Osteoanagenesis after transplantation of bone marrow-derived mesenchymal stem cells using polyvinylidene chloride film as a scaffold

Abstract

The aim of this study was to develop a new cell transplantation technique for osteoanagenesis at bone defect sites. Polyvinylidene chloride (PVDC) film was evaluated because of its good biocompatibility and flexibility. We used this film as both a cell scaffold and a barrier membrane. Initially, the cell compatibility of the PVDC film for fibroblast-like cells and osteoblast-like cells was confirmed. Subsequently, bone marrow cells were obtained from rats and cultured on PVDC films in two kinds of medium. The PVDC films with bone marrow-derived mesenchymal stem cells (MSCs) were then applied to critical-sized bone defects in the calvarial bone of rats. After the transplantation, the surgical sites were dissected out and evaluated by soft X-ray radiography, micro-CT analysis and histological examinations. The bone marrow-derived MSC-transplanted rats showed greater bone regeneration than the control rats. Therefore, PVDC film is considered to be useful as a scaffold for bone regeneration.

Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids

“…We investigated the effectiveness of implanting human MSC-spheroids prepared by a simple rotation culture without using a 3D matrix in the repair of critical-sized bone defects (8 mm) in the rat calvaria. Previous studies on calvarial bone regeneration have involved the use of genetically engineered MSCs [ 30 – 33 ], and various synthetic biodegradable 3D scaffolds for MSC implantation, including polyvinylidene chloride (PVDC) films [ 34 ], natural bone mineral (Bio-Oss) with β-TCP [ 35 ], and poly (lactide-co-glycolide) scaffolds [ 36 ] have been used. CP ceramics, including β-TCP and hydroxyapatite, are effective as scaffolds for BM-derived MSCs for bone regeneration and vascularization [ 37 , 38 ], with hydroxyapatite affording greater bone volume than β-TCP.…”

Controlled formation of polylysinized inner pores in injectable microspheres of low molecular weight poly(lactide-co-glycolide) designed for efficient loading of therapeutic cells

“…However, the vulnerable and sensitive nature of the cells often leads to low cell survival, delivery efficiency, and engraftment rate to host tissues, limiting their therapeutic effects [1]. To address the problems, diverse cell delivery systems have been investigated, such as micro-particulate carriers [2], hydrogels [3] and films [4].…”

Study of Mesenchymal Stem Cells Cultured on a Poly(Lactic-co-Glycolic Acid) Scaffold Containing Simvastatin for Bone Healing

“…The aging population in the world makes bone fractures a substantial public health issue, thus increasing the need for strategies for bone regeneration (1). Autologous grafts (i.e., tissue or organs of the individual) remain the gold standard for stimulating bone regeneration (2), but these procedures are limited by lack of donor area, by morbidity and by pain. Therefore, development of artificial bone is indispensable.…”