Osteocyte lacunar strain determination using multiscale finite element analysis

Kola, Sravan Kumar Reddy; Begonia, Mark T.; Tiede-Lewis, LeAnn M.; Laughrey, Loretta E.; Dallas, Sarah L.; Johnson, Mark; Thiagarajan, Ganesh

doi:10.1016/j.bonr.2020.100277

Cited by 16 publications

(4 citation statements)

References 40 publications

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“…Our findings may also explain the preferential orientation of the osteocyte long axes in parallel to loading direction (Aviral Vatsa et al, 2008), minimizing the effects of stress concentrating and crack initiation (Currey, 2003;CURREY, 1962;Nicolella et al, 2006). Our data are in agreement with the results found recently by Kola et al (Kola et al, 2020) who demonstrated lower lacunar strains for the osteocytes aligned along the loading axis compared to osteocytes aligned perpendicular to loading axis and higher strains for osteocytes with larger lacunae volume. Furthermore, the maximum EFF strains in ECM significantly decreased with increasing distance from the lacuna boundary whereas the minimum EFF strains significantly increased with increasing distance from the lacuna boundary in both young and old fibulae.…”

Section: Discussionsupporting

confidence: 93%

Alterations in osteocyte lacunar morphology affect local bone tissue strains

Hemmatian

Bakker

Klein‐Nulend

et al. 2021

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionsupporting

confidence: 93%

Alterations in osteocyte lacunar morphology affect local bone tissue strains

Hemmatian

Bakker

Klein‐Nulend

et al. 2021

Journal of the Mechanical Behavior of Biomedical Materials

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“…Additionally, load:strain relationship studies ( Mumtaz et al, 2020b ) could be performed to determine if this relationship is significantly altered in the OVX mice. At present, the exact strain experienced by each osteocyte on the compression or tensile side of the bone is unknown but could be modeled by finite element analysis ( Stern et al, 2018 ; Kola et al, 2020 ). Another important caveat to our biomechanical testing and microCT analysis is that those properties were measured in the femur and the loading occurred in the ulna.…”

Section: Discussionmentioning

confidence: 99%

Osteocyte Wnt/β-catenin pathway activation upon mechanical loading is altered in ovariectomized mice

et al. 2021

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Estrogen levels decline in both sexes with age, but more dramatically in females. Activation of the Wnt/β-catenin signaling pathway is central to the regulation of bone mass accrual and maintenance and in response to mechanical loading. Using the ovariectomized mouse model we examined the effect of estrogen loss on the osteocyte's ability to activate the Wnt/β-catenin pathway following mechanical loading. Female TOPGAL mice underwent ovariectomy (OVX) ( n = 10) or sham surgery ( n = 10) at 16 weeks of age. Four weeks post-surgery, a single loading session (global strain of 2200 με for 100 cycles at 2 Hz) was performed on the right forearm with the left as a non-loaded control. Mice ( n = 5) were sacrificed at 1 or 24 hr post-load. Ulnae were stained for β-catenin activation, femurs were used for μCT and 3-pt bending/biomechanical testing, and tibiae were used for histology analysis and to determine osteocyte lacunar size using SEM and high resolution micro-XCT. A 2.2-fold increase in β-catenin signaling activation was observed 24 hr post-load in the Sham group but did not occur in the OVX group. The OVX group versus control had significant losses ( p < 0.05) in trabecular BMD (−8%), BV/TV (−35%) and thickness (−23%), along with cortical thickness (−6%) and periosteal perimeter (−4%). The OVX group had significantly higher trabecular bone osteoclast numbers (63%), OCS/BS (77%) and N.OC/BPm (94%) and a significant decrease in osteoblast number (53%), OBS/BS (37%) and N.OB/BPm (40%) compared to the sham group ( p < 0.05). Cortical bone lacunar number/lacunar volume and bone biomechanical properties did not change between groups. Given that the ulna is a cortical bone loading model and the lack of changes in osteocyte lacunar number/volume in cortical bone, which would alter strains experienced by osteocytes, these data suggest the absence of estrogen resulted in intrinsic changes in the ability of the osteocyte to respond to mechanical load, rather than changes in the biomechanical and architectural properties of bone.

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“…Despite the mechanism of stimuli perception in osteocytes not being fully understood, both cell bodies (e.g., osteocytes plasma membrane disruptions) [61] and dendritic processes [62,63] have been proven to perceive mechanical forces applied to the bone. Kola et al [55] demonstrated that lacunar strain value increased as lacunae size increased, with the highest strain magnification ratio observed for horizontally aligned lacunae. The authors also demonstrated the influence of the perilacunar region modulus in the strain magnification generated at the lacuna.…”

Section: Microstructurementioning

confidence: 99%

“…An osteocyte is made up of an ellipsoid cell body that occupies a fluid-filled space within its lacunae. Osteocytes are interconnected by many long dendritic processes that pass through a network of small channels called canaliculi (as can be seen in Figure 2) [54,55]. The process of converting external mechanical forces into biochemical responses, known as mechanotransduction [5], includes the response of the osteocyte to the cell direct mechanical deformation/strain as a consequence of bone matrix strain [56], to shear stress due to load-induced fluid flow [57], to electric fields caused by stress-generated streaming potentials [58], and to hydrostatic pressure [59].…”

Section: Microstructurementioning

confidence: 99%

Bone: An Outstanding Composite Material

et al. 2022

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Bone is an outstanding, well-designed composite. It is constituted by a multi-level structure wherein its properties and behavior are dependent on its composition and structural organization at different length scales. The combination of unique mechanical properties with adaptive and self-healing abilities makes bone an innovative model for the future design of synthetic biomimetic composites with improved performance in bone repair and regeneration. However, the relation between structure and properties in bone is very complex. In this review article, we intend to describe the hierarchical organization of bone on progressively greater scales and present the basic concepts that are fundamental to understanding the arrangement-based mechanical properties at each length scale and their influence on bone’s overall structural behavior. The need for a better understanding of bone’s intricate composite structure is also highlighted.

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Osteocyte lacunar strain determination using multiscale finite element analysis

Cited by 16 publications

References 40 publications

Alterations in osteocyte lacunar morphology affect local bone tissue strains

Alterations in osteocyte lacunar morphology affect local bone tissue strains

Osteocyte Wnt/β-catenin pathway activation upon mechanical loading is altered in ovariectomized mice

Bone: An Outstanding Composite Material

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