Osteoblast differentiation of bone marrow stromal cells cultured on silica gel and sol–gel-derived titania

Dieudonné, S.C.; Dolder, Juliette van den; Ruijter, J.E. de; Paldan, Hannu; Peltola, Timo; Hof, M.A. van ‘t; Happonen, Risto‐Pekka; Jansen, J.A.

doi:10.1016/s0142-9612(02)00068-6

Cited by 70 publications

(52 citation statements)

References 26 publications

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“…The thickness of the bone-like hydroxyapatite may reach up to 10 mm after 2 weeks in simulated body fluid [114]. Cell culturing tests indicated that the sol-gel titania was compatible with bone cells and was able to facilitate osteogenesis of bone precursor cells [117]. In vivo, the calcium phosphate was observed within the titania gel film after 12 weeks of implantation in femurs of goals by Li et al [118].…”

Section: Tio 2 Coatingmentioning

confidence: 97%

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Liu

Chu

Ding

2004

Materials Science and Engineering: R: Reports

3,023

1,477

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Titanium and titanium alloys are widely used in biomedical devices and components, especially as hard tissue replacements as well as in cardiac and cardiovascular applications, because of their desirable properties, such as relatively low modulus, good fatigue strength, formability, machinability, corrosion resistance, and biocompatibility. However, titanium and its alloys cannot meet all of the clinical requirements. Therefore, in order to improve the biological, chemical, and mechanical properties, surface modification is often performed. This article reviews the various surface modification technologies pertaining to titanium and titanium alloys including mechanical treatment, thermal spraying, sol-gel, chemical and electrochemical treatment, and ion implantation from the perspective of biomedical engineering. Recent work has shown that the wear resistance, corrosion resistance, and biological properties of titanium and titanium alloys can be improved selectively using the appropriate surface treatment techniques while the desirable bulk attributes of the materials are retained. The proper surface treatment expands the use of titanium and titanium alloys in the biomedical fields. Some of the recent applications are also discussed in this paper. #

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Section: Tio 2 Coatingmentioning

confidence: 97%

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Liu

Chu

Ding

2004

Materials Science and Engineering: R: Reports

3,023

1,477

View full text Add to dashboard Cite

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“…At the timepoints indicated in the figures, RBM cells were lysed with CelLytic TM -M (Sigma Chemical Corp.) according to the manufacturer's specifications, and aliquots of the supernatant were used for ALP and cellular protein measurements as described previously (3,20). ALP activity was determined spectrophotometrically at 405 nm by measuring the conversion to p-nitrophenol from p-nitrophenylphosphate (pNPP; Sigma Chemical Corp.) after combining 20 μL of supernatant with 180 μL buffersubstrate solution at 37°C (pH 10.4) in 96-well plates using a microplate reader.…”

Section: Quantification Of Alkaline Phosphatase (Alp) Activitymentioning

confidence: 99%

The function of connexin 43 on the differentiation of rat bone marrow cells in culture

Kamijo¹,

Haraguchi²,

Tonogi³

et al. 2006

Biomed. Res.

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Connexin (Cx) 43-mediated gap-junctional intercellular communication (GJC) mainly regulates the osteoblastic differentiation, but much of the function of Cx43 on the differentiation of bone marrow cells is unclear. This study is aimed to clarify relationship between the differentiation of rat bone marrow cells and the function of Cx43. Bone marrow cells derived from four-week-old Wistar strain rats were grown in the presence and absence of 18-α-glycyrrhetinic acid (AGA, 100 μM) to inhibit Cx43-mediated GJC. Expression of Cx43 gene and protein, and the level of intracellular cyclic adenosine monophosphate (cAMP) were determined as the assessment of the function in Cx43-mediated GJC, and alkaline phosphatase (ALP) activity and mineralization were measured as the assessment of osteoblastic differentiation. The Cx43 gene expression was first observed at 2 days, but under the condition in which rat bone marrow cells were treated with AGA, there was no significant effect on the Cx43 gene expression. By administrating AGA to rat bone marrow cells, all parameters of maturation but the Cx43 gene expression significantly decreased. The results of this experiment suggest that Cx43-mediated GJC plays a critical role in rat bone marrow cells, progress toward maturation.

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“…4 The sol-gel method has also been used to produce bioactive porous glasses for bone regeneration and to fabricate silica films on glass surfaces for cell-growth. 5,6 It has been observed that biological entities such as proteins and liposomes, 7,8 in addition to mammalian and prokaryotic cells, remain active on sol-gel derived surfaces after immobilisation or adhesion and can demonstrate increased stability and growth.…”

Section: Introductionmentioning

confidence: 99%

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

et al. 2012

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We demonstrate a straightforward procedure for the controlled formation of silica films on tissue culture polystyrene (PS) surfaces. The films were formed by sequentially treating PS with polyaniline, glutaric dialdehyde and protein prior to silica formation. The films could be tailored to exhibit superhydrophilicity (contact angle < 5 ) which was retained for more than two months under ambient conditions. Both hydrophilic and super-hydrophilic silica coated surfaces were suitable for the culture of an adherent human melanoma cell line. Proliferation, toxicity and adhesion assays were used to compare cell behaviour. Cells on the silica surfaces showed enhanced adhesion and comparable rates of cell proliferation as compared to cells grown on conventional tissue culture plastic. The results obtained can be understood by considering the surface properties of the different materials and the ability of the silica coated surfaces to adsorb significantly higher levels of serum proteins from the growth medium.One of the outcomes of this study is a re-evaluation of the hydrophobicity/hydrophilicity characteristics required for good cell growth and the possibility of designing new tissue culture materials capable of greater control over cell populations.

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Osteoblast differentiation of bone marrow stromal cells cultured on silica gel and sol–gel-derived titania

Cited by 70 publications

References 26 publications

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

The function of connexin 43 on the differentiation of rat bone marrow cells in culture

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

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