Osteoblast conditioned media contain TGF-?1 and modulate the migration of prostate tumor cells and their interactions with extracellular matrix components

Cell adhesion and chemotaxis in prostate cancer metastasis to bone: a minireview

“…13 TGF-B and EGF are chemotactic for prostate cancer cells. 16,18 Several cell adhesion molecules have been identi®ed as playing a role in bone metastasis of a variety of tumors. Galectin-3, VCAM-1, CD11a, CD18, integrins and LFA-1 have been implicated in prostate cancer adhesion to bone derived endothelial cells.…”

“…The difference between this study and Rajan et al's could be explained by the use of different types of prostate cancer cell lines. 16,18 Together, these two investigations demonstrate the heterogeneity of prostate cancer cell lines because TGF-b 1 was a chemoattractant for and EGF was a chemoattractant for TSU-pr 1. Another study demonstrated that bone-derived insulin-like growth factor I and II increased chemotaxis of PC-3, LNCaP (derived from prostatic lymph node metastasis), and DU-145 (derived from prostatic brain metastasis).…”

“…Festuccia et al identi®ed transforming growth factor (TGF)-beta-1 as a chemoattractant for bone metastasizing PC-3 cells (Table 1). 18 Osteoblast conditioned media enhanced the chemomigration and invasion of reconstituted basement membrane. The increased chemotaxis and invasion was blocked by neutralizing TGF-b 1 polyclonal antibody but not by elution of osteoblast conditioned media in agarose ± heparin columns.…”

“…19 The chemotactic activity of IGF I and II was not evaluated in studies mentioned earlier. 16,18 Therefore, it is possible that IGF I and II have a chemotactic effect on TSU-pr1 cells as well.…”

“…18 Conditioned media produced by osteoblasts was shown to contain TGF-beta. In addition to promoting PC-3 chemotaxis, it increased PC-3 cell adhesion and spreading on matrigel, laminin, collagen IV and collagen I. Immuno¯uorescence analysis demonstrated that alpha-2 beta-1 and alpha-3 beta-1 integrins were upregulated in TGF-beta treated cells.…”