Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb:YAG laser irradiation

Mariscal-Muñoz, Eduardo; Costa, Ciniro; Tavares, Hewerson Santos; Bianchi, Jonas; Hebling, Josimeri; Machado, João Paulo Barros; Lerner, Ulf H.; Souza, Pedro Paulo Chaves de

doi:10.1007/s00784-015-1533-1

Cited by 37 publications

(33 citation statements)

References 39 publications

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“…In vitro studies show that osteogenic differentiation of osteoblast and mesenchymal stem cells is promoted by Ti surface with moderate micron scale roughness, hydrophilic surfaces, and surfaces containing nanostructures [2][3][4][5] . Angiogenesis or formation of new blood vessels is an important process, which crucially influences the process of bone healing and tissue regeneration around dental implant 6) .…”

Section: Introductionmentioning

confidence: 99%

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

et al. 2017

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Angiogenesis play a crucial role in the regeneration of hard and soft tissue around dental titanium (Ti) implant. Enamel matrix derivative (EMD) promotes tissue regeneration and stimulates angiogenesis but its effect on the angiogenesis on Ti surfaces was never investigated. The effect of EMD on the angiogenic activity of endothelial cells cultured on pre-treated smooth Ti (PT), acidetched (A), coarse-grit blasted and acid-etched (SLA) surfaces and tissue culture plastic (TCP) in the presence or absence of EMD was investigated. EMD inhibited the proliferation/viability of human umbilical vein endothelial cells (HUVECs) growing on A and SLA Ti surfaces. EMD induced an increase in the expression of all these genes in HUVECs grown on SLA surface but not on other surfaces. Summarizing, our data show that EMD influences proliferation and expression of angiogenesis associated gene in HUVECs grown on moderately rough SLA surfaces, suggesting that EMD might promote angiogenesis following implantation.

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Section: Introductionmentioning

confidence: 99%

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

et al. 2017

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“…Previous studies by other investigators have dealt with the production and characterization of laser‐induced micro‐ and nanotextured titanium surfaces, many of them focusing on the treatment of titanium that was already chemically modified, i.e., sandblasted, large grit, acid‐etched Ti, or employing different types of lasers (CO 2 ; Er:yttrium aluminum garnet (YAG); Yb:YAG; Er, Cr:yttrium scandium gallium garnet (YSGG); Nd:YAG) . The patterns obtained in these works ranged between about 400 nm and 60 µm in size.…”

Section: Discussionmentioning

confidence: 99%

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

Jablonski

Wedemeyer²,

Rekasi

et al. 2018

Adv Materials Inter

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The stability of orthopedic implants is governed by the interaction between the material's surface and bone cells. Nanosized structures seem to be promising in supporting the osseointegration of total joint replacements. In order to analyze whether nanostructured titanium surfaces, as generated by a short‐pulsed Nd:YVO4 laser irradiation process developed in the aeronautics and space industry as a “cold ablation” process, can be useful for orthopedic applications, their impact on bone cells is evaluated in vitro. Cell spreading and morphology on nanostructured titanium niobium nitride or titanium plasma‐sprayed surfaces are comparable to osteoblast behavior on corresponding untreated titanium or cells grown on cover glass. However, on the nanostructured surfaces cell numbers appear to be reduced. Nonetheless, cell viability is not affected by laser pretreatment. Interestingly, osteoblast proliferation on nanostructured titanium is inhibited to the benefit of an increased production of osteoblast‐specific proteins such as bone alkaline phosphatase, osteocalcin, or procollagen. Furthermore, an enhanced mineralization of cells grown on nanostructured surfaces is confirmed in terms of an elevated hydroxyapatite deposition. Laser irradiation leads to an additional nanostructure on microstructured titanium surfaces and might positively affect the biocompatibility and ingrowth of prostheses made from the corresponding biomaterials.

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“…In a previous study, Muñoz et al showed that a rough cpTi surface post-laser surface treatment (R a : 10.57 AE 0.39 mm) as compared to smooth (polished) ones (R a : 0.32 AE 0.01 mm) resulted in greater ALP activity as well as higher expression of osteoblastic phenotype markers. 68 Yin et al also observed higher average ALP activity as well as upregulation in osteoblast gene expression of human osteoblast-like cells on rough Ti alloy surfaces aer electrochemically etching or sandblasting and acid-etching as compared to machined (smooth) surfaces. 69 Furthermore, an increase in surface roughness was also shown to yield similar ALP activity levels for both sandblasted and acid-etched cpTi and Y-ZrO 2 surfaces aer 8, 11, and 15 days.…”

Section: Mammalian Cellular Growthmentioning

confidence: 96%

Biological characterization of surface-treated dental implant materials in contact with mammalian host and bacterial cells: titanium versus zirconia

et al. 2019

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Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb:YAG laser irradiation

Abstract: Laser treatment of titanium generates a reproducible and efficient surface triggering osteoblast differentiation that can be of importance for osteointegration.

Cited by 37 publications

References 39 publications

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

Biological characterization of surface-treated dental implant materials in contact with mammalian host and bacterial cells: titanium versus zirconia

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