Osteogenic Commitment of MSC Is Enhanced after Interaction with Umbilical Cord Blood Mononuclear Cells In Vitro

Андреева, Е.Р.; Ездакова, М. И.; Бобылева, П. И.; Андрианова, И. В.; Ratushnyy, A. Yu.; Buravkova, Ludmila

doi:10.1007/s10517-021-05266-5

Cited by 3 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Group I factors can be considered as homeostatic ones controlling cell functions such as proliferation (G-CSF), angiogenic signaling (VEGF), niche formation (GRO, IL-8, MCP-1), cell senescence (IL-6) [38], while group II factors are likely to serve as a reaction to the local needs or challenges. Data on MSCs secretome are limited and heterogeneous [39][40][41][42]. Increased production of G-CSF, GM-CSF, IP-10, MIP-1α, and MCP-3 during osteogenesis was shown by several groups [39,40].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Svirshchevskaya

Sharonova

Полтавцева

et al. 2022

IJMS

View full text Add to dashboard Cite

Multiple studies have demonstrated that various nanoparticles (NPs) stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) and inhibit adipogenic ones. The mechanisms of these effects are not determined. The aim of this paper was to estimate Wharton’s Jelly MSCs phenotype and humoral factor production during tri-lineage differentiation per se and in the presence of silicon–gold NPs. Silicon (SiNPs), gold (AuNPs), and 10% Au-doped Si nanoparticles (SiAuNPs) were synthesized by laser ablation, characterized, and studied in MSC cultures before and during differentiation. Humoral factor production (n = 41) was analyzed by Luminex technology. NPs were nontoxic, did not induce ROS production, and stimulated G-CSF, GM-CSF, VEGF, CXCL1 (GRO) production in four day MSC cultures. During MSC differentiation, all NPs stimulated CD13 and CD90 expression in osteogenic cultures. MSC differentiation resulted in a decrease in multiple humoral factor production to day 14 of incubation. NPs did not significantly affect the production in chondrogenic cultures and stimulated it in both osteogenic and adipogenic ones. The major difference in the protein production between osteogenic and adipogenic MSC cultures in the presence of NPs was VEGF level, which was unaffected in osteogenic cells and 4–9 times increased in adipogenic ones. The effects of NPs decreased in a row AuNPs > SiAuNPs > SiNPs. Taken collectively, high expression of CD13 and CD90 by MSCs and critical level of VEGF production can, at least, partially explain the stimulatory effect of NPs on MSC osteogenic differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Increased production of G-CSF, GM-CSF, IP-10, MIP-1α, and MCP-3 during osteogenesis was shown by several groups [39,40]. However, concentrations of different factors vary significantly from paper to paper [41,42], possibly due to the different commercial kits used.…”

Section: Discussionmentioning

confidence: 99%

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Svirshchevskaya

Sharonova

Полтавцева

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

Simulation of Microgravity and Coculturing with Hematopoietic Cells Oppositely Modulate Wnt Signaling in Mesenchymal Stromal Cells

Ratushnyy

Tyrina

Буравкова

2023

Dokl Biochem Biophys

View full text Add to dashboard Cite

Simulated Microgravity and Coculturing With Hematopoietiic Cells Oppositely Modulate WNT Signaling in Mesenchymal Stromal Cells

Ratushnyy,

Tyrina,

Buravkova

2023

Doklady Rossijskoj akademii nauk. Nauki o žizni

View full text Add to dashboard Cite

The osteogenic potential of mesenchymal stromal cells (MSCs) can determine the bone homeostasis and the physical characteristics of bones. Microgravity reduces the ability of these cells to differentiate in osteogenic direction. It has been shown that the addition of hematopoietic stem and progenitor cells (HSPCs) to MSC culture in vitro can have the opposite effect. The aim of this study was to identify transcriptional changes in 84 genes associated with Wnt signaling in MSCs during microgravity simulation and interaction with HSPCs. The results indicate an increase in the non-canonical Wnt signaling activity during MSCs and HSPCs cocultivation, while simulated microgravity enhances the canonical component of this signaling pathway. These changes may underlie the modulation of osteogenic potential of MSCs in hematopoietic niche under microgravity.

show abstract

Osteogenic Commitment of MSC Is Enhanced after Interaction with Umbilical Cord Blood Mononuclear Cells In Vitro

Cited by 3 publications

References 29 publications

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Simulation of Microgravity and Coculturing with Hematopoietic Cells Oppositely Modulate Wnt Signaling in Mesenchymal Stromal Cells

Simulated Microgravity and Coculturing With Hematopoietiic Cells Oppositely Modulate WNT Signaling in Mesenchymal Stromal Cells

Contact Info

Product

Resources

About