Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

Abstract

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affi nity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

Profiling the miRNA-mRNA-lncRNA interaction network in MSC osteoblast differentiation induced by (+)-cholesten-3-one

“…Using in vivo and in vitro analyses, Xi et al indicated that the PI3K/Akt cell signalling pathway is involved in osteoporosis inhibition by promoting osteoblast proliferation, differentiation and bone formation [ 47 ]. The calcium signalling pathway and mineral absorption have also been reported previously [ 48 , 49 ].…”