Osteochondral Integration of Multiply Incised Pure Cartilage Allograft: Repair Method of Focal Chondral Defects in a Porcine Model

Bárdos, Tamás; Farkas, Boglárka; Mezes, Beata; Váncsodi, József; Kvell, Krisztián; Czömpöly, Tamás; Németh, Pèter; Bellyei, A; Illés, Tamás

doi:10.1177/0363546509350045

Cited by 12 publications

(36 citation statements)

References 43 publications

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“…We saw no degeneration of the graft in 24 wk either, an observation that agrees with previous reports for other experimental animals in the literature [4,6] . Further investigation concerning the histological, mechanical and immunohistochemical properties of grafted cartilage needs to be done to verify the longer effects of OA and BGS transplantation, bearing always in mind that in the clinical situation a transplanted knee with osteochondral problems continues to improve even after 6 mo post-operatively.…”

Section: Presence Of Collagensupporting

confidence: 92%

“…After 24 wk the animals were sacrificed using a lethal dose of phenobarbital Ⅳ injection under general anesthesia, which was achieved using the protocol of the surgery [4,14] . The type and degree of integration of reparative tissue were evaluated.…”

Section: Macroscopic/histological Evaluationmentioning

confidence: 99%

“…In the 1950's, the first attempts were made to restore articular cartilage using skin. Even though since then, much progress has been made [1][2][3][4][5][6] , there is still lacking evidence in how to best manage these lesions. The avascular and denervated environment, the immobility of chondrocytes and the limited ability of mature chondrocytes to proliferate, are considered as the main reasons that obstruct intrinsic articular regeneration [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

“…The current treatments include: (1) palliative (debridement), marrow stimulating techniques (microfractures, drilling, abrasion) and transfer of osteochondral unit (autograft or allograft) for lesions up to 2.5 cm 2 ; and (2) for lesions bigger than 2.5 cm 2 , use of chondrogenic potential cells (autologous chondrocyte implantation) and osteochondral allografts [10] . Increasing interest appears in one-time surgery techniques as they provide shorter recovery time, with the transfer of an entire osteochondral autograft being the most appealing [2][3][4][5][6]9,11] . Autografting represents a reasonable solution for osteochondral defects.…”

Section: Introductionmentioning

confidence: 99%

“…No consensus exists regarding the best approach to achieve optimal results [4][5][6]9,12] . The donor site, could be a major source of pain.…”

Section: Introductionmentioning

confidence: 99%

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Donor’s site evaluation after restoration with autografts or synthetic plugs in rabbits

Intzoglou

Mastrokalos

Korres

et al. 2014

WJO

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AIM:To investigate donor site's area histological and immunohistochemical knee cartilage appearances after resurfacing iatrogenic defects with biosynthetic plugs orautografts. METHODS:Thirty New Zealand White rabbits were used in this study. A full-thickness cylindrical defect of 4.5 mm (diameter) × 7 mm (depth) was created with a hand drill in the femoral groove of every animal. In Group A (n = 10) the defect of the donor site was repaired with a biosynthetic osteochondral plug, in Group B (n = 10) with an osteochondral autograft, while in Group C (control group of 10) rabbits were left untreated. RESULTS:Twenty-four weeks postoperatively, smooth articular cartilage was found macroscopically in some trocleas' surfaces; in all others, an articular surface with discontinuities was observed. Twenty-eight out of 30 animals were found with predominantly viable chondrocytes leaving the remaining two -which were found only in the control group-with partially viable chondrocytes. However, histology revealed many statistical differences between the groups as far as the International Cartilage Repair Society (ICRS) categories are concerned. Immunofluoresence also revealed the presence of collagen Ⅱ in all specimens of Group B, whereas in Group A collagen Ⅱ was found in less specimens. In Group C collagen Ⅱwas not found. CONCLUSION:The matrix, cell distribution, subchondral bone and cartilage mineralization ICRS categories showed statistically differences between the three groups. Group A was second, while group B received the best scores; the control group got the worst ICRS scores in these categories. So, the donor site area, when repairing osteochondral lesions with autografting systems, is better amended with osteochondral autograft rather than bone graft substitute implant.

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Section: Presence Of Collagensupporting

confidence: 92%

Section: Macroscopic/histological Evaluationmentioning

confidence: 99%