Osteoarthritis as a Whole Joint Disease

Poole, A R

doi:10.1007/s11420-011-9248-6

Cited by 120 publications

(105 citation statements)

References 19 publications

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“…Osteoarthritis (OA) is a major, chronic joint disorder that involves deep changes in the structure and functions of skeletal tissues and cells, causing the gradual and irreversible breakdown of the articular cartilage (the end point of OA), the remodeling of the subchondral bone, and the formation of osteophytes 1,2 by an impaired homeostasis. 3,4 Current pharmacological and surgical strategies cannot fully reverse the OA phenotype and the original cartilage integrity is never being recovered.…”

Section: Introductionmentioning

confidence: 99%

rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

Rey‐Rico

Venkatesan

Schmitt

et al. 2017

IJN

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Recombinant adeno-associated virus (rAAV) vectors are clinically adapted vectors to durably treat human osteoarthritis (OA). Controlled delivery of rAAV vectors via polymeric micelles was reported to enhance the temporal and spatial presentation of the vectors into their targets. Here, we tested the feasibility of delivering rAAV vectors via poly (ethylene oxide) (PEO) and poly (propylene oxide) (PPO) (poloxamer and poloxamine) polymeric micelles as a means to overexpress the therapeutic factor transforming growth factor-beta (TGF-β) in human OA chondrocytes and in experimental human osteochondral defects. Application of rAAV-human transforming growth factor-beta using such micelles increased the levels of TGF-β transgene expression compared with free vector treatment. Overexpression of TGF-β with these systems resulted in higher proteoglycan deposition and increased cell numbers in OA chondrocytes. In osteochondral defect cultures, a higher deposition of type-II collagen and reduced hypertrophic events were noted. Delivery of therapeutic rAAV vectors via PEO-PPO-PEO micelles may provide potential tools to remodel human OA cartilage.

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Section: Introductionmentioning

confidence: 99%

rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

Rey‐Rico

Venkatesan

Schmitt

et al. 2017

IJN

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“…It not only affects the articular cartilage and subchondral bone but also involves other structures of the joint, including menisci, synovial membrane, joint capsule, ligaments, muscles and infrapatellar fat pad (IPFP) 2 3…”

Section: Introductionmentioning

confidence: 99%

Signal intensity alteration in the infrapatellar fat pad at baseline for the prediction of knee symptoms and structure in older adults: a cohort study

et al. 2015

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“…However, other studies reported no difference in IFP size between OA patients and controls (4) or an increased IFP size in patients with patellofemoral OA, with a positive relationship between IFP volume and pain (5). The overall role of the IFP in joint health and OA is thus unclear (6), motivating specific studies on metabolic interactions with other tissues.…”

Section: Introductionmentioning

confidence: 99%

“…Degenerative disorders of articular joints are now widely viewed as “whole joint” diseases, and both the maintenance and degeneration of joints involve direct and indirect interactions among multiple joint tissues (6, 7). In vitro models allow exploration of potential interactions between tissues, and have been useful for studying effects of joint capsular cells and tissues on articular cartilage metabolism.…”

Section: Introductionmentioning

confidence: 99%

Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues

Nishimuta

Bendernagel

Levenston

2016

Connective Tissue Research

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Purpose: Although osteoarthritis is widely viewed as a disease of the whole joint, relatively few studies have focused on interactions among joint tissues in joint homeostasis and degeneration. In particular, few studies have examined effects of the infrapatellar fat pad (IFP) on cartilaginous tissues. The aim of this study was to test the hypothesis that co-culture with healthy IFP would induce degradation of cartilage and meniscus tissues. Materials and Methods: Bovine articular cartilage, meniscus, and IFP were cultured isolated or as cartilage-fat or meniscus-fat co-cultures for up to 14 days. Conditioned media were assayed for sulfated glycosaminoglycan (sGAG) content, nitrite content and matrix metalloproteinase (MMP) activity, and explants were assayed for sGAG and DNA contents. Results: Co-cultures exhibited increased cumulative sGAG release and sGAG release rates for both cartilage and meniscus, and cartilage (but not meniscus) exhibited a substantial synergestic effect of co-culture (sGAG release in co-culture was significantly greater than the summed release from isolated cartilage and fat). Fat co-culture did not significantly alter sGAG content of either cartilage or meniscus explants, indicating that IFP co-culture stimulated net sGAG production by cartilage. Nitrite release was increased relative to isolated tissue controls in co-cultured meniscus but not cartilage, with no synergistic effect of co-culture. Interestingly, MMP-2 production was decreased by co-culture for both cartilage and meniscus. Conclusions: This study demonstrates that healthy IFP may modulate joint homeostasis by stimulating sGAG production in cartilage. Counter to our hypothesis, healthy IFP did not promote degradation of either cartilage or meniscus tissue.

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Osteoarthritis as a Whole Joint Disease

Cited by 120 publications

References 19 publications

rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

Signal intensity alteration in the infrapatellar fat pad at baseline for the prediction of knee symptoms and structure in older adults: a cohort study

Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues

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Osteoarthritis as a Whole Joint Disease

Cited by 120 publications

References 19 publications

rAAV-mediated overexpression of TGF-&beta;&nbsp;via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

rAAV-mediated overexpression of TGF-&beta;&nbsp;via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

Signal intensity alteration in the infrapatellar fat pad at baseline for the prediction of knee symptoms and structure in older adults: a cohort study

Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues

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rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model

rAAV-mediated overexpression of TGF-β via vector delivery in polymeric micelles stimulates the biological and reparative activities of human articular chondrocytes in vitro and in a human osteochondral defect model