Osteoblasts attachment on amorphous carbon films

Rodil, S.E.; Ramírez, Claudio; Olivares, Raymundo Cea; Arzate, Higinio; Reyes‐Gasga, J.; Magaña, Carlos

doi:10.1016/j.diamond.2005.10.010

Cited by 15 publications

(12 citation statements)

References 30 publications

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“…Owing to their wide range of properties [9], amorphous carbon surfaces have received a special attention particularly focused on their biocompatibility/ haemocompatibility when used as biomedical implant coatings [10][11][12][13][14][15] or on their interactions with molecular additives in the case of hard disk or solid lubricant coatings [16,17]. Amorphous carbon has also been used as anti-stiction coating in MEMS [18].…”

Section: Introductionmentioning

confidence: 99%

Surface energy and hybridization studies of amorphous carbon surfaces

Zebda

Sabbah

Ababou‐Girard

et al. 2008

Applied Surface Science

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Section: Introductionmentioning

confidence: 99%

Surface energy and hybridization studies of amorphous carbon surfaces

Zebda

Sabbah

Ababou‐Girard

et al. 2008

Applied Surface Science

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“…Numerous in-vitro and in-vivo experiments have indicated that DLC can have both excellent biocompatibility and hemocompatibility [2][3][4][5]. In the past, the wettability of DLC coatings has been modified by selective doping using both metal and non-metal dopants [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…However, in this study the mechanical properties of the modified films were not analyzed. In a different study [5], substrates with various substrate roughness were employed to investigate the attachment of human osteoblast cells to amorphous carbon films with different surface textures. This study concluded that cell attachment increased monotonically with surface roughness.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of hydrogen-free diamond-like carbon films using open-air dielectric barrier discharge atmospheric plasma treatments

Endrino

Marco

Allen

et al. 2008

Applied Surface Science

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A dielectric barrier discharge (DBD) technique has been employed to produce uniform atmospheric plasmas of He and N 2 gas mixtures in open air in order to functionalize the surface of filtered-arc deposited hydrogen-free diamond-like carbon (DLC) films. XPS measurements were carried out on both untreated and He/N 2 DBD plasma treated DLC surfaces. Chemical states of the C 1s and N 1s peaks were collected and used to characterize the surface bonds. Contact angle measurements were also used to record the short-and long-term variations in wettability of treated and untreated DLC.In addition, cell viability tests were performed to determine the influence of various He/N 2 atmospheric plasma treatments on the attachment of osteoblast MC3T3 cells.Current evidence shows the feasibility of atmospheric plasmas in producing long-lasting variations in the surface bonding and surface energy of hydrogen-free DLC and consequently the potential for this technique in the functionalization of DLC coated devices. IntroductionIn a recent study it was shown that the nanoscale wettability of a hydrogenterminated diamond surface was lower than that of regions that had been oxidized using an atomic force microscope tip [1]. The difference in wettability between modified diamond surfaces suggests the application of control wettability of modified carbon surfaces in the fields of medicine and biotechnology, which could also be extended to diamond-like carbon films.Diamond-like carbon (DLC) films are known to be hard, low friction and chemically inert materials. Numerous in-vitro and in-vivo experiments have indicated that DLC can have both excellent biocompatibility and hemocompatibility [2][3][4][5]. In the past, the wettability of DLC coatings has been modified by selective doping using both metal and non-metal dopants [6][7][8]. For instance, both silicon and fluorine can be used to increase the surface energy and reduce the contact angle with water. In one study [6], researchers modified the surface energy of DLC by incorporating oxygen and silicon into the bonding network, thus avoiding the use of harmful fluorine-containing hydrocarbon gases. However, the silicon-containing DLC coatings had 20 times lower wear resistance than undoped DLC films. In other recent study [9], phosphorus-doped DLC films were deposited by applying hybrid plasma immersion ion implantation and deposition (PIIID) techniques; the good wettability exhibited by P-doped DLC samples leads to an increase in hemocompatibility. However, in this study the mechanical properties of the modified films were not analyzed. In a different study [5], substrates with various substrate roughness were employed to investigate the attachment of human osteoblast cells to amorphous carbon films with different surface textures. This study concluded that cell attachment increased monotonically with surface roughness. However, smooth surfaces are critical in the vast majority of biomedical applications and topological functionalization of the surface cannot be seen as a wide-rang...

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“…In addition to surface chemistry, the attachment of osteoblasts is also affected by surface topography (21,24–27). Although a variety of studies (21,25,27–30) have revealed few consistent trends in the effects of surface topography on initial osteoblastic attachment, the most commonly observed trend has been that a porous structure is beneficial to cell attachment (15,17, 21,25,28,29). When the Mn-TiO 2 coating is placed into the culture medium, the porous nanostructured surface facilitates the adsorption of proteins from the culture medium, by providing a larger contact area at the sample-medium interface.…”

Section: Discussionmentioning

confidence: 99%

Integrin-mediated osteoblastic adhesion on a porous manganese-incorporated TiO2 coating prepared by plasma electrolytic oxidation

Zhang

Zhu

et al. 2013

Experimental and Therapeutic Medicine

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This study was conducted to evaluate the bioactivity of manganese-incorporated TiO2 (Mn-TiO2) coating prepared on titanium (Ti) plate by plasma electrolytic oxidation (PEO) technique in Ca-, P- and Mn-containing electrolytes. The surface topography, phase and element compositions of the coatings were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectrometry (EDS), respectively. The adhesion of osteoblast-like MG63 cells onto Ti, TiO2 and Mn-TiO2 surfaces was evaluated, and the signal transduction pathway involved was confirmed by the sequential expression of the genes for integrins β1, β3, α1 and α3, focal adhesion kinase (FAK), and the extracellular regulated kinases (ERKs), including ERK1 and ERK2. The results obtained indicated that Mn was successfully incorporated into the porous nanostructured TiO2 coating, and did not alter the surface topography or the phase composition of the coating. The adhesion of the MG63 cells onto the Mn-incorporated TiO2 coating was significantly enhanced compared with that on the Mn-free TiO2 coating and the pure Ti plates. In addition, the enhanced cell adhesion on the Mn-TiO2 coatings may have been mediated by the binding of the integrin subunits, β1 and α1, and the subsequent signal transduction pathway, involving FAK and ERK2. The study indicated that the novel Mn-TiO2 coating has potential for orthopedic implant applications, and that further investigations are required.

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Osteoblasts attachment on amorphous carbon films

Cited by 15 publications

References 30 publications

Surface energy and hybridization studies of amorphous carbon surfaces

Surface energy and hybridization studies of amorphous carbon surfaces

Functionalization of hydrogen-free diamond-like carbon films using open-air dielectric barrier discharge atmospheric plasma treatments

Integrin-mediated osteoblastic adhesion on a porous manganese-incorporated TiO2 coating prepared by plasma electrolytic oxidation

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