Osteoblastic and osteoclastic differentiation of human mesenchymal stem cells and monocytes in a miniaturized three-dimensional culture with mineral granules

Gamblin, Anne Laure; Renaud, Antoine; Charrier, Céline; Hulin, Philippe; Louarn, G.; Heymann, Dominique; Trichet, Valérie; Layrolle, Pierre

doi:10.1016/j.actbio.2014.08.033

Cited by 16 publications

(16 citation statements)

References 41 publications

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“…In contrast to other studies, in which MSC growth inside scaffolds was tested using confocal/electron microscopy or via measurement of total cellular DNA, the use of flow‐cytometry in our study has enabled us to quantitatively assess scaffold colonization by MSCs as well as by other BM cells. In one previous study, a flow‐cytometry assay was used to assess the viability of culture‐expanded MSCs seeded on 3D construct made of a composite of hydroxyapatite and β‐TCP, but the assay was not quantitative …”

Section: Discussionsupporting

confidence: 82%

“…In one previous study, a flowcytometry assay was used to assess the viability of culture-expanded MSCs seeded on 3D construct made of a composite of hydroxyapatite and b-TCP, but the assay was not quantitative. 32 Our data shows that the number of BM MSCs released from Orthoss 1 collagen and Vitoss 1 after 2-week in vitro culture was higher than Orthoss 1 , clearly indicating that collagen-containing scaffolds provided a more favourable environment for MSC attachment and proliferation. Interestingly, the attachment and proliferation of MSCs from BM aspirates into Orthoss 1 collagen and Vitoss 1 were similar despite the difference in their mineral composition.…”

Section: Discussionmentioning

confidence: 57%

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Collagen‐containing scaffolds enhance attachment and proliferation of non‐cultured bone marrow multipotential stromal cells

El‐Jawhari

Sanjurjo‐Rodríguez

Jones

et al. 2015

Journal Orthopaedic Research

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Large bone defects are ideally treated with autografts, which have many limitations. Therefore, osteoconductive scaffolds loaded with autologous bone marrow (BM) aspirate are increasingly used as alternatives. The purpose of this study was to compare the growth of multipotential stromal cells (MSCs) from unprocessed BM on a collagen‐containing bovine bone scaffold (Orthoss® Collagen) with a non‐collagen‐containing bovine bone scaffold, Orthoss®. Another collagen‐containing synthetic scaffold, Vitoss® was included in the comparison. Colonization of scaffolds by BM MSCs (n = 23 donors) was evaluated using microscopy, colony forming unit‐fibroblast assay and flow‐cytometry. The number of BM MSCs initially attached to Orthoss® Collagen and Vitoss® was similar but greater than Orthoss® (p = 0.001 and p = 0.041, respectively). Furthermore, the number of MSCs released from Orthoss® Collagen and Vitoss® after 2‐week culture was also higher compared to Orthoss® (p = 0.010 and p = 0.023, respectively). Interestingly, collagen‐containing scaffolds accommodated larger numbers of lymphocytic and myelomonocytic cells. Additionally, the proliferation of culture‐expanded MSCs on Orthoss® collagen and Vitoss® was greater compared to Orthoss® (p = 0.047 and p = 0.004, respectively). Collectively, collagen‐containing scaffolds were superior in supporting the attachment and proliferation of MSCs when they were loaded with unprocessed BM aspirates. This highlights the benefit of collagen incorporation into bone scaffolds for use with autologous bone marrow aspirates as autograft substitutes. © 2015 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 34:597–606, 2016.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 57%

Collagen‐containing scaffolds enhance attachment and proliferation of non‐cultured bone marrow multipotential stromal cells

