Osteoblast-like cell ingrowth, adhesion and proliferation on porous Ti-6Al-4V with particulate and fiber scaffolds

Chen, Jianbo; Paetzell, Emily; Zhou, Jianxin; Lyons, Lauren; Soboyejo, W. O.

doi:10.1016/j.msec.2010.01.005

Cited by 24 publications

(23 citation statements)

References 36 publications

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“…The adhesion, proliferation and differentiation of human bone marrow stromal cells (hBMSCs) on BP‐BG scaffolds were visualized by CLSM ( Figure a). A dense layer of hBMSCs spread and grow on the surface of BP‐BG scaffolds uniformly on day 0, featuring phenotype characteristics of the healthy striation of the cytoskeleton, which demonstrates that an interconnected and hierarchical porous structure of BP‐BG scaffold is favorable for cell infiltration and proliferation in a 3D‐penetrating microenvironment, indicating the desirable osteoconduction performance of BP‐BG scaffolds. Moreover, the cytoskeleton of cells shrinks and turns to be spherical during 5 d incubation in osteogenic induction medium, accompanied by the disappearance of tentacle, while hBMSCs growing on the BG scaffolds display negligible change of phenotype on day 5 (Figure S17, Supporting Information), demonstrating the vital role of BP nanosheets played in accelerating the osteogenic differentiation, and the excellent osteoinduction performance of BP‐BG scaffolds.…”

Section: Methodsmentioning

confidence: 95%

2D‐Black‐Phosphorus‐Reinforced 3D‐Printed Scaffolds:A Stepwise Countermeasure for Osteosarcoma

et al. 2018

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With the ever-deeper understanding of nano-bio interactions and the development of fabrication methodologies of nanomaterials, various therapeutic platforms based on nanomaterials have been developed for next-generation oncological applications, such as osteosarcoma therapy. In this work, a black phosphorus (BP) reinforced 3D-printed scaffold is designed and prepared to provide a feasible countermeasure for the efficient localized treatment of osteosarcoma. The in situ phosphorus-driven, calcium-extracted biomineralization of the intra-scaffold BP nanosheets enables both photothermal ablation of osteosarcoma and the subsequent material-guided bone regeneration in physiological microenvironment, and in the meantime endows the scaffolds with unique physicochemical properties favoring the whole stepwise therapeutic process. Additionally, a corrugated structure analogous to Haversian canals is found on newborn cranial bone tissue of Sprague-Dawley rats, which may provide much inspiration for the future research of bone-tissue engineering.

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Section: Methodsmentioning

confidence: 95%

2D‐Black‐Phosphorus‐Reinforced 3D‐Printed Scaffolds:A Stepwise Countermeasure for Osteosarcoma

et al. 2018

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“…Chen et al reported the steady growth of human osteosarcoma (HOS) cells up to 9 days onto particulate porous Ti6Al4V with pore diameter of 100, 130, and 150 µm and the porosity of 46, 44, and 41%, respectively. 25) They also reported slightly better cell growth on the porous Ti6Al4V with 150 µm pores followed by that of 130 µm after 5 and 9 days of incubation. 25) Ryan et al reported the steady growth of SaOS-2 cells on cylindrical porous Ti with mean pore size of 465 µm and the porosity of 45.1%.…”

Section: Compression Test Conditionmentioning

confidence: 92%

“…25) They also reported slightly better cell growth on the porous Ti6Al4V with 150 µm pores followed by that of 130 µm after 5 and 9 days of incubation. 25) Ryan et al reported the steady growth of SaOS-2 cells on cylindrical porous Ti with mean pore size of 465 µm and the porosity of 45.1%.…”

Section: Compression Test Conditionmentioning

confidence: 92%

Cell Proliferation, Corrosion Resistance and Mechanical Properties of Novel Titanium Foam with Sheet Shape

et al. 2012

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We had developed novel titanium (Ti) foam sheet with original slurry foaming method. The products had an average pore size under 500 µm in diameter, volumetric porosities over 80%, and chemical composition corresponding to grade 4 in ISO 5832-2; Implants for surgery ® Metallic materials. The Ti foam had the tensile strength of 9 to 20 MPa similar to cancellous bone strength and anisotropic characteristics. Considering the application of our Ti foam for surface modification of hip or knee prostheses, we prepared Ti foam sheet joined with CP Ti, Ti 6Al4V, or Zr2.5Nb plate to evaluate the effect of joined solid metals on cell proliferation and corrosion resistance. Plate materials did not show any significant difference on cell proliferation test but affected the anode corrosion rates. Our novel Ti foam exhibited high applicability to the surface modification not only of Ti but also of Ti6Al4V and Zr2.5Nb orthopedic implants.

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“…That makes Ti-10Mo alloy have higher safety than Ti-15Mo alloy in the practical clinic application. Additionally, it has been demonstrated that the corrosion resistance of Ti-Mo alloys is superior to those of CP-Ti and Ti-6Al-4V due to the spontaneously formed stable TiO 2 -MoO 3 passive film on its surface [42][43][44][45][46]. This spontaneously formed oxide film can provide a bio-inert layer in the aggressive body fluids, effectively inhibiting the release of metal ions.…”

Section: In Vitro Biocompatibility Evaluationmentioning

confidence: 99%

Porous Ti-10Mo alloy fabricated by powder metallurgy for promoting bone regeneration

Liu

et al. 2019

Sci. China Mater.

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Porous Ti-10Mo alloys were fabricated by powder metallurgy using a space-holder method. The pore characteristics, microstructure, mechanical properties, in vitro biocompatibility, and in vivo osseointegration of the fabricated alloys were systematically investigated. The results show that with different weight ratios of the space-holder (NH 4-HCO 3) added, all of the porous Ti-10Mo alloys sintered at 1,300°C exhibited a typical Widmanstätten microstructure. The porosity and average pore size of the porous structures can be controlled in the range of 50.8%-66.9% and 70.1-381.4 μm, respectively. The Ti-10Mo alloy with 63.4% porosity exhibited the most suitable mechanical properties for implant applications with an elastic modulus of 2.9 GPa and a compressive yield strength of 127.5 MPa. In vitro, the alloyconditioned medium showed no deleterious effect on the cell proliferation. The cell viability in this medium was higher than that of the reference group, suggesting non-toxicity and good biological characteristics of the alloy specimens. In vivo, after eight weeks' implantation, new bone tissue formed surrounding the alloy implants, and no noticeable inflammation was observed at the implantation site. The bone bonding strength of the porous Ti-10Mo alloy increased over time from 46.6 N at two weeks to 176.4 N at eight weeks. Suitable mechanical properties together with excellent biocompatibility in vitro and osteointegration in vivo make the porous Ti-10Mo fabricated by powder metallurgy an attractive orthopedic implant alloy.

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Osteoblast-like cell ingrowth, adhesion and proliferation on porous Ti-6Al-4V with particulate and fiber scaffolds

Cited by 24 publications

References 36 publications

2D‐Black‐Phosphorus‐Reinforced 3D‐Printed Scaffolds:A Stepwise Countermeasure for Osteosarcoma

2D‐Black‐Phosphorus‐Reinforced 3D‐Printed Scaffolds:A Stepwise Countermeasure for Osteosarcoma

Cell Proliferation, Corrosion Resistance and Mechanical Properties of Novel Titanium Foam with Sheet Shape

Porous Ti-10Mo alloy fabricated by powder metallurgy for promoting bone regeneration

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