Osobennosti khronicheskikh ran u bol'nykh sakharnym diabetom i puti ikh korrektsii

Tokmakova, A Yu; Strakhova, G Yu; Arbuzova, M I

doi:10.14341/2306-3513-2007-1-38-42

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Раневые дефекты у больных с СДС считаются «замершими», в одних случаях на фазе воспаления, в других -пролиферации. Существует мнение, что в подобных случаях происходит чрезмерная экспрессия фибронектина и тромбоспондина, что приводит к клеточной дисфункции и дизрегуляции [28].…”

Section: сахарный диабет Diabetes Mellitusunclassified

Neurohumoral mechanisms of keratinocytes regulation in diabetes mellitus

Artemova¹,

Горбачева²,

Galstyan³

et al. 2016

Diabetes mellitus

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The extent of damage to the nervous, vascular and microcirculatory systems in diabetic patients determine the regulation of physiological events that lead to the formation of chronic wounds, reduction of patient quality of life and increase of the financial value of medical care. Successful physiological repair is impossible without the successive phases of inflammation, proliferation and wound healing. Keratinocytes are the major cellular barrier components of the epidermis. These cells play an important role in physiological repair, as suggested by recent research, with many cells able to secrete steroid hormones de novo. Damage to the integrity of the skin leads to keratinocyte activation, triggering a cascade of reactions that contribute to changes in epidermal cell phenotype and lead to their proliferation and migration, analogous to changes in cellular adhesion and configuration of the cytoskeleton. An open question remains as to how the keratinocyte cell cycle, which is altered under conditions of hyperglycemia, and neurotransmitter metabolism during different stages of physiological repair are regulated. Understanding these processes will provide a scientific basis for the development of new targets for pharmacotherapies.

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Section: сахарный диабет Diabetes Mellitusunclassified

Neurohumoral mechanisms of keratinocytes regulation in diabetes mellitus

Artemova¹,

Горбачева²,

Galstyan³

et al. 2016

Diabetes mellitus

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“…На сегодняшний день более 460 млн людей во всем мире страдают сахарным диабетом; по прогнозам Международной федерации диабета к 2040-му году число больных возрастет до 642 млн [1].…”

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Wound healing mechanisms in rats with streptozotocin-induced diabetes mellitus

Иванов¹,

Гаврилова²,

Морозова³

et al. 2018

Probl Endokrinol (Mosk)

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Background. Wound healing disorders and formation of diabetic foot, a severe disabling complication of diabetes mellitus, are accompanied by nervous system impairment and/or ischemia. Objective — the study was aimed at assessing the effect of peripheral innervation disorders on the regulation of tissue repair in the streptozotocin-induced rat model of diabetes mellitus. Material and methods. The study was carried out in male white outbred rats (n=70). The animals were wounded 42 days after induction of diabetes by injecting streptozotocin (diabetes group; this group received insulin Levemir at a dose of 2 units/kg in saline subcutaneously to reduce mortality), or after injection of citrate buffer (CB group). Skin samples were taken on day 8, 16, and 24 after wound modeling. Pain sensitivity was assessed in all animals. The resulting skin fragments were fixed, dehydrated, and embedded in paraffin according to standard procedures. Sections were stained with hematoxylin and eosin, antibodies specific for Ki-67, α1, β1, and β2-adrenoreceptors were used for immunohistochemical staining. Intact animals were used as an additional control group. Results. Tail withdrawal time measured on day 56 was higher in DM group rats as compared to the control group (p=0.017). CB group demonstrated a tendency towards more rapid wounds healing than diabetic animals, although the difference was not statistically significant due to wide scatter of data in the DM group (p=0.64). The intensity of staining for Ki67 was lower in the DM group (p=0.045). Reduced density of β2-adrenoreceptors was observed at the areas remote from the wound in CB group rats. Conclusion The results show no correlation between altered innervation and impaired tissue repair in rats with streptozotocin-induced diabetes.

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Influence of peripheral nerve system on proliferation and migration of keratinocytes on site of the wound edges

Artemova,

Dzhemilova,

Gorbacheva

et al. 2024

Diabetes mellitus

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AIM: to assess proliferation and migration of keratinocytes at the nonhealing edges of neuropathic wounds.MATERIALS AND METHODS: 25 patients with neuropathic ulcers and 5 patients without diabetes with decubitus were enrolled. Diabetic foot (DF) patients were underwent to standard treatment including debridement, atraumatic dressing, offloading, antibacterial therapy if it needs. Severity of peripheral neuropathy was assessed according to the NDS scale. Histological (hematoxylin and eosin) and immunohistochemical (Ki-67 , α7nAChR markers) examination of wound edge were done during treatment (0, 10, 24 days).RESULTS: All patients have severe neuropathy according to NDSm (>8). The average size of DF ulcers before and on 10th day of treatment was of 4 cm2 and 2,5 cm2, respectively (p<0,004). Neuropathic ulcers were characterized by hyperproliferative epidermis. Mitotically active keratinocytes reside throughout the suprabasal layers. Ki-67 expressed all layers of the epidermis, but a greater staining density was detected in the basal layer. The density of a7nAChR-positive cells increased from 0 to 24 days (p=0,031).THE CONCLUSION: The data shows that neuropathy is one of the possible mechanisms of keratinocyte cell cycle disruption: proliferative activity and ability to migrate. Identification of new signaling pathways regulating the physiological repair of tissues and the study of their disorders in diabetes mellitus opens the prospect of developing an optimal therapeutic strategy.

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Osobennosti khronicheskikh ran u bol'nykh sakharnym diabetom i puti ikh korrektsii

Cited by 3 publications

References 13 publications

Neurohumoral mechanisms of keratinocytes regulation in diabetes mellitus

Neurohumoral mechanisms of keratinocytes regulation in diabetes mellitus

Wound healing mechanisms in rats with streptozotocin-induced diabetes mellitus

Influence of peripheral nerve system on proliferation and migration of keratinocytes on site of the wound edges

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