Osteoclast Multinucleation: Review of Current Literature

Kodama, Joe; Kaito, Takashi

doi:10.3390/ijms21165685

Cited by 101 publications

(86 citation statements)

References 208 publications

(299 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DCSTAMP and OCSTAMP proteins represent an interesting group of proteins to study in the context of cell-cell fusion, since they have been shown to play a role in osteoclast and foreign body giant cell (FBGC) fusion 13–15 . They belong to the class of DC-STAMP-like domain-containing proteins, and are multi-pass transmembrane proteins with an intracellular C-terminus containing a non-canonical RING finger domain 13,16,17 . DCSTAMP was shown to localize to the plasma membrane and endoplasmic reticulum (ER) membrane in dendritic cells and osteoclasts 16–19 .…”

Section: Main Textmentioning

confidence: 99%

“…They belong to the class of DC-STAMP-like domain-containing proteins, and are multi-pass transmembrane proteins with an intracellular C-terminus containing a non-canonical RING finger domain 13,16,17 . DCSTAMP was shown to localize to the plasma membrane and endoplasmic reticulum (ER) membrane in dendritic cells and osteoclasts 16–19 . These cell types in Dcstamp KO mice show no apparent defect in differentiation into the osteoclast lineage and cytoskeletal structure, yet osteoclasts and FBGCs are unable to fuse to form terminally differentiated multinucleated cells 14 .…”

Section: Main Textmentioning

confidence: 99%

See 1 more Smart Citation

Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish

Noda

Blaha

Fujihara

et al. 2021

Preprint

View full text Add to dashboard Cite

The process of sperm-egg fusion is critical for successful fertilization, yet the underpinning mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show that both mouse and zebrafish DCST1 and DCST2 are necessary in sperm to fertilize the egg, similar to their orthologs SPE-42 and SPE-49 in C. elegans and Sneaky in D. melanogaster. Mouse Dcst1 and Dcst2 single knockout (KO) spermatozoa are able to undergo the acrosome reaction and show normal relocalization of IZUMO1, an essential factor for sperm-egg fusion, to the equatorial segment. While both single KO spermatozoa can bind to the oolemma, they rarely fuse with oocytes, resulting in male sterility. Similar to mice, zebrafish dcst1 KO males are subfertile and dcst2 and dcst1/2 double KO males are sterile. Zebrafish dcst1/2 KO spermatozoa are motile and can approach the egg, but rarely bind to the oolemma. These data demonstrate that DCST1/2 are essential for male fertility in two vertebrate species highlighting their crucial role as conserved factors in fertilization.

show abstract

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish

Noda

Blaha

Fujihara

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…OCLs develop from haematopoietic stem cells through a series of morphological and functional transformations that lead to the establishment of multinucleated polarized cells (Kodama and Kaito, 2020). These are able to migrate toward bone, adhere to it, and resorb the tissue beneath in a controlled manner (Bruzzaniti and Baron, 2006;Teitelbaum, 2007).…”

Section: The Osteoclast Cellmentioning

confidence: 99%

Role of OSCAR Signaling in Osteoclastogenesis and Bone Disease

Nedeva

Vitale

Elson

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Formation of mature bone-resorbing cells through osteoclastogenesis is required for the continuous remodeling and repair of bone tissue. In aging and disease this process may become aberrant, resulting in excessive bone degradation and fragility fractures. Interaction of receptor-activator of nuclear factor-κB (RANK) with its ligand RANKL activates the main signaling pathway for osteoclastogenesis. However, compelling evidence indicates that this pathway may not be sufficient for the production of mature osteoclast cells and that co-stimulatory signals may be required for both the expression of osteoclast-specific genes and the activation of osteoclasts. Osteoclast-associated receptor (OSCAR), a regulator of osteoclast differentiation, provides one such co-stimulatory pathway. This review summarizes our present knowledge of osteoclastogenesis signaling and the role of OSCAR in the normal production of bone-resorbing cells and in bone disease. Understanding the signaling mechanism through this receptor and how it contributes to the production of mature osteoclasts may offer a more specific and targeted approach for pharmacological intervention against pathological bone resorption.

show abstract

“…The impaired formation F-acting ring also con rmed the ndings above. Moreover, we discovered that CIM downregulated expression of speci c genes in osteoclast, including those involved in regulation of downstream genes expression (Nfatc1, c-Fos and Traf6) 31 , bone resorption function (Cathepsin K and Acp5) 32 , calcium homeostasis (Calcr) 33 , and cell fusion of precursors (Dc-stamp) 34 .…”

Section: Discussionmentioning

confidence: 99%

Cimifugin Suppresses NF-κB Signaling to Prevent Osteoclastogenesis and Periprosthetic Osteolysis

Duan

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Aseptic loosening of prosthesis (ALP) is one of the most common long-term complication of knee and hip arthroplasty. Wear paticle-induced osteoclastogenesis and subsequent periprosthetic osteolysis accounts for the morbidity of ALP. Here, we investigate cimifugin (CIM), a natural extract from Cimicifuga Racemosa and Saposhnikovia Divaricata, as a bone protective drug in treatment of ALP.Method: First, we deployed cell viability and osteoclast formation assays to detect the effect of noncytotoxic CIM on osteoclast differentiation in vitro. Bone slice resorption evaluation and F-actin ring immunofluorescence assays were adopted to measure bone-resorbing function affected by CIM. Then, we introduce quantitative real-time polymerase chain reaction (qRT-PCR) analysis to further identify the repressed osteoclastogenesis by CIM in gene expression level. To reveal underlying mechanism of findings above, we used Western blotting and luciferase reporter gene assay to determine the regulation manner of CIM in NF-κB and MAPKs signaling pathways. Moreover, Ti particle-induced murine calvarial osteolysis model and following histomorphometric analysis via micro-CT and immunohistochemical staining were used to demonstrate the effect of CIM on periprosthetic osteolysis in vivo. Result: CIM administration dose-dependently inhibited both bone marrow-derived macrophages (BMMs) and RAW264.7 cells derived osteoclastogenesis and bone resorption pit formation in vitro, which further supported by reduced expression of F-actin and osteoclast specific genes. According to the Western blotting analysis, inhibition of IkBa phosphorylation in NF-κB signaling pathway, not the phosphorylation of MAPKs, was detected as the suppressive effect of CIM on osteoclastogenesis. In vivo animal experiment demonstrated that CIM alleviated Ti particle-induced bone erosion and osteoclast accumulation in murine calvaria. Conclusion: The current study for the first time proved that CIM could dose-dependently inhibit RANKL-induced osetoclastogenesis via suppressing NF-κB signaling pathway in vitro and prevent periprosthetic osteolysis in vivo. These findings suggest the potential therapeutic use of CIM in ALP.

show abstract

Osteoclast Multinucleation: Review of Current Literature

Cited by 101 publications

References 208 publications

Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish

Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish

Role of OSCAR Signaling in Osteoclastogenesis and Bone Disease

Cimifugin Suppresses NF-κB Signaling to Prevent Osteoclastogenesis and Periprosthetic Osteolysis

Contact Info

Product

Resources

About