Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement

Chen, Yawen; Wang, Haicheng; Gao, Lian Jun; Liu, Chang; Jiang, Yiyang; Qu, Hong; Li, Cuiying; Jiang, Jie

doi:10.1371/journal.pone.0153937

Cited by 21 publications

(18 citation statements)

References 34 publications

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“…In 24‐well plates, Raw 264.7 were seeded at 1000 cells well −1 , and recombinant murine RANKL (R&D; 12 ng µL −1 ) was added into the conditioned medium for osteoclastogenic induction. Raw 264.7 were maintained for 10 days and stained using a tartrate‐resistant acid phosphatase (TRAP) Kit (Sigma, St. Louis, MO, USA; Wang & Li , Wang et al 2015a, Wang et al 2015b, Chen et al ). Cells stained with TRAP (Trap+) and containing at least three nuclei (Trap + MNCs) were the osteoclast‐like cells.…”

Section: Methodsmentioning

confidence: 99%

The extra domain A of fibronectin facilitates osteoclastogenesis in radicular cysts through vascular endothelial growth factor

et al. 2019

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Aim To analyse the effects of the alternatively spliced fibronectin (FN) gene and its isoforms on osteoclastogenesis in radicular cysts. Methodology Specimens of radicular cysts were collected surgically from 22 patients whose radiolucent periapical areas were measured on digital panoramic radiographs before surgery. The associations between the radiolucent areas and FN isoforms, vascular endothelial growth factor (VEGF) expression or micro‐vessel density, as well as the relationships amongst them, were analysed by immunohistochemical staining using the antibodies IST‐9, BC‐1, P1F11, VEGF and CD34. Fibroblasts isolated from those specimens were used to induce Trap + MNCs, and the effects of induction were assessed by blocking FN containing extra domain A (EDA + FN), COX‐2 or VEGF in vitro. The effects of EDA exon knockout using CRISPR/Cas system were also assessed. Quantitative PCR was used to analyse relative expression of FN isoforms and osteoclastogenic genes. Data were analysed using linear regression, Spearman's rank correlation analysis, chi‐square test and Student's t‐test; P < 0.05 was considered significant. Results Micro‐vessel density and EDA + FN staining were positively associated with the size of radiolucent periapical areas (mm2; P < 0.05), consistent with a positive association between Trap + MNCs and VEGF expression in fibroblasts (P < 0.05). Blocking the interaction between EDA + FN and fibroblasts inhibited Trap + MNC formation. In addition, EDA exon knockout decreased VEGF expression and inhibited Trap + MNC formation to the extent of blocking VEGF by bevacizumab, but osteoclastogenic induction was restored by recombinant VEGF. Using retrospective clinicopathological data, VEGF staining was shown to be positively associated with EDA + FN staining, micro‐vessel density and the size of radiolucent areas (P < 0.05). Conclusion In fibrous capsules of radicular cysts, the alternatively spliced isoform EDA + FN generated by fibroblasts stimulated VEGF expression via an autocrine effect and then facilitated osteoclastogenesis. Both blockage of VEGF and EDA exon knockout could be used to inhibit bone destruction.

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Section: Methodsmentioning

confidence: 99%

The extra domain A of fibronectin facilitates osteoclastogenesis in radicular cysts through vascular endothelial growth factor

et al. 2019

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“…Four-week-old male BALB/c nude mice were purchased from Vital River Experimental Animal Technique Company (Beijing, China) and maintained in a specific pathogen-free condition. All the animals were acclimatized for 1 week before experiments, and then maintained under controlled temperature (22±2°C), with light dark periods of 12 hours and with free access to water and commercial diet [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Gene editing of the extra domain A positive fibronectin in various tumors, amplified the effects of CRISPR/Cas system on the inhibition of tumor progression

