Osteoconductivity and growth factor production by MG63 osteoblastic cells on bioglass‐coated orthopedic implants

Tan, Fei; Naciri, Mariam; Al‐Rubeai, Mohamed

doi:10.1002/bit.22955

Cited by 41 publications

(33 citation statements)

References 42 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30 Protein adsorption is also enhanced on nanostructured silicon, 31 and nano-features increase the formation and adhesive strength of fibroblast cells. 32 Silica-based scaffolds 33 and coated plates 34 have hydrophilic properties. When we examined the interaction of silica-NP or silica-MP with ADSCs, only silica NPs entered the cells and some were clustered in the vesicles, suggesting that silica NPs enter the cells in part through endocytosis.…”

Section: Discussionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

View full text Add to dashboard Cite

Background Silicon dioxide composites have been found to enhance the mechanical properties of scaffolds and to support growth of human adipose tissue-derived stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) exists as differently sized particles when suspended in culture medium, but it is not clear whether particle size influences the beneficial effect of silicon dioxide on hADSCs. In this study, we examined the effect of different sized particles on growth and mitogen-activated protein kinase signaling in hADSCs. Methods Silica gel was prepared by a chemical reaction using hydrochloric acid and sodium silicate, washed, sterilized, and suspended in serum-free culture medium for 48 hours, and then sequentially filtered through a 0.22 μm filter (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were incubated with silica NPs or 3 μm silica microparticles (MPs), examined by transmission electron microscopy, and assayed for cell proliferation, apoptosis, and mitogen-activated protein kinase signaling. Results Eighty-nine percent of the silica NPs were around 50–120 nm in size. When hADSCs were treated with the study particles, silica NPs were observed in endocytosed vacuoles in the cytosol of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the proliferation of hADSCs, but silica MPs had no significant effect in this regard. Instead, silica MPs induced slight apoptosis. Silica NPs increased phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the ERK1/2 phosphorylation and proliferation induced by silica NPs. Conclusion Scaffolds containing silicon dioxide for tissue engineering may enhance cell growth through ERK1/2 activation only when NPs around 50–120 nm in size are included, and single component silica-derived NPs could be useful for bioscaffolds in stem cell therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…Similar sample dimensions have been used in previous work with both CoBlast and APS studies [39][40][41][42][43]. The substrates were polished with 1200 grit silicon carbide paper.…”

Section: Materials and Sample Preparationmentioning

confidence: 99%

Evaluation and comparison of hydroxyapatite coatings deposited using both thermal and non-thermal techniques

Barry

Twomey

Cowley

et al. 2013

Surface and Coatings Technology

View full text Add to dashboard Cite

This paper compares the properties of hydroxyapatite (HA) coatings, obtained using two different deposition technologies on Ti-6Al-4V substrates. The deposition techniques evaluated were: atmospheric plasma spray (APS, thermal treatment) and a novel micro-blasting technique known as CoBlast (non-thermal treatment). The HA coatings were examined with respect to their morphology, crystallinity and adhesion, while the phase concentration of the metallic substrates were also analysed. In vitro cell proliferation and cell morphology studies using MG-63 osteoblastic cells were carried out on the HA coated substrates obtained using the two deposition techniques, with untreated titanium grade 5 (Ti-6Al-4V) substrates utilised as a control. XRD analysis of the CoBlast deposited HA coatings demonstrated that it was comprised of the same crystalline HA as the precursor powder. For the APS HA coatings however, additional calcium phosphate phases were observed, and these were attributed to phase changes caused by the high plasma deposition temperatures. The APS treated samples also exhibited evidence of substrate modification, with substrate conversion to a β-rich surface at the HA/substrate interface was observed in the XRD analysis. CoBlast HA coatings, with an average thickness of approx. 2.5 µm, were found to have higher tensile adhesion values (33.6 and 35.7MPa), when compared with the 5 MPa obtained for the approx. 26.9 µm thick APS coatings using a modified tensile adhesion test. This lower adhesion tensile value is most likely due to the increased residual stress generated in the HA coating during thermal plasma processing. The cell response studies on the four surfaces tested indicate that the HA surfaces exhibited higher levels of cell proliferation than the untreated titanium after 5 days, with the CoBlast surfaces displaying statically significant increases in cell proliferation.3

show abstract

“…These jets coincidently impinge on a point at the substrate surface resulting in the deposition of a HA coating. To date reports on the CoBlast HA deposition process have focused primarily on titanium and its alloys . The aim of this research is to investigate the potential of the CoBlast HA deposition technique for use with a broader range of metal alloys, specifically: cobalt chrome and SS 316.…”

Section: Introductionmentioning

confidence: 99%

Influence of substrate metal alloy type on the properties of hydroxyapatite coatings deposited using a novel ambient temperature deposition technique

Barry

Cowley

McNally

et al. 2013

J Biomedical Materials Res

View full text Add to dashboard Cite

In addition to titanium grade 5, both cobalt chromium and stainless steel 316 were investigated. In this study HA coatings were deposited using both the CoBlast and plasma sprayed techniques, and the resultant HA coating and substrate properties were evaluated and compared. The CoBlast deposited HA coatings were found to present similar surface morphologies, interfacial properties and composition irrespective of the substrate alloy type. Coating thickness however displayed some variation with the substrate alloy, ranging from 2.0 to 3.0 μm.This perhaps is associated with the electro-negativity of the metal alloys. The APS treated samples exhibited evidence of both coating, and significantly, substrate phase alterations for two metal alloys; titanium grade 5 and cobalt chrome. Conversely, the CoBlast processed samples exhibited no phase changes to the substrates after depositions. The APS alterations were attributed to the brief, but high intensity temperatures experienced during processing.

show abstract

Osteoconductivity and growth factor production by MG63 osteoblastic cells on bioglass‐coated orthopedic implants

Cited by 41 publications

References 42 publications

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Evaluation and comparison of hydroxyapatite coatings deposited using both thermal and non-thermal techniques

Influence of substrate metal alloy type on the properties of hydroxyapatite coatings deposited using a novel ambient temperature deposition technique

Contact Info

Product

Resources

About