Osteoblast attachment to a textured surface in the absence of exogenous adhesion proteins

Abstract

The present study investigated whether osteoblasts could attach to a culture substratum through a surface texture-dependent mechanism. Four test groups were used: (A) untextured, and three texture groups with maximum feature sizes of (B) <0.5 microm, (C) 2 microm, and (D) 4 microm, respectively. All surfaces were coated with the nonadhesive protein bovine serum albumin (BSA). Osteoblasts were allowed to adhere in serum-free medium for either 1 or 4 h, at which time nonadherent cells were removed. At 4 h, untextured surface A exhibited no cell attachment, while textured surfaces B, C, and D exhibited 9%, 32%, and 16% cell adhesion, respectively. At 16 h of incubation, adherent osteoblasts on textured surface C exhibited focal adhesion contacts and microfilament stress-fiber bundles. These results indicate that microtextured surfaces in the absence of exogenous adhesive proteins can facilitate osteoblast adhesion.

Enhanced biological responses of a hydroxyapatite/TiO<Sub>2</Sub> hybrid structure when surface electric charge is controlled using radiofrequency sputtering

“…Albumin binds with lipids, regulates cytoplasmic calcium oscillations, and stimulates proliferation of osteoblasts 39) . Albumin also enhances fibronectin-integrin interactions.…”

Titanate nanowire scaffolds decorated with anatase nanocrystals show good protein adsorption and low cell adhesion capacity

“…Stem/ osteoprogenitor cells attach to the implant surface via interaction of cell integrins with adsorbed proteins, the extent of which may consequently affect the spread, proliferation, and differentiation of cells. [23][24][25][26] Osteoblast attachment on titanium surfaces in the presence of preadsorbed proteins was observed to be enhanced compared with osteoblast attachment on control titanium surfaces without preadsorbed proteins. 27 However, ANs@TiNWs with high protein adsorption showed low MG63 cell adhesive capacity in our study, which may be due to the special nanostructure of the ANs@ TiNWs, with their larger specific surface area favoring protein adsorption but limited binding sites available for adhesion of MG63 cells.…”

Hydroxyapatite-Nanotube Composites and Coatings for Orthopedic Applications

“…show normal growth and proliferation on CNTs [116][117][118][119]. Figure 2.25 shows fibroblast cells grown on vertically aligned carbon nanotubes [116].…”

“…Figure 2.25 shows fibroblast cells grown on vertically aligned carbon nanotubes [116]. Recent studies have reported increased proliferation and adhesion of osteoblast cells on CNT surface, due to the selective absorption of proteins on the CNT surface from cell culture medium [120][121].…”

“…Especially, for anchorage-dependent cells, like osteoblasts, chondrocytes etc., initial cell-biomaterial interaction and attachment regulates the subsequent biological cascade of proliferation, differentiation, extracellular matrix production (ECM) and matrix mineralization [113][114][115][116]. Unsuccessful attachment of osteogenic cells can even cause dedifferentiation and loss of their phenotype [117][118].…”