Wang

Peng

et al. 2017

Oncotarget

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BackgroundThe low efficiency of clustered, regularly interspaced, palindromic repeats-associated Cas (CRISPR/Cas) system editing genes in vivo limits the application. A components of the extracellular matrix (ECM), the extra domain A positive fibronectin (EDA+FN), may be a target for CRISPR/Cas system for the pro-oncogenic effects. The exclusion of EDA exon would alter the microenvironment and inhibit tumor progression, even the frequency of gene editing is still limited.ResultsThe pro-oncogenic effects were confirmed by the exclusion of EDA exon from the fibronectin gene, as illustrated by the down-regulated proliferation, migration and invasion of CNE-2Z or SW480 cells (P<0.05). Furthermore, although the efficacy of EDA exon knockout through CRISPR/Cas system was shown to be low in vivo, the EDA+FN protein levels decrease obviously, inhibiting the tumor growth rate significantly (P<0.05), which was accompanied by a decrease in Ki-67 expression and microvessel numbers, and increased E-cadherin or decreased Vimentin expression (P<0.05).Methods and materialsHuman nasopharyngeal carcinoma cell line CNE-2Z, and the colorectal carcinoma cell line SW480 were transfected with CRISPR/Cas9 plasmids targeting EDA exon. The effects of the exclusion of EDA on the cell proliferation, motility and epithelial-mesenchymal transition (EMT) were investigated, and the western blot and real-time PCR were performed to analyze the underlying mechanisms. Furthermore, CRISPR/Cas9 plasmids were injected into xenograft tumors to knockout EDA exon in vivo, and tumor growth, cell proliferation, EMT rate, or vascularization were investigated using western blot, PCR and immunohistochemistry.ConclusionCRISPR/Cas system targeting ECM components was shown to be an effective method for the inhibition of tumor progression, as these paracrine or autocrine molecules are necessary for various tumor cells. This may represent a novel strategy for overcoming the drug evasion or resistance, in addition, circumventing the low efficiency of CRISPR/Cas system in vivo.

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“…7 In addition, the regional acceleratory phenomenon (RAP) is activated by the Review Facial asymmetry correction: From conventional orthognathic treatment to surgery-first approach Tahereh Hosseinzadeh Nik 1 • Elahe Gholamrezaei 1 • Mohammad Ali Keshvad 1 * orthognathic surgery as part of the wound healing process and can play an important role in shortening of the treatment course. 8,9 Evidence shows that the serum level of alkaline phosphatase, which is a key enzyme in bone formation, increases as part of the RAP process four weeks after surgery, and its level remains high for up to four months postoperatively. 10 The absence of a tight occlusion at the time of orthognathic surgery is a double-blade sword.…”

Section: Introductionmentioning

confidence: 99%

Facial asymmetry correction: From conventional orthognathic treatment to surgery-first approach

Nik

Gholamrezaei

Keshvad

2019

J Dent Res Dent Clin Dent Prospects

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The surgery-first approach (SFA), which proceeds without presurgical orthodontic treatment, is assumed to shorten the treatment course because the direction of post-surgical orthodontic tooth movement conforms to the normal muscular forces. Moreover, the regional acceleratory phenomenon (RAP), evoked by surgery, helps in tooth alignment and compensation in a faster way. Although SFA has definite advantages, especially in class III individuals, there is a lack of data about its indications in patients with facial asymmetry. In this article, we reviewed recently published articles on the treatment of asymmetric patients using the SFA. Different aspects, including the three-dimensional assessment of stability in different planes, approaches for fabrication of a surgical splint, predictability of the results, skills needed for bimaxillary surgery, indications as the treatment of choice for condylar hyperplasia, and combination with distraction osteogenesis in candidates with severe asymmetries were found to be the main topics discussed for patients presenting with facial asymmetry

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Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement

Cited by 21 publications

References 34 publications

The extra domain A of fibronectin facilitates osteoclastogenesis in radicular cysts through vascular endothelial growth factor

The extra domain A of fibronectin facilitates osteoclastogenesis in radicular cysts through vascular endothelial growth factor

Gene editing of the extra domain A positive fibronectin in various tumors, amplified the effects of CRISPR/Cas system on the inhibition of tumor progression

Facial asymmetry correction: From conventional orthognathic treatment to surgery-first approach

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