Osteo Growth Induction titanium surface treatment reduces ROS production of mesenchymal stem cells increasing their osteogenic commitment

Ghensi, Paolo; Bressan, Eriberto; Gardin, Chiara; Ferroni, Letizia; Ruffato, Lucio; Caberlotto, Mauro; Soldini, Claudio; Zavan, Barbara

doi:10.1016/j.msec.2016.12.032

Cited by 16 publications

(14 citation statements)

References 21 publications

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“…Adhesion and bio lm formation at the implant surface due to oral microbiota or pathogenic microorganisms introduced during or after surgery is one of the main causes of dental implants failure [56,57]. The formation and preservation of an effective mucosal seal around the transmucosal components [58][59][60] and a quick osseointegration process [60,61] are essential for limiting the microbial migration to the coronal portion of the implant. Therefore, effective solutions for oral implantology should inhibit microbial cell attachment and bio lm formation at the implant surface preserving biocompatibility and bioactive properties required for the integration with the peri-implant tissues.…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Tambone

Bonomi

Ghensi

et al. 2020

Preprint

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Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new of anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods: R89BS was physically adsorbed on titanium discs (TDs) and the ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile cells was also performed by scanning electron microscopy. Results: R89BS-coated discs showed no cytotoxic effects on normal lung fibroblasts (MRC5). TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 98%, 49% and 10% and of S. epidermidis by 53%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 75% for S. epidermidis at 24 h. Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.

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Section: Discussionmentioning

confidence: 99%

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Tambone

Bonomi

Ghensi

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Adhesion and bio lm formation at the implant surface due to oral microbiota or pathogenic microorganisms introduced during or after surgery is one of the main causes of dental implants failure [45,46]. The formation and preservation of an effective mucosal seal around the transmucosal components [47][48][49] and a quick osseointegration process [49,50] are essential for limiting the microbial migration to the coronal portion of the implant. Therefore, effective solutions for oral implantology should inhibit microbial cell attachment and bio lm formation at the surface of the implant and of transmucosal components preserving biocompatibility and bioactive properties required for the integration with the peri-implant tissues.…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in-vitro study

Tambone

Bonomi

Ghensi

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods: R89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5). using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy. Results: R89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 98%, 49% and 10% and of S. epidermidis by 53%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 75% for S. epidermidis at 24 h. Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.

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“…This could reduce biocompatibility. However, OGI Titanium (Ti) surface microarchitecture has been shown to increase osteoblast differentiation [ 28 , 29 , 30 , 31 , 32 ] by our previous publication.…”

Section: Discussionmentioning

confidence: 99%

“…In the end there is an intimate connection between endothelial cells and bone cells, and information of blood vessels in bone enhances osteogenesis that takes place in the neighborhood of newly formed vessels [ 27 ]. Several titanium surfaces are available, and in this scenario in the present work we have better characterized the biological properties of a novel dental surfaces OGI that we have previously characterized [ 28 ] by mechanical point of view. OGI implants are characterized by a large-grit sandblasted and acid etched surface (Ra.…”

Section: Introductionmentioning

confidence: 99%

The Biological Properties of OGI Surfaces Positively Act on Osteogenic and Angiogenic Commitment of Mesenchymal Stem Cells

et al. 2017

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Osteogenesis process displays a fundamental role during dental implant osteointegration. In the present work, we studied the influence of Osteon Growth Induction (OGI) surface properties on the angiogenic and osteogenic behaviors of Mesenchymal Stem cells (MSC). MSC derived from dental pulp and HUVEC (Human Umbilical Vein Endothelial Cells) were grown in on OGI titanium surfaces, and cell proliferation and DNA synthesis were evaluated by MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] test and DNA quantification. Gene expression has been performed in order to evaluate the presence of mRNA related to endothelial and osteogenesis markers. Moreover, morphological and biochemical analyses of osteogenesis commitments has been performed. On OGI surfaces, MSC and HUVEC are able to proliferate. Gene expression profiler confirms that MSC on OGI surfaces are able to express endothelial and osteogenic markers, and that these expression are higher compared the expression on control surfaces. In conclusion On OGI surfaces proliferation, expression and morphological analyses of angiogenesis-associated markers in MSC are promoted. This process induces an increasing on their osteogenesis commitment.

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Osteo Growth Induction titanium surface treatment reduces ROS production of mesenchymal stem cells increasing their osteogenic commitment

Cited by 16 publications

References 21 publications

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in-vitro study

The Biological Properties of OGI Surfaces Positively Act on Osteogenic and Angiogenic Commitment of Mesenchymal Stem Cells

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