Osteocyte lacunae in transiliac bone biopsy samples across life span

Blouin, Stéphane; Misof, Barbara M.; Mähr, Matthias; Fratzl‐Zelman, Nadja; Roschger, Paul; Lueger, Sonja; Messmer, Phaedra; Keplinger, Petra; Rauch, Frank; Glorieux, Francis H.; Berzlanovich, Andrea; Gruber, G.M.; Brugger, Peter C.; Shane, Elizabeth; Recker, Robert R.; Zwerina, Jochen; Hartmann, Markus

doi:10.1016/j.actbio.2022.11.051

Cited by 5 publications

(9 citation statements)

References 76 publications

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“…We found lower total osteocyte lacunar density than reported in previous studies, 28 , 29 , 37 which could be explained by the differences in the bone compartment and skeletal site analyzed. Specifically, cortical bone has higher osteocyte density than trabecular bone, 28 and there is substantial heterogeneity in osteocyte lacunar density between different skeletal sites. 28 , 29 , 37 …”

Section: Discussioncontrasting

confidence: 83%

“…These include the degree of bone matrix mineralization and osteocyte lacunar parameters. As individuals age, osteocyte lacunar density decreases, 28 while the proportion of mineralized osteocyte lacunae increases. 29 Since elderly men and women, in particular, are prone to fractures, alterations in osteocyte lacunar density due to T2DM may be a factor contributing to the fracture risk.…”

Section: Introductionmentioning

confidence: 99%

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Lower microhardness along with less heterogeneous mineralization in the femoral neck of individuals with type 2 diabetes mellitus indicates higher fracture risk

Cirovic,

Schmidt,

Vujacic

et al. 2024

JBMR Plus

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There is still limited understanding of the microstructural reasons for the higher susceptibility to fractures in individuals with type 2 diabetes mellitus (T2DM). In this study, we examined bone mineralization, osteocyte lacunar parameters, and microhardness of the femoral neck trabeculae in 18 individuals with T2DM who sustained low-energy fracture (T2DMFx: 78 ± 7 years, 15 women and 3 men) and 20 controls (74 ± 7 years, 16 women and 4 men). Femoral necks of the T2DMFx subjects were obtained at a tertiary orthopedic hospital, while those of the controls were collected at autopsy. T2DMFx individuals had lower trabecular microhardness (p = 0.023) and mineralization heterogeneity (p = 0.001), and a tendency to a lower bone area with mineralization above 95th percentile (p = 0.058) than the controls. There were no significant intergroup differences in the numbers of osteocyte lacunae per bone area, mineralized lacunae per bone area, and total lacunae per bone area (each p > 0.05). After dividing the T2DMFx group based on the presence of vascular complications (VD) to T2DMFxVD (VD present) and T2DMFxNVD (VD absent), we observed that microhardness was particularly reduced in the T2DMFxVD group (vs. control group, p = 0.02), while mineralization heterogeneity was significantly reduced in both T2DMFx subgroups (T2DMFxNVD vs. control, p = 0.002; T2DMFxVD vs. control, p = 0.038). The observed changes in mineralization and microhardness may contribute to the increased hip fracture susceptibility in individuals with T2DM.

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Section: Discussioncontrasting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Lower microhardness along with less heterogeneous mineralization in the femoral neck of individuals with type 2 diabetes mellitus indicates higher fracture risk

Cirovic,

Schmidt,

Vujacic

et al. 2024

JBMR Plus

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“…First, there is a need for more bone biopsies in i) each of the relevant groups (MGUS significantly less frequent than MM ones) and ii) with sufficient quality to obtain a larger sample size and validate the findings of this study. Second, it is important that the bone biopsies are obtained from comparable anatomical sites to minimize the natural osteocyte volume and shape variation within healthy bone 8,28,45 .…”

Section: Discussionmentioning

confidence: 99%

“…One Jamshidi bone biopsy sample (2 mm diameter) was obtained from a patient of the University Hospital Würzburg with myelodysplastic syndrome. Three cylindrical transiliac samples (5 mm diameter) were obtained from an earlier study 8 on autopsy samples at the Ludwig Boltzmann-Institute of Osteology Vienna. Osteocyte lacunae characteristics were re-evaluated in the current study.…”

Section: Patient Sample Collection and Clinical Evaluationmentioning

confidence: 99%

3D osteocyte lacunar morphometry of human bone biopsies with high resolution microCT: from monoclonal gammopathy to newly diagnosed multiple myeloma

Moreno-Jiménez,

Heinig,

Heras

et al. 2023

Preprint

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Osteocytes are bone-embedded cells connected via canaliculi and they regulate the bone resorption/formation balance. Osteocyte function is altered in skeletal disorders including cancer. Multiple myeloma (MM) is a hematological malignancy, whereby plasma cells disrupt the bone homeostasis and induce excessive resorption of the mineralized extracellular matrix (ECM), as evidenced by clinical computed tomography (CT). However, morphometric analyses of the mineralized ECM and bone microporosity are not yet performed in patients with MM, nor in the precursor conditions of monoclonal gammopathy of undetermined significance (MGUS) and smoldering MM (SMM). Here, we characterize changes in 3D osteocyte lacunar morphology in bone biopsy samples obtained from patients with MGUS, SMM and newly diagnosed MM, using high resolution microCT. In the small lacunae 20-900 um3 range, we found a trend for lower lacunar density and increasing 50% cutoff of the lacunar volume cumulative distribution for diseased samples. In the larger lacunae 900-3000 um3 range, we found significantly higher lacunar density and microporosity in the MM group compared to the MGUS/SMM group. Regarding the shape distribution, the MGUS/SMM group showed a trend for flatter, more elongated and anisotropic osteocyte lacunae compared to the control group, though the differences were not statistically significant. Altogether, our findings suggest that osteocytes in human MM bone disease undergo changes in their lacunae morphology, which could be an indicator for impaired bone quality. Future studies are needed to show whether these are consistent within comparable anatomical sites and could serve as a diagnostic or prognostic marker.

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“…Even if 3D imaging techniques are able to provide volumetric information and investigate the 3D bone micro-architectural and structural properties with a non-destructive approach, human bone data remain scarce and are generally limited to a very small field of view [30] . On that basis, a complete morphological analysis of osteocyte lacunae would be of primary interest, since related alterations appear to have an effect on bone remodeling process [31] . Preliminary findings obtained specifically via in situ synchrotron imaging suggest that there exists a specific correlation between osteocyte lacunar morphology/distribution changes and physio-pathological processes.…”

Section: Introductionmentioning

confidence: 99%

Osteoporosis and Covid-19: Detected similarities in bone lacunar-level alterations via combined AI and advanced synchrotron testing

Buccino

Zagra²,

Longo

et al. 2023

Materials & Design

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Osteocyte lacunae in transiliac bone biopsy samples across life span

Cited by 5 publications

References 76 publications

Lower microhardness along with less heterogeneous mineralization in the femoral neck of individuals with type 2 diabetes mellitus indicates higher fracture risk

Lower microhardness along with less heterogeneous mineralization in the femoral neck of individuals with type 2 diabetes mellitus indicates higher fracture risk

3D osteocyte lacunar morphometry of human bone biopsies with high resolution microCT: from monoclonal gammopathy to newly diagnosed multiple myeloma

Osteoporosis and Covid-19: Detected similarities in bone lacunar-level alterations via combined AI and advanced synchrotron testing

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