Osteoarthritis‐alleviating effects in papain‐induced model rats of chicken cartilage hydrolysate and its peptide fractions

Abstract

This study aims to discover natural bioactive substances as alternative therapies to current medical and surgical treatments for osteoarthritis (OA). Chicken cartilage hydrolysate (CCH) was produced using trypsin and separated via ultrafiltration into CCH-I (molecular weight (MW) > 10 kDa) and CCH-II (MW <10 kDa). Results showed that CCH and its fractions (CCH-I and CCH-II) could relieve OA with different extents, and the outcome using these treatments depended on their especial composition. CCH-II (a fraction rich in low MW peptides with no chondroitin sulphate (CS) and low in sugar) exhibited desired OA-alleviating effect in papain-induced rat and highest in vitro antioxidant activities. CCH-I (a fraction rich in CS and in high MW peptides) might have an adverse effect on renal function. Therefore, it is industrially feasible to produce anti-inflammatory and antioxidative CCH, especially CCH-II, with the therapeutic potential for alleviating or treating OA. Further separation of CS from CCH-I may lead to a commercial pure CS product for treating OA.

Food-derived bioactive peptides potentiating therapeutic intervention in rheumatoid arthritis

“… Rabbits Lee et al [ 137 ] Pilose antler ( Cervus elaphus Linnaeus) peptide Deer antlers In vitro , 1–128 μM, 24 h Over-expression of inflammatory factors was inhibited Nucleus pulposus of the vertebra disc Dong et al [ 138 ] Chicken cartilage hydrolysate (Mw < 10 kDa) Chicken Oral, 100 mg/kg/day, 30 days IL-1β, IL-10, TNF-α and MMP-13 levels decreased. Rats Yang et al [ 139 ] Antarctic Krill peptides Antarctic Krill Oral, 195–600 mg/kg/day, 8 weeks Cartilage thickness and area increased. Rats Wang et al [ 140 ] Lunasin Soybean In vitro , 50 and 100 μM, 48 h MMP-3 and MMP-13 decreased.…”

Bioactive peptides: an alternative therapeutic approach for cancer management

“…The pathogenesis of osteoarthritis involves the activation of the inflammatory cascade through the stimulation of various cytokines such as TNF-α, IL-10, IL-8, and IL-1β. The study found that the hydrolysis of trypsin in chicken cartilage resulted in a reduction of these cytokine in papain-induced model rats, leading to an alleviation of osteoarthritis symptoms ( 152 ). The silkworm pupa ( Bombyx mori ) protein is rich in valuable peptides.…”

Antioxidant and α-Amylase Inhibitory Properties Generated from Chicken Head Proteins by Dual-Enzyme Hydrolysis

“…In this study, it was seen that CHP1 using 0.25% bromelain and 0.75% papain had the strongest antioxidant activity when compared with other samples. Yang et al (2019) reported that chicken cartilage hydrolysate, fractions >10 kDa, and <10 kDa using trypsin for 3 hours had IC 50 values of 9.80, 18.91, and 7.35 mg/ml, while Alahyaribeik et al (2021) reported that the <3 and <10 kDa fractions from chicken feather hydrolysate using fermented by Bacillus licheniformis had IC 50 values of 5.03 and 6.64 mg/ml, respectively. This showed that chicken head protein hydrolysate had antioxidant activity that was equivalent or even better than chicken cartilage and feather hydrolysate.…”

“…Antioxidant peptides can come from vegetable proteins such as legumes, or from animal proteins from muscle tissue, milk, egg, and by-products. Bioactive peptides derived from poultry by-products, such as chicken skin (Onuh et al, 2014), chicken cartilage (Yang et al, 2019), chicken blood (Hamzeh et al, 2019) and chicken feathers (Alahyaribeik et al, 2021) showed the ability and potential as antioxidant. Bioactive peptides have good electron donor ability and hydrophobic character, so they can act as antioxidants by donating electrons to free radicals and converting them into stable compounds (Najafian and Babji, 2014).…”