El‐Jawhari

Sanjurjo‐Rodríguez

Jones

et al. 2015

Journal Orthopaedic Research

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“…Furthermore, it is widely held that the earlier these defects occur in the osteoblastic lineage, the more undifferentiated or aggressive the cancer cells [15,19-2 0] . Accordingly, more invasive OS cells are noted to have minimal expression of osteocalcin (OCN) and osteopontin (OPN), both of which are observed at higher levels in mature osteoblasts [21][22][23] . Another notable difference between late osteoprogenitors and OS tumor cells is the ability of the latter to evade senescence through an alternative lengthening of telomere (ALT) pathway [24] .…”

Section: Molecular Basis Of Os and Potential Targets For Therapymentioning

confidence: 99%

Molecular pathogenesis and therapeutic strategies of human osteosarcoma

et al. 2016

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Osteosarcoma (OS) is a devastating illness with rapid rates of dissemination and a poor overall prognosis, despite aggressive standard-of-care surgical techniques and combination chemotherapy regimens. Identifying the molecular mechanisms involved in disease pathogenesis and progression may offer insight into new therapeutic targets. Defects in mesenchymal stem cell differentiation, abnormal expression of oncogenes and tumor suppressors, and dysregulation within various important signaling pathways have all been implicated in development of various disease phenotypes. As such, a variety of basic science and translational studies have shown promise in identifying novel markers and modulators of these disease-specific aberrancies. Born out of these and similar investigations, a variety of emerging therapies are now undergoing various phases of OS clinical testing. They broadly include angiogenesis inhibitors, drugs that act on the bone microenvironment, receptor tyrosine kinase inhibitors, immune system modulators, and other radio- or chemo-sensitizing agents. As new forms of drug delivery are being developed simultaneously, the possibility of targeting tumors locallywhile minimizing systemic toxicityis is seemingly more achievable now than ever. In this review, we not only summarize our current understanding of OS disease processes, but also shed light on the multitude of potential therapeutic strategies the scientific community can use to make long-term improvements in patient prognosis.

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“…Physiologically relevant, three-dimensional in vitro models have served as biological and analytical platforms for testing novel treatments and drug delivery systems (10,11). Cell-microsphere constructs formed from human adipose-derived stem cells and gelatin microspheres were recently reported to promote stemness, differentiation and controlled pro-angiogenic potential, and this three-dimensional construct demonstrated enhanced therapeutic potential (12).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva-derived stem cells and osteoprecursor cells

Lee

Park

2017

Experimental and Therapeutic Medicine

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The present study was performed to create stem cell spheroids from human gingiva-derived stem cells and osteoprecursor cells and to evaluate the maintenance of the stemness, the viability and osteogenic differentiation of the cell spheroids. Gingiva-derived stem cells were isolated, and a total of 6×105 stem cells and osteoprecursor cells were seeded into concave micromolds at various ratios. Gingiva-derived stem cells and/or osteoprecursor cells formed spheroids in concave microwells. The spheroids demonstrated a smaller diameter when the number of osteoprecursor cells seeded was lower. The majority of cells in the spheroids were identified to be live cells and the cell spheroids preserved viability throughout the experimental period. The cell spheroids, which contained stem cells, were positive for stem-cell markers. Cell spheroids in concave microwells demonstrated a statistically significant increase in alkaline phosphatase activity as time progressed (P<0.05). A statistically significant difference in phosphatase activity was observed in the stem cell alone group when compared with the osteoprecursor cell group at day 5 (P<0.05). Mineralized extracellular deposits were observed in each group after Alizarin Red S staining. Within the limits of the present study, cell spheroids from gingival cells and osteoprecursor cells maintained shape, viability, stemness and osteogenic differentiation potential.

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Osteoblastic and osteoclastic differentiation of human mesenchymal stem cells and monocytes in a miniaturized three-dimensional culture with mineral granules

Cited by 16 publications

References 41 publications

Collagen‐containing scaffolds enhance attachment and proliferation of non‐cultured bone marrow multipotential stromal cells

Collagen‐containing scaffolds enhance attachment and proliferation of non‐cultured bone marrow multipotential stromal cells

Molecular pathogenesis and therapeutic strategies of human osteosarcoma

Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva-derived stem cells and osteoprecursor cells

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