OSTE+ for <i>in situ</i> SAXS analysis with droplet microfluidic devices

Lange, Tobias; Charton, Sophie; Bizien, Thomas; Testard, Fabienne; Malloggi, Florent

doi:10.1039/d0lc00454e

Cited by 15 publications

(23 citation statements)

References 44 publications

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“…Chitosan electrodeposition was successfully detected, opening NMR to other electrochemical applications. Other papers reported the coupling of microfluidics to small-angle X-ray scattering or time-resolved cryo-EM. , …”

Section: Detectionmentioning

confidence: 99%

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

et al. 2020

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Section: Detectionmentioning

confidence: 99%

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

et al. 2020

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“…As the main characteristics, these devices show reduced size, low weight, and high throughput. These characteristics have attracted tremendous and widespread attention by the synchrotron community 2 , 3 interested in compatible instrumentation for in situ and in vivo experiments. To date, reported applications of these devices on synchrotrons, involving studies on protein crystallography 4 , 5 , advanced materials 6 , electrochemistry 7 , 8 , catalysis 9 – 11 , in vivo experiments (eukaryotic and bacteria cells) 6 , 12 , 13 , established a paramount experimental contribution for drawing a picture of these complexes systems, providing precious information about electronic, structural, and chemical properties in real-time, in situ and/or operando.…”

Section: Introductionmentioning

confidence: 99%

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

Neckel

Castro

Callefo

et al. 2021

Sci Rep

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Shedding synchrotron light on microfluidic systems, exploring several contrasts in situ/operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

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“…As the main characteristics, these devices show reduced size, low weight, and high throughput. These characteristics have attracted tremendous and widespread attention by the synchrotron community 2,3 interested in compatible instrumentation for in situ and in vivo experiments. To date, reported applications of these devices on synchrotrons, involving studies on protein crystallography 4,5 , advanced materials 6 , electrochemistry 7,8 , catalysis [9][10][11] , in vivo experiments (eukaryotic and bacteria cells) 6,12,13 , established a paramount experimental contribution for drawing a picture of these complexes systems, providing precious information about electronic, structural, and chemical properties in real-time, in situ and/or operando.…”

Section: Introductionmentioning

confidence: 99%

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

Neckel

Castro

Callefo

et al. 2021

Preprint

View full text Add to dashboard Cite

Shedding synchrotron light on microfluidic systems, exploring several contrasts in situ operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

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OSTE+ for in situ SAXS analysis with droplet microfluidic devices

Abstract:
In recent years, microfluidic-based sample preparation techniques have emerged as a powerful tool for measurements at large scale X-ray facilities. Most often the microfluidic device was a form of hybrid...

Cited by 15 publications

References 44 publications

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

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About

OSTE+ for in situ SAXS analysis with droplet microfluidic devices

Abstract: In recent years, microfluidic-based sample preparation techniques have emerged as a powerful tool for measurements at large scale X-ray facilities. Most often the microfluidic device was a form of hybrid...

Cited by 15 publications

References 44 publications

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

Contact Info

Product

Resources

About

Abstract:
In recent years, microfluidic-based sample preparation techniques have emerged as a powerful tool for measurements at large scale X-ray facilities. Most often the microfluidic device was a form of hybrid...