Osteogenesis and aging: lessons from mesenchymal stem cells

Infante, Arantza; Rodrı́guez, Clara I.

doi:10.1186/s13287-018-0995-x

Cited by 231 publications

(194 citation statements)

References 59 publications

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“…We then evaluated gene expression during the different phases of hMSCs mineralization using the two experimental procedures (primed vs. chronically treated groups). As shown in Table , we evaluated osteogenic markers expressed in different stages of mineralization (early, early to mid, mid to late, late; Heo, Lee, & Kim, ; Infante & Rodriguez, ; Lambertini et al, ; Lisignoli et al, ; Manferdini et al, ; Midha, Murab, & Ghosh, ; Shekaran et al, ; Zhang et al, ). Only 3 out of 14 genes analyzed showed a significant difference between the two experimental procedures used: TNFRSF11b ( p = .005), BGLAP ( p = .016), and RUNX2 ( p = .005; data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…MSCs osteogenic differentiation is a complex process characterized by a balance between reduction of cell proliferation and induction of cell mineralization where different genes are involved during the different stages of mineralization (Infante & Rodriguez, ). In line with other reports (Frank et al, ; Midha et al, ; Shekaran et al, ) we summarized in Table , the genes analyzed and their expression in the early or mid or late stage of mineralization.…”

Section: Discussionmentioning

confidence: 99%

“…MSCs osteogenic differentiation is a process characterized by a sequence of events tightly regulated, but is still only partially understood. At the beginning, MSCs lose their proliferative capacity, acquiring the ability to respond to osteogenic stimuli, and then extra cellular matrix (ECM) support nascent osteoblasts commitment, maturation, and mineralization (Infante & Rodriguez, 2018;Manferdini et al, 2018).…”

Section: Janowskamentioning

confidence: 99%

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Specific concentration of hyaluronan amide derivative induces osteogenic mineralization of human mesenchymal stromal cells: Evidence of RUNX2 and COL1A1 genes modulation

Paolella

Gabusi

Manferdini

et al. 2019

J Biomedical Materials Res

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Hyaluronic acid (HA) is an ideal material for tissue regeneration. The aim of this study was to investigate whether a hyaluronan amide derivative (HAD) can enhance the mineralization of human mesenchymal stem cells (hMSCs). Osteogenically induced hMSCs cultured with or without HAD at different concentrations (0.5 mg/ml or 1 mg/ml) were analyzed for mineral matrix deposition, metabolic activity, cellular proliferation, and the expression of 14 osteogenic genes. Unmodified HA (HYAL) was used as control. We demonstrated that only cells treated daily until day 28 with 0.5 mg/ml HAD, but not with 1 mg/ml of HAD and HYAL, showed a significant induction of mineralization at day 14 compared to the osteogenic control group. HAD at both concentrations tested, significantly decreased the expression of the proliferating marker MKI67 at day 2. By contrast, increased metabolic activity was induced only by HYAL from day 14. HAD at both concentrations significantly down modulated SNAI2, DLX5, RUNX2, COL1A1, and IBSP genes, while significantly up regulated COL15A1. The induction of mineralization of 0.5 mg/ml of HAD at day 14 was significantly dependent on a specific modulation of RUNX2 and COL1A1. Our data demonstrate that only 0.5 mg/ml of HAD, but not HYAL, modulated hMSCs osteogenic differentiation, suggesting that the physicochemical features and concentration of HA products could differently affect osteogenic maturation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Janowskamentioning

confidence: 99%

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Specific concentration of hyaluronan amide derivative induces osteogenic mineralization of human mesenchymal stromal cells: Evidence of RUNX2 and COL1A1 genes modulation

Paolella

Gabusi

Manferdini

et al. 2019

J Biomedical Materials Res

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“…One of the important functions of BMSCs is their ability to differentiate into osteogenic cells and contribute in bone homeostasis [14]. The major hallmarks of osteogenesis are expression of osteogenic marker genes, production of ALP and deposition of calcium phosphate crystals [13, 15].…”

