Osteogenic differentiation of adipose‐derived stem cells prompted by low‐intensity pulsed ultrasound

Yue, Yuan; Yang, Xueqin; Wei, Xiaoyuan; Chen, J.; Fu, Na; Fu, Yanli; Ba, Kai; Li, G.; Yuxia, Yao; Chaochao, Liang; Zhang, J.; Cai, Xiaoxiao; Wang, M

doi:10.1111/cpr.12035

Cited by 39 publications

(30 citation statements)

References 38 publications

(44 reference statements)

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“…PCA and heat map clustering analyses of the obtained proteomic data, suggested that the most significant differences in protein expression profiles of MSCs are due to the specific growth medium (MSCGM or OM) rather than the exposure to LIPUS. Very few studies were focused indeed on osteogenic differentiation of hMSC (Gao, Walmsley, Cooper, & Scheven, ; Hu et al, ; Yue et al, ) mediated by LIPUS treatment. In this sense, our proteomics analysis revealed the existence of differences in osteoblast commitment and differentiation potential between all experimental groups.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

Costa¹,

Carina²,

Fontana

et al. 2017

Journal Cellular Physiology

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Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7, 14, 21 days). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21 days CD73+/CD90+: 6%; OCT-3/4+/NANOG+/SOX2+: 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

Costa¹,

Carina²,

Fontana

et al. 2017

Journal Cellular Physiology

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“…Then the suspension was added into plates or T25 flasks at 37°C in a humidified atmosphere of 5% CO 2 . Purified ASCs should be subcultured to passage 2 for further experiments …”

Section: Methodsmentioning

confidence: 99%

The JAK/STAT3 signalling pathway regulated angiogenesis in an endothelial cell/adipose‐derived stromal cell co‐culture, 3D gel model

et al. 2016

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“…However, TO could cause erosion of PDL fibre and promote the entry of inflammatory cells into periodontal tissues, thereby accelerating the destruction of periodontal support tissues (Ramfjord & Ash, ; Svanberg et al., ). Our previous in vitro studies showed that mechanical stimulations affected the osteogenic ability of adipose‐derived stem cells and myelinization potential of Schwann cells (Yue et al., , ).…”

Section: Discussionmentioning

confidence: 99%

Traumatic occlusion aggravates bone loss during periodontitis and activates Hippo‐YAP pathway

Pan

Yang

et al. 2019

J Clinic Periodontology

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Aim This study aimed at exploring changes in YAP expression and their effect on periodontitis (PD) combined with traumatic occlusion (TO). Materials and Methods BALB/cJ mice were used to establish a PD model by local administration of Porphyromonas gingivalis (P.g, ATCC 33277) and a TO model by occlusal elevation (OE) using composite resin bonding on the bilateral maxillary molar. The mouse fibroblast cell line (L929) and pre‐osteoblast cell line (MC3T3‐E1) were subjected to cyclic tensile/compressive stress and inflammatory stimuli (lipopolysaccharide from Escherichia coli) to verify in vivo results. Results Severe bone resorption was observed by microCT scanning in OE with P.g group, when compared to OE only and P.g only groups. Mechanical stress caused by OE activated the Hippo‐YAP pathway in periodontal tissues and upregulated the expression of JNK/AP‐1. OE with P.g further promoted the expression of YAP and JNK/AP1, leading to the upregulation of the JNK/AP‐1 related inflammatory cytokines TNF‐α and IL6. Similar results were obtained when osteoblasts were subjected to mechanical stress in vitro. Conclusions Our study demonstrated that periodontitis with TO caused severe inflammation‐induced bone resorption. Activation of YAP and upregulation of JNK/AP‐1 induced by TO potentially aggravated the symptoms of PD.

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Osteogenic differentiation of adipose‐derived stem cells prompted by low‐intensity pulsed ultrasound

Abstract: We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.

Cited by 39 publications

References 38 publications

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

The JAK/STAT3 signalling pathway regulated angiogenesis in an endothelial cell/adipose‐derived stromal cell co‐culture, 3D gel model

Traumatic occlusion aggravates bone loss during periodontitis and activates Hippo‐YAP pathway

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