Osteochondral repair using an acellular dermal matrix—pilot in vivo study in a rabbit osteochondral defect model

Ye, Ken; Traianedes, Kathy; Robins, Shalley; Choong, Peter F. M.; Myers, Damian E.

doi:10.1002/jor.23837

Cited by 18 publications

(11 citation statements)

References 52 publications

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“…Thirty studies of the retried papers involved rabbits as an animal model [13–42]. The New Zealand white rabbit was adopted in 28/30 studies, whereas Japanese rabbit was used in two related studies [38, 39].…”

Section: Resultsmentioning

confidence: 99%

“…Medial and/or lateral femoral condyles (14/30) and the trochlear groove (16/30) have been the selected anatomical sites where osteochondral defects were created. Between the defects created in the femoral condyles, 3 were made in the load bearing areas [13, 24, 25]. The defects dimension varied from 1.5 mm to 6 mm in diameter and from 1.5 to 10 mm in depth contributing to make the comparison difficult.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, for undecalcified samples, the Stevenel Blue/Van Gieson Pichrofucsin dye [29], thionine staining [27], and Toluidine Blue were used to evaluate the osteochondral compartment. Where specified, all the abovementioned histological stainings were performed after cutting the samples according to a sagittal [14–18, 22–25, 27–30, 38] or longitudinal [13, 40, 41] plane.…”

Section: Resultsmentioning

confidence: 99%

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Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Maglio

Brogini

Pagani

et al. 2019

BioMed Research International

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Osteochondral lesions (OCs) are typically of traumatic origins but are also caused by degenerative conditions, in primis osteoarthritis (OA). On the other side, OC lesions themselves, getting worse over time, can lead to OA, indicating that chondral and OC defects represent a risk factor for the onset of the pathology. Many animal models have been set up for years for the study of OC regeneration, being successfully employed to test different treatment strategies, from biomaterials and cells to physical and biological adjuvant therapies. These studies rely on a plethora of post-explant investigations ranging from histological and histomorphometric analyses to biomechanical ones. The present review aims to analyze the methods employed for the evaluation of OC treatments in each animal model by screening literature data within the last 10 years. According to the selected research criteria performed in two databases, 60 works were included. Data revealed that lapine (50% of studies) and ovine (23% of studies) models are predominant, and knee joints are the most used anatomical locations for creating OC defects. Analyses are mostly conducted on paraffin-embedded samples in order to perform histological/histomorphometric analyses by applying semiquantitative scoring systems and on fresh samples in order to perform biomechanical investigations by indentation tests on articular cartilage. Instead, a great heterogeneity is pointed out in terms of OC defect dimensions and animal's age. The choice of experimental times is generally adequate for the animal models adopted, although few studies adopt very long experimental times. Improvements in data reporting and in standardization of protocols would be desirable for a better comparison of results and for ethical reasons related to appropriate and successful animal experimentation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Maglio

Brogini

Pagani

et al. 2019

BioMed Research International

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“…29 Despite the donor age and disease status, IPFP cells expressed mesenchymal progenitor and pericyte markers, and cell pellets demonstrated chondrogenic and regenerative potential after implantation. IPFP-derived cells have potential for cartilage regeneration when cultured in 3 dimensions in vitro, 17,[36][37][38] in animal models with focal defects, 39,40 and when injected into OA knees of rabbits 41 and humans 42,43 to reduce pain, improve knee function, and reduce the severity of OA progression.…”

Section: Discussionmentioning

confidence: 99%

Development of an Ex Vivo Murine Osteochondral Repair Model

Schaik¹,

Gaul

Dorthé

et al. 2018

CARTILAGE

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Objective Mouse models are commonly used in research applications due to the relatively low cost, highly characterized strains, as well as the availability of many genetically modified phenotypes. In this study, we characterized an ex vivo murine osteochondral repair model using human infrapatellar fat pad (IPFP) progenitor cells. Design Femurs from euthanized mice were removed and clamped in a custom multidirectional vise to create cylindrical osteochondral defects 0.5 mm in diameter and 0.5 mm deep in both condyles. The IPFP contains progenitors that are a promising cell source for the repair of osteochondral defects. For proof of concept, human IPFP-derived progenitor cells, from osteoarthritic (OA) patients, cultured as pellets, were implanted into the defects and cultured in serum-free medium with TGFβ3 for 3 weeks and then processed for histology and immunostaining. Results The custom multidirectional vise enabled reproducible creation of osteochondral defects in murine femoral condyles. Implantation of IPFP-derived progenitor cells led to development of cartilaginous tissue with Safranin O staining and deposition of collagen type II in the extracellular matrix. Conclusions We showed feasibility in creating ex vivo osteochondral defects and demonstrated the regenerative potential of OA human IPFP-derived progenitors in mouse femurs. The murine model can be used to study the effects of aging and OA on tissue regeneration and to explore molecular mechanisms of cartilage repair using genetically modified mice.

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“…Tissue engineering technology has been one of the top research focuses in the field of articular cartilage repair 23 . Stem cells and tissue scaffold were two subtopics occupying the primary portion of the overall publication numbers in the field of cartilage repair 24,25 . The research topic of stem cells topped all topics, accounting for nearly one-third of all published articles.…”

Section: Discussionmentioning

confidence: 99%

Studies of Articular Cartilage Repair from 2009 to 2018: A Bibliometric Analysis of Articles

Duan

Huang

Dong

et al. 2021

Orthopaedic Surgery

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Osteochondral repair using an acellular dermal matrix—pilot in vivo study in a rabbit osteochondral defect model

Cited by 18 publications

References 52 publications

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Development of an Ex Vivo Murine Osteochondral Repair Model

Studies of Articular Cartilage Repair from 2009 to 2018: A Bibliometric Analysis of Articles

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