Osteoblast response to Vitamin D3 loaded cellulose enriched hydroxyapatite Mesoporous silica nanoparticles composite

Sumathra, Murugan; Munusamy, Murugan A.; Alarfaj, Abdulla A.; Rajan, Mariappan

doi:10.1016/j.biopha.2018.04.078

Cited by 31 publications

(20 citation statements)

References 45 publications

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“…It is well known that CDDP fulfills a major purpose in bone mineral homeostasis and that it might in addition perform as a bioactive protein that helps in augmentation of osteogenisis. 39 , 40 …”

Section: Results and Discussionmentioning

confidence: 99%

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration

et al. 2018

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Presently, tissue engineering approaches have been focused toward finding new potential scaffolds with osteoconductivity on bone-disease-affected cells. This work focused on the cisplatin (CDDP)-loaded graphene oxide (GO)/hydroxyapatite (HAP)/chitosan (CS) composite for enhancing the growth of osteoblast cells and prevent the development of osteosarcoma cells. The prepared composites were characterized for the confirmation of composite formation using Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray diffraction techniques. A flowerlike morphology was observed for the GO/HAP/CS-3/CDDP composite. UV–vis spectroscopy was used to observe the controlled release of CDDP from the GO/HAP/CS-3/CDDP composite, and 67.34% of CDDP was released from the composite over a time period of 10 days. The GO/HAP/CS-3/CDDP nanocomposites showed higher viability in comparison with GO/HAP/CS-3 on MG63 osteoblast-like cells and higher cytotoxicity against cancer cells (A549). The synthesized composite was found to show enhanced proliferative, adhesive, and osteoinductive effects on the alkaline phosphatase activity of osteoblast-like cells. Our results suggested that the CDDP-loaded GO/HAP/CS-3 nanocomposite has an immense prospective as a bone tissue replacement in the bone-cancer-affected tissues.

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Section: Results and Discussionmentioning

confidence: 99%

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration

et al. 2018

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“…Specifically, it was found that VD3 in combination with nano-HAP loaded on polycaprolactone/gelatin scaffolds stimulated osteogenesis in adipose tissue MSCs, as evidenced by increased ALP activity and mineralization, as well as gene expression of osteogenic markers (e.g., COLL I, ALP, RUNX2, BGLAP) [ 6 ]. Similarly, VD3 loaded on cellulose-enriched HAP/silica scaffolds induced increased osteogenesis of an osteoblast-like cell line (MG63), as evidenced by elevated expression of the ALP, RUNX2, and OCN genes [ 21 ], while VD3/vitamin K2/Mg-loaded polymers’ composite nanofibers supported osteogenic differentiation of BM-MSCs, increasing the expression of RUNX2, BMP2, and osteopontin [ 20 ]. In addition, human dental pulp MSCs grown on chitosan scaffolds constructed to sustainably deliver VD3 over the course of 3–5 days showed higher expression of ALP, RUNX2, and OCN, even when cultured in standard growth medium [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, polycaprolactone/gelatin scaffolds loaded with VD3 in combination with nano-hydroxyapatite (HAP) stimulated osteogenesis of adipose tissue MSCs, increasing the ALP activity in the early stages and mineralization in the late stages of differentiation [ 6 ]. Similarly, an osteoinductive effect was demonstrated for human BM-MSCs grown on VD3/vitamin K2/Mg-loaded biodegradable polymer nanofibers [ 20 ], as well as on human dental pulp MSCs seeded on chitosan scaffolds constructed to sustainably deliver VD3 over the course of 3–5 days [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling

et al. 2022

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The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.

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“…Sumathra et al reported novel scaffolds for the loading and delivery of vitamin D3 using cellulose/hydroxyapatite/mesoporous silica nanoparticles (C/HAp/mesoporous SNPs). The proliferation and adhesion of bone stem cells (MG63), and the osteoinductive activation of alkaline phosphatase were stimulated, leading them to propose that the vitamin D3-loaded scaffolds could be used for treatment of bone defects [91].…”

Section: Delivery Of Small Molecules and Nucleic Acidsmentioning

confidence: 99%

Recent advances in the application of mesoporous silica-based nanomaterials for bone tissue engineering

Eivazzadeh‐Keihan

Chenab

Mosafer

et al. 2020

Materials Science and Engineering: C

144

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Silica nanomaterials (SNMs) and their composites have recently been investigated as scaffolds for bone tissue engineering. SNM scaffolds possess the ability to encourage bone cell growth and also allow the simultaneous delivery of biologically active biomolecules that are encapsulated in the mesopores. Their high mechanical strength, low cytotoxicity, ability to stimulate both the proliferation and osteogenic differentiation of progenitor cells make the SNMs appropriate scaffolds. Their physiochemical properties facilitate the cell spreading process, allow easy access to nutrients and help the cell-cell communication process during bone tissue engineering. The ability to deliver small biomolecules, such as dexamethasone, different growth factors, vitamins and mineral ions depends on the morphology, porosity, and crystallinity of SNMs and their composites with other polymeric materials. In this review, the abilities of SNMs to perform as suitable scaffolds for bone tissue engineering are comprehensively discussed.

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Osteoblast response to Vitamin D3 loaded cellulose enriched hydroxyapatite Mesoporous silica nanoparticles composite

Cited by 31 publications

References 45 publications

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration

Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling

Recent advances in the application of mesoporous silica-based nanomaterials for bone tissue engineering

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