Osteodifferentiation of Human Preadipocytes Induced by Strontium Released from Hydrogels

Abstract

In recent years, there has been an increasing interest in interactive application principles of biology and engineering for the development of valid biological systems for tissue regeneration, such as for the treatment of bone fractures or skeletal defects. The application of stem cells together with biomaterials releasing bioactive factors promotes the formation of bone tissue by inducing proliferation and/or cell differentiation. In this study, we used a clonal cell line from human adipose tissue-derived mesenchymal stem cells (hADSCs or preadipocytes), named PA2-E12, to evaluate the effects of strontium (Sr2+) released in the culture medium from an amidated carboxymethylcellulose (CMCA) hydrogel enriched with different Sr2+ concentrations on osteodifferentiation. The osteoinductive effect was evaluated through both the expression of alkaline phophatase (ALP) activity and the hydroxyapatite (HA) production during 42 days of induction. Present data have shown that Sr2+ released from CMCA promotes the osteodifferentiation induced by an osteogenic medium as shown by the increase of ALP activity at 7 and 14 days and of HA production at 14 days. In conclusion, the use of biomaterials able to release in situ osteoinductive agents, like Sr2+, could represent a new strategy for future applications in bone tissue engineering.

Effects of Sr-HT-Gahnite on osteogenesis and angiogenesis by adipose derived stem cells for critical-sized calvarial defect repair

“…We assumed that the much higher concentration of Ca and Si ion released from Sr-HT-Gahnite attributed to its better osteogenic activity both in vitro and in vivo . The treatment of Sr ion could not only enhance the cell proliferation and osteogenic differentiation of ASCs in vitro 33, but also accelerate new bone matrix formation in vivo 34.…”

Adipose mesenchymal stem cells in the field of bone tissue engineering

“…Many types of materials have been used to confirm these positive hASC characteristics, which have become available for scaffoldassisted bone regeneration in a variety of tissue engineering strategies. The importance of the scaffold in hASC osteogenesis has been demonstrated in a number of studies that recommend the use of different materials, including ceramics [73] , titan alloys [74,75] , natural and synthetic polymers [76,77] , and natural or semi-synthetic grafts [78,79] , with variable porosity, roughness, and methods of fabrication for future regenerative applications. A clear trend has emerged toward the use of composite scaffolds due to their superior properties and structures [80][81][82] derived from the combination of two or more materials [83][84][85][86][87] .…”

Tailor‐made multicomponent electrospun polyurethane nanofibrous composite scaffold comprising olive oil, honey, and propolis for bone tissue engineering

“…The commercial approaches used for reconstruction of the bone tissue structure were autografts or allografts. However, the use of autografts and allografts were limited owing to the risk of immunogenicity and infection . Nowadays, the tissue engineered approaches have been reported to hold a great promise for biomedical applications.…”