OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice

Shi, Yi; Chang, Yanli; Wu, Haixia; Shalmani, Abdullah; Liu, Wenting; Li, Wenqiang; Xu, Jianwei; Chen, Kun-Ming

doi:10.1007/s00299-020-02603-2

Cited by 47 publications

(33 citation statements)

References 67 publications

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“…The integration of NADPH oxidases and ROS as important messengers in ABA signaling governs plant molecular responses to drought and requires the aforementioned dedicated balance between ROS production and scavenging (Kwak et al, 2003;Postiglione and Muday, 2020). The importance of actively produced ROS in stomatal closure has been also evidenced in rice (Oryza sativa) knockout plants lacking the drought-inducible NADPH oxidases OsRbohA or OsRbohB that displayed an impaired drought tolerance, whereas OsRbohA overexpression increased the survival rates under severe drought stress (Shi et al, 2020;Wang et al, 2016a). NADPH oxidases release O 2 in the apoplast that is subsequently converted spontaneously or by an apoplastic SOD to H 2 O 2 .…”

Section: Improving Drought Stress Resilience Through Engineering Of the Antioxidant Machinery And Redox-related Mechanismsmentioning

confidence: 97%

Improving oxidative stress resilience in plants

Kerchev

Breusegem

2021

The Plant Journal

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Originally conceived as harmful metabolic byproducts, reactive oxygen species (ROS) are now recognized as an integral part of numerous cellular programs. Thanks to their diverse physicochemical properties, compartmentalized production, and tight control exerted by the antioxidant machinery they activate signaling pathways that govern plant growth, development, and defense. Excessive ROS levels are often driven by adverse changes in environmental conditions, ultimately causing oxidative stress. The associated negative impact on cellular constituents have been a major focus of decade-long research efforts to improve the oxidative stress resilience by boosting the antioxidant machinery in model and crop species. We highlight the role of enzymatic and non-enzymatic antioxidants as integral factors of multiple signaling cascades beyond their mere function to prevent oxidative damage under adverse abiotic stress conditions.

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Section: Improving Drought Stress Resilience Through Engineering Of the Antioxidant Machinery And Redox-related Mechanismsmentioning

confidence: 97%

Improving oxidative stress resilience in plants

Kerchev

Breusegem

2021

The Plant Journal

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“…Since the plasma membrane NADPH oxidase, also known as respiratory burst oxidase homologs (Rbohs), play vital roles in plant stress tolerance (Hu et al 2020a , b ; Shi et al 2020 ) and OsRbohA, a key NADPH oxidase for ROS production, functions in the developmental regulation and drought-stress tolerance of rice (Wang et al 2016 ), we then tested whether the functions of OsCBM1 in drought tolerance and ROS production are related to OsRbohA. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Rice Carbohydrate-Binding Malectin-Like Protein, OsCBM1, Contributes to Drought-Stress Tolerance by Participating in NADPH Oxidase-Mediated ROS Production

Jing

Zhou

et al. 2021

Rice

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Carbohydrate-binding malectin/malectin-like domain-containing proteins (CBMs) are a recently identified protein subfamily of lectins that participates various functional bioprocesses in the animal, bacterial, and plant kingdoms. However, little is known the roles of CBMs in rice development and stress response. In this study, OsCBM1, which encodes a protein containing only one malectin-like domain, was cloned and characterized. OsCBM1 is localized in both the endoplasmic reticulum and plasma membrane. Its transcripts are dominantly expressed in leaves and could be significantly stimulated by a number of phytohormone applications and abiotic stress treatments. Overexpression of OsCBM1 increased drought tolerance and reactive oxygen species production in rice, whereas the knockdown of the gene decreased them. OsCBM1 physically interacts with OsRbohA, a NADPH oxidase, and the expression of OsCBM1 in osrbohA, an OsRbohA-knockout mutant, is significantly downregulated under both normal growth and drought stress conditions. Meanwhile, OsCBM1 can also physically interacts with OsRacGEF1, a specific guanine nucleotide exchange factor for the Rop/Rac GTPase OsRac1, and transient coexpression of OsCBM1 with OaRacGEF1 significantly enhanced ROS production. Further transcriptome analysis showed that multiple signaling regulatory mechanisms are involved in the OsCBM1-mediated processes. All these results suggest that OsCBM1 participates in NADPH oxidase-mediated ROS production by interacting with OsRbohA and OsRacGEF1, contributing to drought stress tolerance of rice. Multiple signaling pathways are likely involved in the OsCBM1-mediated stress tolerance in rice.

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“…These results indicated that ROC22 may have more lasting antioxidant capacity. ROS also play a role in signal transduction mechanism in the response to stress [ 37 ]. H 2 O 2 as a kind of ROS produced by RBOHF could regulate guard cell signaling and stomatal closure [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Gene Co-Expression Analysis Reveals Transcriptome Divergence between Wild and Cultivated Sugarcane under Drought Stress

Lin

Zhong

et al. 2022

IJMS

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Drought is the main abiotic stress that constrains sugarcane growth and production. To understand the molecular mechanisms that govern drought stress, we performed a comprehensive comparative analysis of physiological changes and transcriptome dynamics related to drought stress of highly drought-resistant (ROC22, cultivated genotype) and weakly drought-resistant (Badila, wild genotype) sugarcane, in a time-course experiment (0 h, 4 h, 8 h, 16 h and 32 h). Physiological examination reviewed that ROC22, which shows superior drought tolerance relative to Badila, has high performance photosynthesis and better anti-oxidation defenses under drought conditions. The time series dataset enabled the identification of important hubs and connections of gene expression networks. We identified 36,956 differentially expressed genes (DEGs) in response to drought stress. Of these, 15,871 DEGs were shared by the two genotypes, and 16,662 and 4423 DEGs were unique to ROC22 and Badila, respectively. Abscisic acid (ABA)-activated signaling pathway, response to water deprivation, response to salt stress and photosynthesis-related processes showed significant enrichment in the two genotypes under drought stress. At 4 h of drought stress, ROC22 had earlier stress signal transduction and specific up-regulation of the processes response to ABA, L-proline biosynthesis and MAPK signaling pathway–plant than Badila. WGCNA analysis used to compile a gene regulatory network for ROC22 and Badila leaves exposed to drought stress revealed important candidate genes, including several classical transcription factors: NAC87, JAMYB, bHLH84, NAC21/22, HOX24 and MYB102, which are related to some antioxidants and trehalose, and other genes. These results provide new insights and resources for future research and cultivation of drought-tolerant sugarcane varieties.

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OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice

Cited by 47 publications

References 67 publications

Improving oxidative stress resilience in plants

Improving oxidative stress resilience in plants

Rice Carbohydrate-Binding Malectin-Like Protein, OsCBM1, Contributes to Drought-Stress Tolerance by Participating in NADPH Oxidase-Mediated ROS Production

Gene Co-Expression Analysis Reveals Transcriptome Divergence between Wild and Cultivated Sugarcane under Drought Stress

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