Section: Discussionmentioning

confidence: 99%

Chikungunya virus infection impairs the function of osteogenic cells

Roy

Shi

Duan

et al. 2020

Preprint

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19Chikungunya virus (CHIKV) is a positive-sense, single-stranded RNA virus, spread by 20 the Aedes species (sp.) mosquitoes. Chikungunya virus (CHIKV) causes a condition 21 characterized by high fever, headache, rash, and joint pain. Recent investigations 22Presently, no vaccines or treatment options are available for CHIKV infection. Joint pain 42 is one of the major concerns. Although studies have shown an association between 43 bone pathology and infection, the molecular pathogenesis in context of bone pathology 44 is poorly defined. Here, we demonstrate for the first time that BMSCs and BMSC-45 derived osteogenic cells are susceptible to CHIKV infection and infection likely alters 46 function of the osteogenic cells. This study highlights altered osteogenic differentiation 47 as a possible mechanism for causing the bone pathology observed in CHIKV 48 pathogenesis. 49Keywords 50 Chikungunya virus, mesenchymal stem cells, osteogenic differentiation 51Recent studies identified bone lesions in the joints of CHIKV infected mice, 65indicating that CHIKV can cause bone pathologies [12]. In another study, mice infected 66 with a similar arthritogenic alphavirus, Ross River virus (RRV) resulted in significant 67 bone loss [7]. In humans, magnetic resonance imaging (MRI) studies revealed that 68 CHIKV infection is associated with erosive arthritis [19]. Taken together these studies 69 suggest alphavirus infection can affect bone homeostasis and thus contribute to 70

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“…Osteoporosis is an age‐related disease characterized by cumulative changes in bone metabolism that suppress bone formation and promote bone resorption (Rachner, Khosla, & Hofbauer, ; Reginster & Burlet, ). Together, these changes contribute to disordered bone homeostasis (Infante & Rodriguez, ; Wei & Sun, ). Bone homeostasis relies on a dynamic balance between osteogenesis by osteoblasts and osteoclastogenesis by osteoclasts (Cummings & Melton, ).…”

Section: Introductionmentioning

confidence: 99%

Deterioration of hematopoietic autophagy is linked to osteoporosis

et al. 2020

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Hematopoietic disorders are known to increase the risk of complications such as osteoporosis. However, a direct link between hematopoietic cellular disorders and osteoporosis has been elusive. Here, we demonstrate that the deterioration of hematopoietic autophagy is coupled with osteoporosis in humans. With a conditional mouse model in which autophagy in the hematopoietic system is disrupted by deletion of the Atg7 gene, we show that incapacitating hematopoietic autophagy causes bone loss and perturbs osteocyte homeostasis. Induction of osteoporosis, either by ovariectomy, which blocks estrogen secretion, or by injection of ferric ammonium citrate to induce iron overload, causes dysfunction in the hematopoietic stem and progenitor cells (HSPCs) similar to that found in autophagy-defective mice.Transcriptomic analysis of HSPCs suggests promotion of iron activity and inhibition of osteocyte differentiation and calcium metabolism by hematopoietic autophagy defect, while proteomic profiling of bone tissue proteins indicates disturbance of the extracellular matrix pathway that includes collagen family members. Finally, screening for expression of selected genes and an immunohistological assay identifies severe impairments in H vessels in the bone tissue, which results in disconnection of osteocytes from hematopoietic cells in the autophagy-defective mice. We therefore propose that hematopoietic autophagy is required for the integrity of H vessels that bridge blood and bone cells and that its deterioration leads to osteoporosis. SPSS 25.0. Experimental data were presented as means ± standard deviations (SDs), which was evaluated using Student's t tests.University, for their help in preparing human bone samples and creating mouse osteoporotic model, and Jin Qi from the Shanghai

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Osteogenesis and aging: lessons from mesenchymal stem cells

Cited by 231 publications

References 59 publications

Specific concentration of hyaluronan amide derivative induces osteogenic mineralization of human mesenchymal stromal cells: Evidence of RUNX2 and COL1A1 genes modulation

Specific concentration of hyaluronan amide derivative induces osteogenic mineralization of human mesenchymal stromal cells: Evidence of RUNX2 and COL1A1 genes modulation

Chikungunya virus infection impairs the function of osteogenic cells

Deterioration of hematopoietic autophagy is linked to osteoporosis

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