Osteoclasts and osteoblasts migrate in opposite directions in response to a constant electrical field

Ferrier, Jack; Ross, Stephen M.; Kanehisa, Junya; Aubin, Jane E.

doi:10.1002/jcp.1041290303

Cited by 145 publications

(89 citation statements)

References 27 publications

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“…Consequently, for bone regeneration, these cells communicate with other bone cells, such as osteoblasts and osteoclasts. The influence of electrical stimulation on bone healing has been studied in vitro [60][61][62][63][64][65][66] and in vivo [67][68][69][70][71][72] and it has been demonstrated that the application of these stimulus can enhance and stimulate osteogenic activities. In this way, the osteoblasts are affected by electromechanical signals to apposite bone tissue [73][74], the piezoelectric nature of bone, leading to natural conversion of the mechanical stimuli into electrical ones.…”

Section: Bonementioning

confidence: 99%

Piezoelectric polymers as biomaterials for tissue engineering applications

Ribeiro

Sencadas

Correia

et al. 2015

Colloids and Surfaces B: Biointerfaces

405

338

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Tissue engineering often rely on scaffolds for supporting cell differentiation and growth. Novel paradigms for tissue engineering include the need of active or smart scaffolds in order to properly regenerate specific tissues. In particular, as electrical and electromechanical clues are among the most relevant ones in determining tissue functionality in tissues such as muscle and bone, among others, electroactive materials and, in particular, piezoelectric ones, show strong potential for novel tissue engineering strategies, in particular taking also into account the existence of these phenomena within some specific tissues, indicating their requirement also during tissue regeneration. This referee reports on piezoelectric materials used for tissue engineering applications. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions. This referee reports on piezoelectric materials used for tissue engineering applications.The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This referee provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions.

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Section: Bonementioning

confidence: 99%

Piezoelectric polymers as biomaterials for tissue engineering applications

Ribeiro

Sencadas

Correia

et al. 2015

Colloids and Surfaces B: Biointerfaces

405

338

View full text Add to dashboard Cite

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“…As far as how the ES signal induced the cytoskeletal reorganization to be large enough to cause significant change in cell shape, the authors attributed it to the change of intracellular free calcium levels. In another study, Ferrier et al (Ferrier et al, 1986) cultured rabbit osteoclasts and rat osteoblast-like cells on glass coverslips in field strengths of 0.1 and 1 V/mm and found that the osteoclasts migrated rapidly toward the positive electrode, while the osteoblast-like cells migrated in the opposite direction toward the negative electrode, showing that different types of bone cells respond differently to the same electrical signal. When salt bridges are used, the so-called faradic products, including hydrogen peroxide, hydroxyl and oxygen ions, free radicals, and other intermediates, are excluded from the culture medium.…”

Section: Salt Bridgementioning

confidence: 99%

Electrical Stimulation in Tissue Regeneration

Meng¹,

Rouabhia²,

Zhang³

2011

Applied Biomedical Engineering

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“…Galvanotaxis and galvanotropism (i.e. EF-mediated migration and shape change, respectively) occur in keratinocytes, epithelial cells, bone cells, chondrocytes and fibroblasts (Chao et al, 2000;Ferrier et al, 1986;Sheridan et al, 1996;Soong et al, 1990;Zhao et al, 1996a). The EF strengths commonly used (1-10 V cm -1 ) are physiologically significant for vertebrates, because 1-2 V cm -1 gradients have been measured on either side of the cut surface of wounds owing to ion flux through leaky cell membranes (Soong et al, 1990) or transepithelial potential driven by Na + pumps (Vanable, 1989).…”

Section: Cdc42 and Its Downstream Effectorsmentioning

confidence: 99%

Roles of microtubules, cell polarity and adhesion in electric-field-mediated motility of 3T3 fibroblasts

Finkelstein

Chang

Chao

et al. 2004

Journal of Cell Science

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Direct-current electric fields mediate motility (galvanotaxis) of many cell types. In 3T3 fibroblasts, electric fields increased the proportion, speed and cathodal directionality of motile cells. Analogous to fibroblasts' spontaneous migration, we initially hypothesized that reorientation of microtubule components modulates galvanotaxis. However, cells with intact microtubules did not reorient them in the field and cells without microtubules still migrated, albeit slowly, thus disproving the hypothesis. We next proposed that, in monolayers wounded and placed in an electric field, reorientation of microtubule organizing centers and stable, detyrosinated microtubules towards the wound edge is necessary and/or sufficient for migration. This hypothesis was negated because field exposure mediated migration of unoriented, cathode-facing cells and curtailed migration of oriented, anode-facing cells. This led us to propose that ablating microtubule detyrosination would not affect galvanotaxis. Surprisingly, preventing microtubule detyrosination increased motility speed, suggesting that detyrosination inhibits galvanotaxis. Microtubules might enhance adhesion/de-adhesion remodeling during galvanotaxis; thus, electric fields might more effectively mediate motility of cells poorly or dynamically attached to substrata. Consistent with this hypothesis, incompletely spread cells migrated more rapidly than fully spread cells. Also, overexpression of PAK4, a Cdc42-activated kinase that decreases adhesion, enhanced galvanotaxis speed, whereas its lack decreased speed. Thus, electric fields mediate fibroblast migration via participation of microtubules and adhesive components, but their participation differs from that during spontaneous motility.

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Osteoclasts and osteoblasts migrate in opposite directions in response to a constant electrical field

Cited by 145 publications

References 27 publications

Piezoelectric polymers as biomaterials for tissue engineering applications

Piezoelectric polymers as biomaterials for tissue engineering applications

Electrical Stimulation in Tissue Regeneration

Roles of microtubules, cell polarity and adhesion in electric-field-mediated motility of 3T3 fibroblasts

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