Osteogenesis of Electrically Stimulated Bone Cells Mediated in Part by Calcium Ions

Wang, Qian; Zhang, Shizhen; Ouyang, Jun; Jiang, Lixin; Zhang, Zhengkun; Xie, Yang; Luo, Shen-qiu

doi:10.1097/00003086-199803000-00037

Cited by 67 publications

(57 citation statements)

References 19 publications

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“…The authors (Bodamyali et al, 1999) did not look at the calcium on the salt bridge but rather measured the calcium concentration in the media with a spectrophotometer by examining the calcium's reaction with o-cresolphthalein, which forms a red complex at pH 10-12 with an absorbance maximum at 575 nm. Calcium uptake by mouse calvaria was suspected due to the acceleration of the intracellular transport of calcium, as previously reported (Wang et al, 1998), and may increase the activation of the calcium/calmodulin signalling pathway (Lorich et al, 1998;Zhuang et al, 1997). While the salt bridge technique has been extensively used to study the effect of ES on cultured cells, this approach can be problematic when applied for tissue engineering purposes.…”

Section: Salt Bridgementioning

confidence: 82%

Electrical Stimulation in Tissue Regeneration

Meng¹,

Rouabhia²,

Zhang³

2011

Applied Biomedical Engineering

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Section: Salt Bridgementioning

confidence: 82%

Electrical Stimulation in Tissue Regeneration

Meng¹,

Rouabhia²,

Zhang³

2011

Applied Biomedical Engineering

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“…Some clinical studies and animal experiments have suggested enhancement of ossification by electrical stimulation [1,7,11,26,32,36,40]. In order to elucidate the mechanisms of electric-field stimulation on osteoprogenitor cells, many in vitro studies have applied direct electrical currents [8,12,29,37,41], capacitive coupling of electric fields [16,28,42] and inductive coupling of electric fields [9,19]. Ochi et al showed that the bone contact ratio around a dental implant inserted in the rabbit femur was almost 70% when capacitive coupling of electric fields (60 kHz, 10 Vp-p, sine wave signals) was applied for 8 hr/day over 14 days [32].…”

Section: Discussionmentioning

confidence: 99%

Helical Conformation Endows Poly-<span style="font-variant: small-caps;">l</span>-Lactic Acid Fibers with a Piezoelectric Charge under Tensile Stress

Harada

Kadono

Terao

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Poly-l-lactic acid (PLLA) has been clinically used as a bioabsorbable material and attains a piezoelectric charge upon molecular orientation by the application of a shear force to the C-axis of the crystal line region. Previous studies showed that implanted drawn PLLA films or rods accelerate the ossification due to piezoelectric effect. In this study, we originally designed helically-twisted PLLA fiber to produce piezoelectricity in bioabsorbable suture upon tensile stress. The piezoelectricity of the helical PLLA fibers was evaluated using a lock-in amplifier system in vitro. The ossification induced by helical PLLA fibers was examined by implanting them in the rat patellar ligament supporting a physiological tensile load. We observed that 57° and 45° twisted PLLA fibers generated a higher piezoelectric potential than did 27° twisted fibers. The animal experiment showed that the formation of osseous tissue around helical PLLA fibers was more significant than around non-helical control fibers at 4 weeks after their implantation. These results suggest that helical PLLA fiber may be useful for the surgical suture or artificial ligament, which connects to the bone. KEW WORDS: animal model, helical conformation, ossification, piezoelectricity, poly-l-lactic acid.doi: 10.1292/jvms.12-0323; J. Vet. Med. Sci. 75(9): 1187-1192, 2013 Ligament injury, such as anterior cruciate ligament rupture, is a common cause of lameness in small animal practice [6,15]. Various types of artificial ligaments including bioabsorbable material have been studied to reconstruct the injured ligament [3,27,31]. It has been suggested that early osteointegration of the grafted artificial ligament to the bone is important to improve the ligament function and durability [39].Poly-l-lactic acid (PLLA) is well known as a bioabsorbable material and has been clinically used as bioabsorbable mini-plate, screw and pin [2,13,14,30,33]. On the other hand, PLLA is known as a piezoelectric material. Fukada reported that an external mechanical stress to a drawn PLLA film gives rise to electrical polarization [10], and this piezoelectric effect is ascribed to the change in the microscopic orientation of crystals with a fundamentally asymmetrical structure [5,10,22,23]. Animal experiments showed that the piezoelectricity of drawn PLLA films and rods accelerated callus formation on bone [10,22,35]. Shimono et al. prepared three types of PLLA piezoelectric films with different oriented directions (0°, 45° or 90° to the drawing direction) and implanted them on the periosteum of the rabbit tibia [35]. These drawn PLLA films promoted ossification to a greater extent than undrawn PLLA films, and the effect of drawn PLLA films in promoting ossification was greatest when a shearing stress was applied at 45° to the axis of orientation. Ikada et al. prepared drawn PLLA rods with different draw ratios and implanted these rods intramedullary in the osteotomized tibia of cats [22]. Fracture healing was clearly promoted with increased callus formation as the draw...

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“…It was reported that the release of silicilic acid by Si-contained coatings in culture medium caused its alkalinization due to ion exchange [26] . In addition, voltage-activated calcium channels were found in the membrane of osteoblasts [27] and the alkalinization could increase the channels sensitivity and also enhance the cellular calcium entry [28] , which lead to the increased osteoblast viability and proliferation eventually. Furthermore, the released Si could bind to oxygen and form a silicate network structure on the surface, which was capable of holding elements of the proteins together in an organized fashion [29] .…”

Section: Discussionmentioning

confidence: 99%