Osteoblast cell response to oxide films formed on nanograin 316L stainless steel obtained by two-dimensional linear plane-strain machining

Abstract

a b s t r a c tThe long-term success of orthopedic implants is strongly determined by their surface characteristics and the interaction of this surface with the surrounding biological environment. The biocompatibility of metallic biomaterials is mainly due to their ability to form an adherent oxide layer on their surface that is in direct contact with the biological environment. In this study, oxide layers were grown on ultra-fine grained 316L stainless steel samples, naturally, chemically and thermally. Samples in three different nanoscale average grain sizes were obtained by severe plastic deformation using linear plane-strain machining technique. Refining the grain size along with growing the oxide layer create surfaces with a wide variety of surface topography, roughness and chemistry. After chemical treatment, a chromiumenriched oxide with island-like topography was formed on the surface and substrates with smaller average grain size demonstrated rougher surfaces. After thermal treatment, micro-scaled oxide grains were formed on the surface and the amount of manganese oxide in its composition was increased. MC3T3 cell adhesion was greatly improved on the native oxide formed on the sample with the smallest average grain size. The oxide layer that is naturally formed on the surface demonstrated higher biocompatibility compared to both thermally and chemically formed oxides.Published by Elsevier B.V.

Assessment of mechanical and biocompatible performance of ultra-large nitinol endovascular devices fabricated via a low-energy laser joining process

“…12 Cell cytotoxicity was assessed by a colorimetric assay using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT, CT02 MTT Cell Growth Assay Kit, EMD Millipore, Darmstadt, Germany). 13 After 48 hours incubation time, 0.01 ml pre-warmed MTT solution was added to each culture well containing 0.1 ml fresh cell medium and the cell culture plate containing MTT solution continued incubation for 4 hrs under the same conditions. MTT solution has a pale yellow color which live cells cleave it to yield a dark blue formazan product.…”

Production and Properties of the Porous Layer Obtained by the Electrochemical Method on the Surface of Austenitic Steel

“…The great advantage of porous layers produced by micro-arc oxidation is the enhanced corrosion resistance [ 50 , 51 , 52 , 53 ], which is an important aspect in the selection of components used for medicine and other industries. Unfortunately, there is little literature on porous layers’ characteristics on stainless steel produced by the micro-arc oxidation.…”

Corrosion Resistance of Anodic Layers Grown on 304L Stainless Steel at Different Anodizing Times and Stirring Speeds

“…On the other hand, in terms of biomedical applications, Asoh et al [29] reported the growth of anodic layers on type 304 stainless steel in an anodizing bath of hydrogen peroxide and sulfuric acid is suitable to prepare the surface prior to the deposit of synthetic bioactive hydroxyapatite in biomedical implant devices. However, even though corrosion resistance is an important issue in medical devices and applications [32,33], literature is scarce in the electrochemical characterization of anodic layers on stainless steel.…”