissue engineering

Cole,

Tian,

Thornburgh

et al. 2023

Nano. Med. Mater.

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Investigations are underway to identify novel biomaterials to improve strategies for bone tissue engineering. Hybrid nanomaterials have emerged as a viable class of biomaterials. Here, we report a facile, economical, optimized, and well-controlled hydrothermal method for synthesizing Zr-doped potassium titanate nanofibers with high purity. Upon morphological characterization, Zr-doping did not disrupt the parent crystal structure of potassium titanate, which showed huge potential for bone tissue engineering.

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Osteogenic differentiation of bone marrow-derived mesenchymal stem cells on anodized niobium surface

Cited by 7 publications

References 71 publications

Morphology of Nanostructured Tantalum Oxide Controls Stem Cell Differentiation and Improves Corrosion Behavior

Morphology of Nanostructured Tantalum Oxide Controls Stem Cell Differentiation and Improves Corrosion Behavior

Evaluation of cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy with MC3T3-E1 cells

Hydrothermal synthesis of valve metal Zr-doped titanate nanofibers for bone tissue engineering

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