Osteoblastos humanos cultivados sobre arcabouços à base de nanotubos de carbono superhidrofílicos e nanohidroxiapatita

Lobo, Anderson Oliveira; Silva, Gislene R.; Marciano, Fernanda Roberta; Pacheco‐Soares, Cristina

doi:10.17563/rbav.v33i1-2.971

Cited by 1 publication

(2 citation statements)

References 8 publications

(13 reference statements)

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“…This behavior can be attributed to a delay in the repair period of bone lesions. 26 The higher bone density ( Figure 2D), ALP ( Figure 5A), and cortical thickness ( Figure 4A) values suggest a direct relationship between the efficiency of mineralization and the concentration of GNR for the 21-day period, 28,37 which is also consistent with the concentration data presented by the ATR-FTIR and Raman spectra ( Figure 1F and G).…”

Section: In Vivo Studysupporting

confidence: 81%

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<p>High loads of nano-hydroxyapatite/graphene nanoribbon composites guided bone regeneration using an osteoporotic animal model</p>

Oliveira¹,

Carvalho²,

Gusmão

et al. 2019

IJN

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BackgroundIt has been difficult to find bioactive compounds that can optimize bone repair therapy and adequate osseointegration for people with osteoporosis. The nano-hydroxyapatite (nHAp)/carbon nanotubes with graphene oxides, termed graphene nanoribbons (GNR) composites have emerged as promising materials/scaffolds for bone regeneration due to their bioactivity and osseointegration properties. Herein, we evaluated the action of nHAp/GNR composites (nHAp/GNR) to promote bone regeneration using an osteoporotic model.Materials and methodsFirst, three different nHAp/GNR (1, 2, and 3 wt% of GNR) were produced and characterized. For in vivo analyses, 36 Wistar rats (var. albinus, weighing 250–300 g, Comissão de Ética no Uso de Animais [CEUA] n.002/17) were used. Prior to implantation, osteoporosis was induced by oophorectomy in female rats. After 45 days, a tibial fracture was inflicted using a 3.0-mm Quest trephine drill. Then, the animals were separated into six sample groups at two different time periods of 21 and 45 days. The lesions were filled with 3 mg of one of the above samples using a curette. After 21 or 45 days of implantation, the animals were euthanized for analysis. Histological, biochemical, and radiographic analyses (DIGORA method) were performed. The data were evaluated through ANOVA, Tukey test, and Kolmogorov-Smirnov test with statistical significance at P<0.05.ResultsBoth nHAp and GNR exhibited osteoconductive activity. However, the nHAp/GNR exhibited regenerative activity proportional to their concentration, following the order of 3% >2% >1% wt.ConclusionTherefore, it can be inferred that all analyzed nanoparticles promoted bone regeneration in osteoporotic rats independent of analyzed time.

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Section: In Vivo Studysupporting

confidence: 81%

“…Also, the band observed at 635 cm -1 is attributed to deformation vibration of the OHgroups from HAp. 7,28,29 International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 44.224.250.200 on 03-Oct-2020…”

Section: Resultsmentioning

confidence: 99%